Dietary folic acid addition reduces abdominal fat deposition mediated by alterations in gut microbiota and SCFA production in broilers

Li, Yanli; Yang, Jiantao; Li, Xiaoying; Li, Rui; Wang, Yibin; Huang, Xinhuo; Li, Yingge; Liu, Ruifang; Yang, Xiaojun

doi:10.1016/j.aninu.2022.08.013

Cited by 28 publications

(16 citation statements)

References 43 publications

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“…Through the screening of the correlation network diagram between species, the core species with the highest number of inter-species associations in CON was g_Mogibacterium , a pro-inflammatory bacterium present in the digestive tract of ruminants [ 73 ], and in the group supplemented with oregano essential oil, the core species was g_Ruminococcus , which plays a significant role in the rumen of ruminants in the production of short-chain fatty acids [ 74 ] and the degradation of cellulose [ 75 ]. This suggests that oregano essential oil modulates the structural function of rumen microbes in calves and promotes gastrointestinal digestion.…”

Section: Discussionmentioning

confidence: 99%

Oregano Essential Oil as a Natural Plant Additive Affects Growth Performance and Serum Antibody Levels by Regulating the Rumen Microbiota of Calves

Luo,

Liu,

Cairang

et al. 2024

Animals

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This experiment aimed to investigate whether supplementation of calves with different doses of oregano essential oil (OEO) could promote the development of the gastrointestinal tract and enhance the immune ability of calves by regulating the rumen microbiota. Twenty-four 70-day-old healthy and disease-free Holstein male calves were randomly divided into four groups, with the control group fed a basal diet, and the treatment group provided 4 g, 6 g, and 8 g of oregano essential oil per day in addition to the basal diet. After the 14-day pre-test, a 56-day formal test was conducted. At days 0 and 56 of the standard test period, calves were weighed, the average daily weight gain of calves during the test period was calculated, and serum samples were collected to measure the concentration of immunoglobulins (IgA, IgG, and IgM) in the serum; at day 56 of the formal test period, rumen fluid was collected from the calves, and 16SrRNA was sequenced to analyze changes in the rumen microbiota of the calves. The changes in the rumen microbiota of calves were analyzed by 16SrRNA sequencing. The results of the study showed that (1) OEO supplementation in calves significantly increased end weight and average daily gain (p < 0.05); (2) OEO supplementation in calves significantly increased serum concentrations of immunoglobulins IgA and IgM (p < 0.05); (3) OEO supplementation in calves significantly increased the abundance and diversity of rumen microbial organisms (p < 0.05); (4) OEO supplementation in calves significantly regulates the relative abundance of some species, and biomarkers with significant differences were screened by LEfSe analysis: g_Turicibacter, g_Romboutsia, f_Peptostreptococcaceae, f_Clostridiaceae, g_Clostridium_sensu_stricto_1, o_Clostridiales, g_unclassified_f_Synergistaceae, c_Coriobacteriia, o_Coriobacteriales, f_Atopobiaceae, g_Olsenella, p_Actinobacteriota, g_Defluviitaleaceae_UCG-011, f_Defluviitaleaceae, o_Corynebacteriales, g_Corynebacterium, f_Corynebacteriaceae, g_Shuttleworthia, f_Hungateiclostridiaceae, o_norank_c_Clostridia, g_Saccharofermentans, g_Streptococcus, f_Streptococcaceae, g_unclassified_o_Oscillospirales, and f_unclassified_o_Oscillospirales (p < 0.05, LDA ≥ 3); and (5) OEO supplementation in calves significantly enriched the metabolism of cofactors and vitamins pathway (p < 0.05). (6) Using Superman’s correlation analysis, we screened unclassified_c_Clostridia, Shuttleworthia, and Christensenellaceae_R-7_group, three beneficial strains for calves. (7) Daily supplementation with 8g of OEO significantly affected rumen microbiota regulation in calves.

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Section: Discussionmentioning

confidence: 99%

Oregano Essential Oil as a Natural Plant Additive Affects Growth Performance and Serum Antibody Levels by Regulating the Rumen Microbiota of Calves

Luo,

Liu,

Cairang

et al. 2024

Animals

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“…The dietary arrangements were as follows: birds in CON and FMT group were provided with basal diet (1.3 mg/kg FA); while 13 mg/kg FA was contained in the diet of FA group. The detailed formulation and composition of basal diet were provided in Supplemental Table S1 , which were referred to our previous study ( Liu et al, 2023b ). All birds were obtained from the Xian Dacheng Poultry Industry Co., Ltd. (Xianyang, China).…”

Section: Methodsmentioning

confidence: 99%

“…Cecal chymus form broiler chickens in FA group in our previous study ( Liu et al, 2023b ) were collected and suspended in saline solution. Following agitation and subsequent centrifugation at 800 rpm for 5 min, the fecal microbiota suspension was obtained and stored at −80℃ for future use.…”

Section: Methodsmentioning

confidence: 99%

“…The above studies have collectively underscored the viability of addressing issues related to lipid metabolism in livestock and poultry production by using fecal microbiota transplantation to intervene or reconstruct gut flora. Our previous study revealed that dietary folic acid supplementation reduced abdominal fat deposition, and affected gut microbiota and SCFAs production ( Liu et al, 2023b ), while whether the regulation folic acid regulating of abdominal fat deposition was mediated by gut microbiota was unclear. In the current study, the fecal microbiota from folic acid group prepared before were used to perform FMT, aiming to elucidate the role of gut microbiota in folic acid regulating fat deposition in broiler chickens.…”

Section: Introductionmentioning

confidence: 99%

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Fecal microbiota transplantation revealed the function of folic acid on reducing abdominal fat deposition in broiler chickens mediated by gut microbiota

Liu,

Wang,

et al. 2024

Poultry Science

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“…Several studies have shown that folic acid supplementation can regulate intestinal flora imbalance and metabolic disorders, 41,42 but its effect and mechanism on bone loss are less studied. Through the joint analysis of intestinal flora and metabolites, our study focused on the mechanism of folic acid supplementation on bone loss by improving the body fat rate to the metabolism of LCA in the bile acid synthesis pathway and verified its downstream TGR5 receptor and related signaling pathways.…”

Section: Papermentioning

confidence: 99%

Folic acid supplementation prevents high body fat-induced bone loss through TGR5 signaling pathways

Zhang,

Wei,

Feng

et al. 2024

Food Funct.

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Dietary folic acid addition reduces abdominal fat deposition mediated by alterations in gut microbiota and SCFA production in broilers

Cited by 28 publications

References 43 publications

Oregano Essential Oil as a Natural Plant Additive Affects Growth Performance and Serum Antibody Levels by Regulating the Rumen Microbiota of Calves

Oregano Essential Oil as a Natural Plant Additive Affects Growth Performance and Serum Antibody Levels by Regulating the Rumen Microbiota of Calves

Fecal microbiota transplantation revealed the function of folic acid on reducing abdominal fat deposition in broiler chickens mediated by gut microbiota

Folic acid supplementation prevents high body fat-induced bone loss through TGR5 signaling pathways

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