Dietary Fatty Acids and Membrane Protein Function

Murphy, Mary

doi:10.1016/0955-2863(90)90052-m

Cited by 254 publications

(107 citation statements)

References 87 publications

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“…No study has considered in detail the release of long-chain saturated, monounsaturated and polyunsaturated fatty acids (PUFA). This omission represents a major gap in our knowledge, because adipose tissue is the reservoir of fatty acids used as energy substrates, notably during energy depletion, and also as components of cell membranes (Murphy, 1990;Clandinin et al 1991) from which some of them may be used as precursors of eicosanoids (Bruckner, 1992;Lands, 1992). Thus, whether fatty acids are randomly or selectively released during lipolysis, and how the molecular structure of fatty acids affects their mobilization rates from fat cells, has been a subject of debate.…”

Section: Selectivity Of Individual Fatty Acid Storage and Mobilizationmentioning

confidence: 99%

Selectivity of fatty acids on lipid metabolism and gene expression

Raclot¹,

Oudart²

1999

Proc. Nutr. Soc.

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Triacylglycerols represent the main form of storage for a wide spectrum of fatty acids. Their utilization first involves mobilization from adipose tissue through lipolysis. The release of individual fatty acids from adipose tissue is selective in vitro and in vivo in animal studies and also in human subjects. Generally, fatty acids are more readily mobilized from fat cells when they are short-chain and unsaturated. This selectivity could affect the storage of individual fatty acids in adipose tissue, and their subsequent supply to tissues. The nature of the dietary fats could affect lipid homeostasis and body fat deposition. Dietary fish oil influences adipose tissue development in a site-specific manner as a function of diet and feeding period. A diet high in n-3 polyunsaturated fatty acids (PUFA) results in a preferential partitioning of ingested energy towards oxidation at the expense of storage. Fatty acids are important mediators of gene expression in the liver. Indeed, genes encoding both glycolytic and lipogenic enzymes and key metabolic enzymes involved in fatty acid oxidation are regulated by dietary PUFA. White adipose tissue could also be a target for PUFA control of gene expression. The treatment of pre-adipose cells by fatty acids induces the expression of numerous genes that encode proteins involved in fatty acid metabolism. The mechanisms of PUFA-mediated repression of gene expression in adipocytes seem to be different, at least partly, from those described in liver. Tissue-specific and site-specific factors are possibly involved in the specific effect of PUFA on gene expression, although other mechanisms cannot be excluded.

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Section: Selectivity Of Individual Fatty Acid Storage and Mobilizationmentioning

confidence: 99%

Selectivity of fatty acids on lipid metabolism and gene expression

Raclot¹,

Oudart²

1999

Proc. Nutr. Soc.

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“…Phospholipids are the main constituents of biological membranes and their fatty acid * Corresponding author. Email: marina@ugr.es composition can influence the function of membrane proteins, such as receptors, enzymes, transporters or ion channels (Murphy 1990). The fatty acyl chains of the phospholipids are chemical determinants of the fluidity of the lipid bilayer and, in that way, produce changes in the functioning of those proteins whose actions depend on mobility within the plane of the membranes.…”

Section: Introductionmentioning

confidence: 99%

Influence of environmental medium on membrane fatty acid composition of Reuber H35 hepatoma cells

García-Pelayo

Garcia-Peregrin

Martínez-Cayuela

2013

Frontiers in Life Science

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Cell membrane proteins are sensitive to their fatty acid environment; however, the effects of those fatty acids are not completely understood. In this work, we have optimized a model for the study of membrane protein regulation, which allows the investigation of lipid metabolism in cultured tumor cells. The membrane fatty acid composition of Reuber H35 hepatoma cells was modified by enriching phospholipids in individual fatty acids of different length and degree of saturation. Lauric, myristic, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were supplemented to the culture medium of cells growing in presence or absence of lipoproteins. The four fatty acids were incorporated into membrane phospholipids in a dosedependent manner. Lauric and myristic acids were actively metabolized, increasing percentages of longer and unsaturated chain fatty acids, but they did not modify the unsaturated index. As expected, EPA and DHA significantly decreased the unsaturated index. Incorporation of EPA and DHA was accompanied of a significant decrease of total monounsaturated fatty acids and an increase of total saturated fatty acids. Our results demonstrate that Reuber H35 hepatoma cells constitute a suitable model to manipulate the lipid composition of the cell membranes and perform functionality studies on the membrane proteins in response to their lipid environment.

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“…The composition of the phospholipid bilayer can vary greatly, and this affects the functional properties of membrane proteins by altering their local molecular environment [1][2][3][4]. Changing the composition of membrane phospholipids modulates the activity of many oxidative enzymes, ATPases, hormone receptors and ion channels [5][6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Setting the pace of life: membrane composition of flight muscle varies with metabolic rate of hovering orchid bees

et al. 2015

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Patterns of metabolic rate variation have been documented extensively in animals, but their functional basis remains elusive. The membrane pacemaker hypothesis proposes that the relative abundance of polyunsaturated fatty acids in membrane phospholipids sets the metabolic rate of organisms. Using species of tropical orchid bees spanning a 16-fold range in body size, we show that the flight muscles of smaller bees have more linoleate (%18 : 3) and stearate (%18 : 0), but less oleate (%18 : 1). More importantly, flight metabolic rate (FlightMR) varies with the relative abundance of 18 : 3 according to the predictions of the membrane pacemaker hypothesis. Although this relationship was found across large differences in metabolic rate, a direct association could not be detected when taking phylogeny and body mass into account. Higher FlightMR, however, was related to lower %16 : 0, independent of phylogeny and body mass. Therefore, this study shows that flight muscle membrane composition plays a significant role in explaining diversity in FlightMR, but that body mass and phylogeny are other factors contributing to their variation. Multiple factors are at play to modulate metabolic capacity, and changing membrane composition can have gradual and stepwise effects to achieve a new range of metabolic rates. Orchid bees illustrate the correlated evolution between membrane composition and metabolic rate, supporting the functional link proposed in the membrane pacemaker hypothesis.

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Dietary Fatty Acids and Membrane Protein Function

Cited by 254 publications

References 87 publications

Selectivity of fatty acids on lipid metabolism and gene expression

Selectivity of fatty acids on lipid metabolism and gene expression

Influence of environmental medium on membrane fatty acid composition of Reuber H35 hepatoma cells

Setting the pace of life: membrane composition of flight muscle varies with metabolic rate of hovering orchid bees

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