Dietary Intake of Flaxseed Oil since Early Stages of Life Promotes Femur Quality in Male Rats

Pereira, Aline D'Avila; Ribeiro, Danielle Cavalcante; Cardoso, Letícia Monteiro da Fonseca; Ribeiro, Guilherme; Quintes, Bruna da Costa Rodrigues; Boueri, Bianca Ferolla da Camara; Costa, Nathalia da Silva; Chagas, Mauricio Alves; Silva, Eduardo Moreira da; Costa, Carlos Alberto Soares da; Velarde, Luís Guillermo Coca; Boaventura, Gilson Teles

doi:10.1080/07315724.2021.1912673

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…In addition, SDG could increase the weight of the femur, the content of serum calcium, serum phosphorus and bone calcium [ 38 ]. Bone histopathology studies showed that SDG could increase Tb.N, Tb.Th, BV/TV, increase the mean bone cortical thickness and reduce the mean Tb.Sp for the treatment of OP [ 39 ]. Therefore, we performed bilateral ovariectomy in SD rats [ 28 ] to establish an animal model of PMOP that reduce the estrogen levels.…”

Section: Discussionmentioning

confidence: 99%

Secoisolariciresinol diglucoside regulates estrogen receptor expression to ameliorate OVX-induced osteoporosis

Chen,

Hong

et al. 2023

J Orthop Surg Res

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Objective Secoisolariciresinol diglucoside (SDG) is a phytoestrogen that has been reported to improve postmenopausal osteoporosis (PMOP) caused by estrogen deficiency. In our work, we aimed to investigate the mechanism of SDG in regulating the expressions of ERs on PMOP model rats. Methods Ovariectomization (OVX) was used to establish PMOP model in rats. The experiment was allocated to Sham, OVX, SDG and raloxifene (RLX) groups. After 12-week treatment, micro-CT was used to detect the transverse section of bone. Hematoxylin and Eosin staining and Safranine O-Fast Green staining were supplied to detect the femur pathological morphology of rats. Estradiol (E2), interleukin-6 (IL-6), bone formation and bone catabolism indexes in serum were detected using ELISA. Alkaline phosphatase (ALP) staining was used to detect the osteogenic ability of chondrocytes. Immunohistochemistry and Western blot were applied to detect the protein expressions of estrogen receptors (ERs) in the femur of rats. Results Compared with the OVX group, micro-CT results showed SDG could lessen the injury of bone and improve femoral parameters, including bone mineral content (BMC) and bone mineral density (BMD). Pathological results showed SDG could reduce pathological injury of femur in OVX rats. Meanwhile, SDG decreased the level of IL-6 and regulated bone formation and bone catabolism indexes. Besides, SDG increased the level of E2 and conversed OVX-induced decreased the expression of ERα and ERβ. Conclusion The treatment elicited by SDG in OVX rats was due to the reduction of injury and inflammation and improvement of bone formation index, via regulating the expression of E2 and ERs.

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Section: Discussionmentioning

confidence: 99%

Secoisolariciresinol diglucoside regulates estrogen receptor expression to ameliorate OVX-induced osteoporosis

Chen,

Hong

et al. 2023

J Orthop Surg Res

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“…Forty grams of flaxseed per day for 12 months resulted in no significant change in bone mineral density in menopausal women [ 89 ]. However, flaxseed oil [ 90 , 91 ] or ground flaxseed [ 92 ] have significant beneficial effects on bone composition, mineral density and content, area, breaking strength and maximal force in young developing rats [ 90 , 91 , 92 , 93 ]. This is likely due to the ALA and omega-3 fatty acid content in flaxseed.…”

Section: Bone Density and Strengthmentioning

confidence: 99%

“…This is likely due to the ALA and omega-3 fatty acid content in flaxseed. Even delivering the flaxseed to the mothers during lactation was sufficient to induce these positive effects on bone development in the weaning pups [ 90 , 92 ]. Furthermore, in hemodialysis patients where bone disorders are common, augmentation of the diet with flaxseed oil reduced bone resorption in these patients [ 94 ].…”

Section: Bone Density and Strengthmentioning

confidence: 99%

Physiological and Pathological Considerations for the Use of Flaxseed as a Therapeutic Dietary Strategy

Hirst,

Dibrov,

Hirst

et al. 2023

Rev. Cardiovasc. Med.

