Dietary interventions in mice affect oxidative stress and gene expression of the Prlr and Esr1 in the adipose tissue and hypothalamus of dams and their offspring

Gauthier, Mariana Fraga; Andrade, Andressa Alves de; Fisch, Joana; Feistauer, Vanessa; Morás, Ana Moira; Reinhardt, Luiza Steffens; Moura, Dinara Jaqueline; Almeida, Silvana; Guedes, Renata Padilha; Moura, Ana Carolina de

doi:10.1007/s13105-021-00862-5

Cited by 7 publications

(2 citation statements)

References 39 publications

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“…S-PRLR differs from L-PRLR in that it has a 39-base pair inserted at the beginning of the cytoplasmic domain and two consecutive intra-frame terminating codons at its 3 end [15]. Oxidative stress in offspring mice was found to be associated with an increased expression of PRLR in the hypothalamus and adipose tissue due to the restrictive or hypercaloric diets of maternal mice [49]. Dandawate et al found that penfluridol could block the expression of PRLR in pancreatic cancer cells leads to a reduction in cell proliferation, induction of autophagy, and deceleration of xenograft tumour growth in mice [50].…”

Section: Prl Regulated Oxidative Stress Autophagy and Apoptosis By Bi...mentioning

confidence: 97%

High Prolactin Concentration Induces Ovarian Granulosa Cell Oxidative Stress, Leading to Apoptosis Mediated by L-PRLR and S-PRLR

Yang,

Duan,

Zhang

et al. 2023

IJMS

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High prolactin (PRL) concentration has been shown to induce the apoptosis of ovine ovarian granulosa cells (GCs), but the underlying mechanisms are unclear. This study aimed to investigate the mechanism of apoptosis induced by high PRL concentration in GCs. Trial 1: The optimal concentration of glutathion was determined according to the detected cell proliferation. The results showed that the optimal glutathione concentration was 5 μmol/mL. Trial 2: 500 ng/mL PRL was chosen as the high PRL concentration. The GCs were treated with 0 ng/mL PRL (C group), 500 ng/mL PRL (P group) or 500 ng/mL PRL, and 5 μmol/mL glutathione (P-GSH group). The results indicated that the mitochondrial respiratory chain complex (MRCC) I–V, ATP production, total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and thioredoxin peroxidase (TPx) in the C group were higher than those in the P group (p < 0.05), while they were lower than those in the P-GSH group (p < 0.05). Compared to the C group, the P group exhibited elevated levels of reactive oxygen species (ROS) and apoptosis (p < 0.05) and increased expression of ATG7 and ATG5 (p < 0.05). However, MRCC I–V, ATP, SOD, A-TOC, TPx, ROS, and apoptosis were decreased after the addition of glutathione (p < 0.05). The knockdown of either L-PRLR or S-PRLR in P group GCs resulted in a significant reduction (p < 0.05) in MRCC I–V, ATP, T-AOC, SOD and TPx, while the overexpression of either receptor showed an opposite trend (p < 0.05). Our findings suggest that high PRL concentrations induce apoptotic cell death in ovine ovarian GCs by downregulating L-PRLR and S-PRLR, activating oxidative stress and autophagic pathways.

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Section: Prl Regulated Oxidative Stress Autophagy and Apoptosis By Bi...mentioning

confidence: 97%

High Prolactin Concentration Induces Ovarian Granulosa Cell Oxidative Stress, Leading to Apoptosis Mediated by L-PRLR and S-PRLR

Yang,

Duan,

Zhang

et al. 2023

IJMS

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“…Bisphenol A exposure can promote ovarian ROS/RNS synthesis and lipid peroxidation and then changes the expression of the estrogen receptor gene, ultimately affecting ovarian and reproductive health in organisms [12]. Dietary interventions in mice affect oxidative stress and gene expression of Esr1 in the adipose tissue and hypothalamus of dams and their offspring [13]. The testis is vulnerable to free radical damage and becomes an easy target organ.…”

Section: Introductionmentioning

confidence: 99%

p38 and ERK1/2-Dependent Activation of c-Jun Is Required for the Downregulation of Oxidative Stress-Induced ERα in Hypothalamic Astrocytes

Dai

Lin

et al. 2023

Neuroendocrinology

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Introduction: Gonadotropin-releasing hormone (GnRH) is a hypothalamic neuropeptide that plays important roles in the female fertility. Accumulating evidence suggests that ERα present in the astrocytes of the hypothalamus region is essential for production of GnRH. The astrocytes display age-related senescence associated to oxidative stress induced by the estrogen metabolites. However, it is still unclear whether and how ERα expression changes during astrocyte aging. Methods: Immunofluorescence was performed to analyze the ERα gene levels in hypothalamic astrocytes of natural aging C57BL/6J female mice. We employed an oxidative stress cell model receiving 2OH-E2 intervention to confirm the downregulation of ERα expression in primary astrocytes. Western blot analysis was used to explore which oxidative stress signaling pathways induced loss of the ERα gene. Finally, ChIP-qPCR was employed to evaluate whether the c-Jun protein is able to regulate ERα gene expression. Results: Compared to young mice, we found that the ERα expression of mid-aged mice was significantly decreased. In hypothalamic astrocytes, 2OH-E2 treatment significantly reduced the expression of the ERα gene. Moreover, we observed that transcription factor c‐Jun could directly inhibit transcriptional ERα gene expression and might also reduce it by decreasing H3K27 acetylation at promotor regions. Administration of the antioxidants Rg1 and AST significantly attenuated the decrease in ERα gene expression induced by oxidative stress. Conclusions: The current data demonstrate that oxidative stress leads to loss of ERα involving the activation of the p38 and ERK1/2 pathways and the induction of the c-Jun protein in hypothalamic astrocytes. C-Jun protein regulates ERα gene expression via direct transcriptional repression or involving histone acetylation modifications at ERα gene promoter sites.

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The remarkable impact of Opuntia Ficus Indica fruit administration on metabolic syndrome: Correlations between cognitive functions, oxidative stress and lipid dysmetabolism in the high-fat, diet-fed rat model

Di Majo,

Ricciardi,

Di Liberto

et al. 2024

Biomedicine & Pharmacotherapy

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Dietary interventions in mice affect oxidative stress and gene expression of the Prlr and Esr1 in the adipose tissue and hypothalamus of dams and their offspring

Cited by 7 publications

References 39 publications

High Prolactin Concentration Induces Ovarian Granulosa Cell Oxidative Stress, Leading to Apoptosis Mediated by L-PRLR and S-PRLR

High Prolactin Concentration Induces Ovarian Granulosa Cell Oxidative Stress, Leading to Apoptosis Mediated by L-PRLR and S-PRLR

p38 and ERK1/2-Dependent Activation of c-Jun Is Required for the Downregulation of Oxidative Stress-Induced ERα in Hypothalamic Astrocytes

The remarkable impact of Opuntia Ficus Indica fruit administration on metabolic syndrome: Correlations between cognitive functions, oxidative stress and lipid dysmetabolism in the high-fat, diet-fed rat model

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