Dietary flavone chrysin (5,7-dihydroxyflavone ChR) functionalized highly-stable metal nanoformulations for improved anticancer applications

Abstract

Nanomaterials of noble metals with unique size, shape and composition receives much attention owing to their versatile functionality in personalized cancer nanomedicine. Chrysin (ChR), a natural anticancer bioflavonoid emerged as potential drug therapy for almost all types of cancer, however it has poor solubility and bioavailability. Herein, we report a new approach to formulate biofunctionlized metallic silver (ChR-AgNPs) and gold (ChR-AuNPs) nanoparticles using ChR as direct bioreductant and capping agent. Size and dispersity of nanoparticles (NPs) were controlled through fixing different reaction conditions such as temperature, pH, concentration of metal ion, stochiometric proportion of reaction mixture and incubation time based on their optical properties and SPR effect in UV-visible spectroscopy. Role of hydroxyl and carbonyl groups in functionalizing metal ions with ChR was confirmed with Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) analysis. It also substantiate that oxygen group from ChR donates electron to metal ion and results in complexation, ionic Ag + and Au 3+ were reduced to Ag 0 and Au 0 nano-forms. Physiochemical state of obtained NPs were characterized through different exclusive instrumentation, which shows the presence of highly-stable, spherical, crystalline ChR-AgNPs with the average size of 14±6 nm and 6±2 nm ChR-AuNPs respectively. In vitro anticancer results revealed that the formulated metallic NPs exhibits enhanced cytotoxicity than ChR against treated two different breast carcinoma cell lines (MDA-MB-231 and MDA-MB-468). Furthermore, it was evident that NPs cause cell death via induction of apoptosis. Haemolysis assay with human erythrocytes demonstrates good blood biocompatibility of NPs. Thus, the ChR functionalized metal NPs can be potentially employed as combinational drug-nano platform for breast cancer therapy.

Phytochemical-loaded mesoporous silica nanoparticles for nose-to-brain olfactory drug delivery

“…Similarly for chrysin the hydrodynamic mean diameter increased from 216.8 ± 2.1 nm to 283.5 ± 8.3 nm after loading (P ≤ 0.01) with a statistically significant increase in the PDI from 0.13 ± 0.02 to 0.31 ± 0.11 (P ≤ 0.05) ( Figure 6C) and an associated decrease in zeta potential from -23.9 ± 0.4 mV to -30.8 ± 0.3 mV (P ≤ 0.01) ( Figure 6D). However reports from other chrysin loaded nanoparticle systems indicate a negative zeta potential in the range we detected 86,87 .…”

Diversity Analysis and Bioresource Characterization of Halophilic Bacteria Isolated from a South African Saltpan

“…and reported to protect the retina against light-induced injury [ 72 ]. The compounds chrysin (5,7-dihydroxyflavone) and baicalein (5,6,7-trihydroxyflavone) which were produced by all isolates are known natural flavonoids found in many plant extracts that are commonly used as wound healing, skin protective, anti-tumor and anti-cancer medicines [ 73 , 74 ]. Microbial production of dipeptide has previously been reported [ 19 , 20 ].…”

Synthetic extreme environments: overlooked sources of potential biotechnologically relevant microorganisms

“…The compound 5,7‐dihydroxyflavone (chrysin), which was produced by all isolates, is a known natural flavonoid found in many plant extracts that are commonly used as wound‐healing, skin‐protective and anticancer medicines (Sathishkumar et al ., ). Chrysin is also known for its anti‐inflammatory and antioxidation properties (Engelmann et al ., ).…”