Dietary gossypol suppressed postprandial TOR signaling and elevated ER stress pathways in turbot (<i>Scophthalmus maximus</i> L.)

Bian, Fuyun; Jiang, Haowen; Man, Mingsan; Mai, Kangsen; Zhou, Huihui; Xu, Wei; He, Gang

doi:10.1152/ajpendo.00285.2016

Cited by 37 publications

(17 citation statements)

References 71 publications

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“…The following antibodies were used: antibodies against protein kinase B (AKT, 9272), phospho-AKT (Ser473, 9271) and β-tubulin (2146) were purchased from Cell Signaling Technology. All these antibodies were developed using antigenic regions completely conserved in fish, and could be used in scophthalmidae turbot 90 . The Western bands were quantified using NIH Image 1.63 software.…”

Section: Methodsmentioning

confidence: 99%

Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus

Deng

Pan

Liu

et al. 2018

Sci Rep

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Carnivorous fish is thought to be high-glucose intolerance. But the reasons were still unclear. The aim of the present study is to investigate the effects of high level of dietary carbohydrate on the survival, growth and immune responses of Paralichthys olivaceus, and the underlying molecular mechanism related to the immune and glucose metabolism. P. olivaceus were fed with 8%, 16% and 24% of dietary carbohydrate for 10 weeks, respectively. After that, a glucose tolerance test (GTT) was conducted. Results showed that excessive (24%) dietary carbohydrate significantly decreased the growth and glucose tolerance ability according to the GTT. It significantly increased hepatic NADPH oxidase activity and malondialdehyde content and serum contents of IL-6 and advanced glycation end products. The expressions of glucose transport-relevant genes in liver and the content of related hormones in serum were analyzed. In conclusion, it was confirmed that IL-6 increased the expression of suppressor of cytokine signaling 3 (SOCS3) and regulated the downstream targets of PI3K-AKT mediated signal transduction, and then downregulated the glucose transporter 2 activity in liver of P. olivaceus fed diet with excessive carbohydrate level. It was suggested that SOCS3 served as a bridge between immune response and glucose metabolism in P. olivaceus.

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Section: Methodsmentioning

confidence: 99%

Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus

Deng

Pan

Liu

et al. 2018

Sci Rep

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“…no. 373702) antibodies were from Santa Cruz Biotechnology Inc. All these antibodies were developed using antigenic regions completely conserved in turbot, and many had been successfully used in turbot as reported before ( 34 , 44 , 51 ). All experiments were repeated at least three times.…”

Section: Methodsmentioning

confidence: 99%

The Mitotic and Metabolic Effects of Phosphatidic Acid in the Primary Muscle Cells of Turbot (Scophthalmus maximus)

Wang

Zhou

et al. 2018

Front. Endocrinol.

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Searching for nutraceuticals and understanding the underlying mechanism that promote fish growth is at high demand for aquaculture industry. In this study, the modulatory effects of soy phosphatidic acids (PA) on cell proliferation, nutrient sensing, and metabolic pathways were systematically examined in primary muscle cells of turbot (Scophthalmus maximus). PA was found to stimulate cell proliferation and promote G1/S phase transition through activation of target of rapamycin signaling pathway. The expression of myogenic regulatory factors, including myoD and follistatin, was upregulated, while that of myogenin and myostatin was downregulated by PA. Furthermore, PA increased intracellular free amino acid levels and enhanced protein synthesis, lipogenesis, and glycolysis, while suppressed amino acid degradation and lipolysis. PA also was found to increased cellular energy production through stimulated tricarboxylic acid cycle and oxidative phosphorylation. Our results identified PA as a potential nutraceutical that stimulates muscle cell proliferation and anabolism in fish.

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“…Since the serum biochemical indices and the level of phospho‐AMPKα of fish were significantly affected by refeeding time according to our previous results of preliminary experiment (Bian et al, ; Tian et al, ), samples were collected at four time points to test the validity and the time of peak value of these indices. The remained 36 fish of each treatment were mixed and reassigned equally to four tanks in the same system described above.…”

Section: Methodsmentioning

confidence: 99%

“…no. 2146) were purchased from Cell Signaling Technology Inc. Antibody directed against SREBP‐1 (SC‐13551) was purchased from Santa Cruz Biotechnology Inc. All these antibodies have been used successfully with fish in the previous studies (Bian et al, ; Librán‐Pérez et al, ; Tian et al, ; Wang et al, ), and refer to the relevant website for detailed information about the antibodies.…”

Section: Methodsmentioning

confidence: 99%

AMPK activation by dietary AICAR affects the growth performance and glucose and lipid metabolism in juvenile grass carp

et al. 2019

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Fish are poor users of dietary carbohydrates and considered to be glucose intolerant, and activating AMP‐activated protein kinase (AMPK) by 5‐aminoimidazole‐4‐carboxamide ribonucleoside (AICAR) has been shown to stimulate glucose uptake and utilization in skeletal muscle cells of rainbow trout. However, the effects of activating AMPK by dietary AICAR in the piscine model remain largely unexplored. In the present study, grass carp were fed purified diets including AICAR at a level of 0 or 200 mg/kg diet to chronically activate AMPK for 8 weeks, and the dietary effects on phenotypic and molecular responses related to glucose and lipid metabolism were examined. The results showed that activating AMPK by dietary AICAR significantly reduced fish growth performance, serum glucose and triacylglycerol contents, and liver lipid contents, while it increased hepatic glycogen contents (p < 0.05). The survival was not influenced by AICAR treatment (p > 0.05). The results of AMPK signalling pathway in both the liver and muscle show that dietary AICAR increased the phosphorylation of AMPKα and acetyl‐CoA carboxylase (ACC; p < 0.05), while inhibiting the phosphorylation of sterol regulatory element‐binding transcription factor‐1(SREBP‐1) and target of rapamycin (p < 0.05). The activation of AMPK reduced the mRNA expression of lipogenesis enzymes in the liver and muscle (p < 0.05) and increased mRNA expression of gluconeogenesis enzymes in the liver and glycolysis enzymes in the heart (p < 0.05). Our results demonstrate that activating AMPK by AICAR reduces serum glucose and inhibits activities of ACC and SREBP‐1 to reduce lipogenesis in the liver and muscle, and AMPK could be used as an active target for its metabolic syndromes of grass carp.

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Dietary gossypol suppressed postprandial TOR signaling and elevated ER stress pathways in turbot (Scophthalmus maximus L.)

Cited by 37 publications

References 71 publications

Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus

Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus

The Mitotic and Metabolic Effects of Phosphatidic Acid in the Primary Muscle Cells of Turbot (Scophthalmus maximus)

AMPK activation by dietary AICAR affects the growth performance and glucose and lipid metabolism in juvenile grass carp

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