Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption

Gonzalez‐Vicente, Agustin; Cabral, Pablo D.; Hong, Nancy J.; Asirwatham, Jessica; Yang, Nianxin; Berthiaume, Jessica M.; Dominici, Fernando Pablo; Garvin, Jeffrey L.

doi:10.3390/nu9080885

Cited by 19 publications

(43 citation statements)

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“…Dietary Treatment: After arrival at our animal facility, all rats were fed a purified diet (Test-Diet #9GDV; St. Louis, MO, USA). This diet was designed to match the components of Test-Diet #5876 used in our previous study [ 38 ] with the exception of having 700 mEq Na + /kg (~16 g of Na + /kg) instead of 100 mEq Na + /kg. Rats were randomly assigned to two experimental groups: one receiving tap water; and the other drinking 20% fructose.…”

Section: Methodsmentioning

confidence: 99%

“…Proximal tubule suspension: proximal tubule suspensions were generated using methods similar to those we used before [ 38 ]. Briefly, rats were anesthetized and received 2 units of heparin by intraperitoneal injection.…”

Section: Methodsmentioning

confidence: 99%

“…Interfering with the effects of Ang II blunts the increase in blood pressure in fructose-fed rats [ 32 , 33 , 34 , 35 , 36 , 37 ]. Furthermore, we reported that dietary fructose in the absence of a high-salt diet enhances the sensitivity of proximal nephron Na + transport to the stimulatory effect of Ang II [ 38 ]. Together these data support a role for both Ang II and proximal tubules in salt-sensitive hypertension induced by fructose but this has not been studied.…”

Section: Introductionmentioning

confidence: 99%

“…Activation of protein kinase C by Ang II blunts the effects of ANP [ 47 , 48 ] and acute fructose treatment enhances the effects of Ang II via protein kinase C when rats are fed a low-salt diet [ 49 ]. Given that dietary fructose sensitizes proximal tubule Na + reabsorption to Ang II [ 38 ], one might expect that the effect of ANP on proximal tubule transport would be blunted by dietary fructose when rats are on a high-salt diet. However, it is unknown whether the response of proximal tubules to ANP is altered during fructose-induced salt-sensitive hypertension.…”

Section: Introductionmentioning

confidence: 99%

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Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets

et al. 2018

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Dietary fructose causes salt-sensitive hypertension. Proximal tubules (PTs) reabsorb 70% of the filtered NaCl. Angiotensin II (Ang II), atrial natriuretic peptide (ANP) and norepinephrine (NE) regulate this process. Although Ang II signaling blockade ameliorates fructose-induced salt-sensitive hypertension, basal PT Na+ reabsorption and its sensitivity to the aforementioned factors have not been studied in this model. We hypothesized consuming fructose with a high-salt diet selectively enhances the sensitivity of PT transport to Ang II. We investigated the effects of Ang II, ANP and NE on PT Na reabsorption in rats fed a high-salt diet drinking tap water (HS) or 20% fructose (HS-FRU). Oxygen consumption (QO2) was used as a measure of all ATP-dependent transport processes. Na+/K+-ATPase and Na+/H+-exchange (NHE) activities were studied because they represent primary apical and basolateral transporters in this segment. The effect of 10−12 mol/L Ang II in QO2 by PTs from HS-FRU was larger than HS (p < 0.02; n = 7). In PTs from HS-FRU 10−12 mol/L Ang II stimulated NHE activity by 2.6 ± 0.7 arbitrary fluorescence units/s (p < 0.01; n = 5) but not in those from HS. The stimulatory effect of Ang II on PT Na+/K+-ATPase activity was not affected by HS-FRU. Responses of QO2 and NHE activity to ANP did not differ between groups. The response of QO2 to NE was unaltered by HS-FRU. We concluded that the sensitivity of PT Na+ reabsorption specifically to Ang II is enhanced by HS-FRU. This maintains high rates of transport even in the presence of low concentrations of the peptide, and likely contributes to the hypertension.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets

et al. 2018

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“…The adrenocorticotrophic hormone, growth hormone, prolactin, lipid peroxidase, and C-reactive protein levels were measured using the radio immunological method. The lactic dehydrogenase, lactic acid, malonaldehyde, catalase, glutathione peroxidase, superoxide dismutase, sodium-potassium ATPase (NA + /K + -ATPase), and urea nitrogen levels were measured using the blood biochemical method [24][25][26]. Intra-and inter-assay coefficients of variation were calculated by analyzing standards in each assay.…”

