Vitamin D has drawn attention as a significant nutrient to prevent pregnancy complications and health problems in offspring (1, 2). For instance, maternal vitamin D deficiency has been speculated to cause endothelial dysfunction, a key feature of preeclampsia, through a proinflammatory response and increase in oxidative stress (3). Its deficiency also leads to low birth size through the inhibition of bone mineral accrual of the fetus (4).Maintaining maternal circulating 25-hydroxyvitamin D (25(OH)D) concentrations above 30.0 ng/mL is recommended (5). However, the high ratio of severe vitamin D deficiency (25(OH)D,10.0 ng/mL) in pregnant women is a significant concern in many countries (6-8). In Japan, two studies showed that the mean serum 25(OH)D concentrations during pregnancy were only 9.5-14.5 ng/mL (9, 10). The concentrations were especially lower in winter. To improve the vitamin D status of pregnant Japanese women, identification of populations at a high risk of vitamin D deficiency and implementation of appropriate intervention are necessary. In general, vitamin D status depends on vitamin D intake (vitamin D21D3) and vitamin D3 production in the skin through exposure to sunlight (11). Although vitamin D status was reported to be affected by age, body mass index (BMI), physical activity, and season (12-15), factors associated with vitamin D status vary with the target population and latitude. Among pregnant Australian women, low serum 25(OH)D concentrations were significantly predicted by non-Caucasian, high BMI, non-use of vitamin D supplements, and winter season (14). Among non-pregnant Japanese women, young age, low dietary vitamin D intake, low walk counts, and long sedentary activity time correlated with low serum 25(OH)D concentrations (16,17). The walk counts and sedentary activity time were presumed to reflect the amount of exposure to sunlight. However, it remains unclear which factors are associated with vitamin D status in pregnant Japanese women, the extent to which those factors are associated with vitamin D status, and whether the factors vary with the season.The objective of the present study was to examine demographic and lifestyle factors associated with