Dietary hydrolyzed wheat gluten supplementation ameliorated intestinal barrier dysfunctions of broilers challenged with Escherichia coli O78

Abstract

This study aimed to investigate a protective effect of hydrolyzed wheat gluten ( HWG ) on Escherichia coli ( E. coli )-induced intestinal barrier dysfunctions in broilers. Broilers fed a basal diet unsupplemented or supplemented with HWG (0.5% or 1%) were intraperitoneally injected with either E. coli O78 suspension (10 8 CFU/mL) or equal volume of vehicle on d 18 of age. Blood and tissue samples were collected 3rd d post infection. The results showed that E. coli -infection increased immune-organ indexes of spleen and thymus, enhanced serum diamine oxidase ( DAO ) level, impaired ileal villus structure and reduced tight junction mRNA levels ( Occludin, Claudin-1, ZO-1, P < 0.05), while increased mRNA levels of inflammatory cytokines ( TNF-α, IFN-γ, IL-1β, IL-6, IL-8 ) and TLR4 in the ileum of broilers ( P < 0.05). The effects of E. coli O78 challenge on organ indexes of spleen and thymus, serum DAO level, mRNA levels of tight junctions were alleviated by 1% HWG supplementation, the upregulations of IL-1β and TLR4 were prevented by 0.5% HWG supplementation ( P < 0.05). In addition, increased IFN-γ of E. coli -infected broilers was abrogated by 0.5% or 1% HWG supplementation ( P < 0.05). In summary, dietary HWG supplementation ameliorated intestinal barrier dysfunctions triggered by E. coli -infection in the ileum of broilers. HWG supplementation might be a nutritional strategy to improve the intestinal mucosal barrier function of broilers.

Artemisia annua L. polysaccharide improves the growth performance and intestinal barrier function of broilers challenged with Escherichia coli

“…In the current study, oral administration of E. coli significantly increased the mRNA level of TLR4 and MyD88 in jejunum, and dietary supplementation of AAP alleviated the upregulation of mRNA expression level. These results are a line with those found by Wu et al (2022b) , who reported that dietary hydrolyzed wheat gluten supplementation down-regulated the elevation of ileal TLR4 mRNA level caused by E. coli O78-challenge in broilers. It might be related to the anti-inflammatory activity of arabinose, galactose and fucoidan in plant-derived polysaccharides ( Wang et al, 2023 ).…”

“…Furthermore, Kumari et al (2023) discovered that added drinking water with Aloe vera leaf extract could mitigate the detrimental effects of E. coli -challenge on broilers by enhancing antioxidant level and bolstering cellular immune response. In addition, studies showed that dietary hydrolyzed wheat gluten supplementation ameliorated intestinal barrier dysfunction of broilers challenged with E. coli O78 ( Wu et al, 2022b ).…”

Dietary ethylenediamine dihydroiodide mitigated Escherichia coli O78-induced immune and intestinal damage of ducks via suppression of NF-κB signal

“…In this study, APEC challenge resulted in a significant reduction in the thymus index and bursa index in meat ducks, which was consistent with the findings of Zhang et al (2020) on meat chickens. However, in conflict with this, Wu et al (2021) found a significant increase in the spleen index in broilers after APEC challenge, which may be due to congestion and swelling of the immune organs during the short period of time caused by APEC challenge, thus increasing the index of the immune organs. Unfortunately, there were few public data about the effects of iodine on immune organ index of meat ducks, which remains to be further investigated.…”

Effects of fecal microbiota transplantation and fecal virome transplantation on LPS-induced intestinal injury in broilers

“…IFN-γ is a key molecule in the promotion of cellular immunity, and it is capable of promoting inflammation and inducing an antiviral state through the coordinated integration of cytokines and signals from pattern-recognition receptors, such as IL-4 , LPS, and type I interferon, thereby enhancing the resistant ability to pathogens ( Kak et al, 2018 ). Dietary addition of hydrolyzed wheat gluten ( Wu et al, 2022 ) or dietary oregano essential oils ( Feng et al, 2021 ), etc. could significantly reduce broiler ileal IFN-γ mRNA expression.…”