Section: Biochemical Indicators Determination and Analysismentioning

confidence: 99%

Heat Stress Impairs the Physiological Responses and Regulates Genes Coding for Extracellular Exosomal Proteins in Rat

Dou

Khan

et al. 2020

Genes

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Heat stress (HS) is challenging in humans and animals as it is a complicated regulatory mechanism. This prompted us to characterize the physiological and molecular responses of a HS-animal model. In this study, a rat model system was developed by using three temperature treatments (40°C, 42°C, and 43°C) and sixteen biochemical indicators in blood at 42°C for 30 min (H30), 60 min (H60), and 120 min (H120). In addition, transcriptomic profiling was carried out in H120-rats' blood, liver, and adrenal gland samples for detection of the genes of interest. Our findings demonstrated that the adrenocorticotropic hormone, catalase, prolactin, growth hormone, and lactic acid have significant spatiotemporal variation in the H120-rats as compared with the control. Furthermore, through transcriptomic screening, we documented a high ratio of differentially expressed genes (DEGs) in adrenal glands, liver, and blood, respectively. Among them, Nup153, Plxnb2, Stx7, Hspa9, Chordc1, Pde4d, Gm2α, and Rnf125 were associated with the regulation of HS and immune response processes. Notably, 36 and 314 of DEGs in blood and adrenal glands were detected in the composition of the extracellular exosome, respectively. Furthermore, the correlation analysis between gene transcripts and biochemical indicator levels identified the Lgals3, S1006, Fn1, F2, and Kng1l1 as key candidate genes for HS encoding extracellular exosomal proteins. On the basis of our results, it was concluded that the current rat model provides a molecular basis for future research in HS resistance in humans and livestock.Genes 2020, 11, 306 2 of 23 (e.g., crossbreeding), have not achieved long-lasting, cumulative and significant effects [9]. Thus, further molecular mechanisms and physiological consequences of HS using an animal model are highly recommended.Previous studies have shown HS-induced physiological and biochemical variations in many animals, such as rats [10,11], cows [12], and chickens [13]. The HS causes an imbalance of glucocorticoids, the adrenocorticotropic hormone, the growth hormone, and the norepinephrine hormone, resulting in detrimental metabolic changes [10,14,15]. Furthermore, a set of proteins involved in the antioxidant stress response and inflammatory response, including catalase, glutathione peroxidase, and C-reactive protein were affected by HS [16][17][18][19]. Recently, microarray and next-generation sequencing studies have displayed widespread changes in gene expression in response to HS, the exploration of useful indicators to predict and prevent HS in animals has yet to be defined.In the context of the critical effects of HS on human and animal health, the current study was designed to explore the physiological, biochemical, and transcriptomic responses to HS in Sprague Dawley (SD) rats. The current research targets the key biomarkers that are sensitive to HS. In addition, the altered expression of genes related to HS response was detected by performing transcriptomic screening in blood, liver, and adrenal gland tissues post HS. Collectively...

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Renal Tubular Handling of Glucose and Fructose in Health and Disease

Vallon

Nakagawa

2021

Comprehensive Physiology

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Dietary Fructose Enhances the Ability of Low Concentrations of Angiotensin II to Stimulate Proximal Tubule Na+ Reabsorption

Cited by 19 publications

References 73 publications

Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets

Dietary Fructose Increases the Sensitivity of Proximal Tubules to Angiotensin II in Rats Fed High-Salt Diets

Heat Stress Impairs the Physiological Responses and Regulates Genes Coding for Extracellular Exosomal Proteins in Rat

Renal Tubular Handling of Glucose and Fructose in Health and Disease

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