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The inclusion of flaxseed in the diet may have a great number of potential benefits for the well-being of both healthy individuals and those challenged by disease conditions as well. With an increase in the number and quality of studies focused on the physiological and pathophysiological effects of dietary flaxseed, our knowledge concerning the rationale for the inclusion of flaxseed in our diet has become more convincing and stronger. The purpose of this review is threefold. First, the review will comprehensively document the evidence supporting the value of dietary flaxseed to improve bodily health in both normal and disease conditions. Second, this review will identify the mechanisms of action responsible for these effects. Finally, this article will review practical aspects relevant to the inclusion of flaxseed in the diet. Briefly, supplementing the diet with flaxseed has beneficial effects on the treatment and/or prevention of different kinds of cardiovascular disease (hypertension, ischemic heart disease, myocardial infarcts, atherosclerosis), non-alcoholic fatty liver disease, breast cancer, bone strength, menopause, diabetes, and wound healing. Although some controversy exists on the component within flaxseed that provides these beneficial actions, it is likely that the rich content of the omega-3 fatty acid, alpha linolenic acid, is primarily responsible for the majority of these biological effects. It is concluded that the constantly expanding evidence in support of the inclusion of flaxseed in our daily diet to provide significant health benefits strongly encourages the initiation of additional work on dietary flaxseed in order to both confirm past findings as well as to further advance our knowledge regarding the important biological actions of dietary flaxseed.

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Development of polyvinyl alcohol nanofiber scaffolds loaded with flaxseed extract for bone regeneration: phytochemicals, cell proliferation, adhesion, and osteogenic gene expression

Abdelaziz,

Nageh,

Abdalla

et al. 2024

Front. Chem.

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Introduction: Bone tissue engineering seeks innovative materials that support cell growth and regeneration. Electrospun nanofibers, with their high surface area and tunable properties, serve as promising scaffolds. This study explores the incorporation of flaxseed extract, rich in polyphenolic compounds, into polyvinyl alcohol (PVA) nanofibers to improve their application in bone tissue engineering.Methods: High-performance liquid chromatography (HPLC) identified ten key compounds in flaxseed extract, including polyphenolic acids and flavonoids. PVA nanofibers were fabricated with 30 wt.% flaxseed extract (P70/E30) via electrospinning. We optimized characteristics like diameter, hydrophilicity, swelling behavior, and hydrolytic degradation. MG-63 osteoblast cultures were used to assess scaffold efficacy through cell adhesion, proliferation, viability (MTT assay), and differentiation. RT-qPCR measured expression of osteogenic genes RUNX2, COL1A1, and OCN.Results: Flaxseed extract increased nanofiber diameter from 252 nm (pure PVA) to 435 nm (P70/E30). P70/E30 nanofibers showed higher cell viability (102.6% vs. 74.5% for pure PVA), although adhesion decreased (151 vs. 206 cells/section). Notably, P70/E30 enhanced osteoblast differentiation, significantly upregulating RUNX2, COL1A1, and OCN genes.Discussion: Flaxseed extract incorporation into PVA nanofibers enhances bone tissue engineering by boosting osteoblast proliferation and differentiation, despite reduced adhesion. These properties suggest P70/E30’s potential for regenerative medicine, emphasizing scaffold optimization for biomedical applications.

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Dietary Intake of Flaxseed Oil since Early Stages of Life Promotes Femur Quality in Male Rats

Cited by 3 publications

References 23 publications

Secoisolariciresinol diglucoside regulates estrogen receptor expression to ameliorate OVX-induced osteoporosis

Secoisolariciresinol diglucoside regulates estrogen receptor expression to ameliorate OVX-induced osteoporosis

Physiological and Pathological Considerations for the Use of Flaxseed as a Therapeutic Dietary Strategy

Development of polyvinyl alcohol nanofiber scaffolds loaded with flaxseed extract for bone regeneration: phytochemicals, cell proliferation, adhesion, and osteogenic gene expression

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