Dietary glycated protein modulates the colonic microbiota towards a more detrimental composition in ulcerative colitis patients and non-ulcerative colitis subjects

Abstract

Aim: To investigate the effect of native, heated and glycated bovine serum albumin (BSA) on the ulcerative colitis (UC) and non‐UC colonic microbiota in vitro. Methods and Results: Continuous flow culture (CFC) models of the human colonic microbiota inoculated with faeces from UC and non‐UC volunteers were maintained on BSA as growth substrate. Changes in bacterial populations and short‐chain fatty acids were determined. UC and non‐UC microbiota differed significantly in microbial populations, with elevated numbers of sulfate‐reducing bacteria (SRB) and clostridia in the microbiota from UC patients. Compared with native BSA, glycated BSA modulated the gut microbiota of UC patients in vitro towards a more detrimental community structure with significant increases in putatively harmful bacteria (clostridia, bacteroides and SRB; P < 0·009) and decreases in dominant and putatively beneficial bacterial groups (eubacteria and bifidobacteria; P < 0·0004). The levels of beneficial short‐chain fatty acids were significantly decreased by heated or glycated BSA, but were increased significantly by native BSA. Conclusion: The UC colonic microbiota maintained in CFC was significantly modified by glycated BSA. Significance and Impact of the Study: Results suggest that dietary glycated protein may impact upon the composition and activity of the colonic microbiota, an important environmental variable in UC.

A Post-Genomic View of the Ecophysiology, Catabolism and Biotechnological Relevance of Sulphate-Reducing Prokaryotes

“…The known detrimental genotoxic (Attene- Ramos, Wagner, Plewa, & Gaskins, 2006), T-cell activation enhancing (Miller & Richardson, 2012) and colonic microbiome shaping effects of produced hydrogen sulphide may be promoted by its unhindered diffusion across lipid membranes (Mathai et al, 2009) and may be indirectly influenced by the dietary effects on the abundance of SRP (e.g. Mills et al, 2008). Another member of the intestinal flora closely related to SRP is B. wadsworthia.…”

‘The way to a man's heart is through his gut microbiota’ – dietary pro- and prebiotics for the management of cardiovascular risk

“…Interestingly, Fernandes et al (104) have recently confirmed that methane production and carriage of detectable Archaea (mainly methanogens in human subjects) are inversely related to BMI and that methanogenesis appears to allow more efficient production and absorption of SCFA from dietary fibre fermentation. While the ecological processes regulating the relative abundance of gut SRB and methanogens are not fully understood, ingestion of fermentable fibre increases methanogenesis in CH 4 -producing subjects, while sulphate or dietary protein appear to stimulate the numbers of SRB and presumably their metabolic activities within the gut microbiota (105)(106)(107)(108)(109) .…”

Glycation: Implication in Perceived Age and Dermatology

“…In addition, AGEs also infl uence the normal gut fl ora (95), reducing the amounts of "good bacteria" (96) and compromising nutrient's and phytochemicals' absorbtion.…”