Dietarytrans-10,cis-12-conjugated linoleic acid alters fatty acid metabolism and microbiota composition in mice

Abstract

The main aim of the present study was to investigate the effects of dietary trans-10, cis-12-conjugated linoleic acid (t10c12-CLA) on intestinal microbiota composition and SCFA production. C57BL/6 mice (n 8 per group) were fed a standard diet either supplemented with t10c12-CLA (0·5 %, w/w) (intervention) or with no supplementation (control), daily for 8 weeks. Metabolic markers (serum glucose, leptin, insulin and TAG, and liver TAG) were assessed by ELISA commercial kits, tissue long-chain fatty acids and caecal SCFA by GC, and microbial composition by 16S rRNA pyrosequencing. Dietary t10c12-CLA significantly decreased visceral fat mass (P,0·001), but did not affect body weight (intervention), when compared with no supplementation (control). Additionally, lipid mass and composition were affected by t10c12-CLA intake. Caecal acetate, propionate and isobutyrate concentrations were higher (P, 0·05) in the t10c12-CLAsupplemented group than in the control group. The analysis of the microbiota composition following 8 weeks of t10c12-CLA supplementation revealed lower proportions of Firmicutes (P¼ 0·003) and higher proportions of Bacteroidetes (P¼ 0·027) compared with no supplementation. Furthermore, t10c12-CLA supplementation for 8 weeks significantly altered the gut microbiota composition, harbouring higher proportions of Bacteroidetes, including Porphyromonadaceae bacteria previously linked with negative effects on lipid metabolism and induction of hepatic steatosis. These results indicate that the mechanism of dietary t10c12-CLA on lipid metabolism in mice may be, at least, partially mediated by alterations in gut microbiota composition and functionality.

Temporal bacterial and metabolic development of the preterm gut reveals specific signatures in health and disease

“…Based on the findings here, the progression toward NEC may be detected between 1–2 weeks prior to current clinical diagnosis, which is consistent with the previous data [22]. Such findings require validation in large multicentre studies, but the finding that 4/5 metabolites most associated with NEC were involved in linoleate metabolism is intriguing given that trans -10, cis -12-conjugated linoleic acid ( t 10 c 12-CLA) negatively impacts on lipid metabolism, mediated by alterations in gut microbiome [23]. As more metabolomic investigations are performed, the mechanism of linoleate metabolism in an inflammatory-mediated damage may be better elucidated, but the results of this study support the concept that it is, at least in part, mediated by the microbiome.…”

FODMAPs alter symptoms and the metabolome of patients with IBS: a randomised controlled trial

“…Interestingly, despite a decrease in Bifidobacteria relative abundance after the low FODMAP diet, Actinobacteria richness and diversity was still higher in these patients in comparison to those on the high FODMAP diet. However, we also observed that the shift caused by the low FODMAP diet to the microbial community left a niche open to potentially harmful bacteria, such as Porphyromonadaceae,42 43 which we found to be strongly associated with urinary histamine levels.…”

The Microbiome and Biomarkers for Necrotizing Enterocolitis: Are We Any Closer to Prediction?

“…The study was expanded to additional time points before and after the onset of NEC and they detected a temporal profile of these metabolites that were significantly different in infants with NEC versus controls. These findings are particularly intriguing, in light of a previous report that showed changes in the gut microbiome of mice after they were exposed to dietary trans-10, cis-12-conjugated linoleic acid (69). A new approach utilized existing metabolomic data from newborn screening and identified multiple acylcarnitines that differed significantly in preterm infants who subsequently developed NEC (70).…”