Dietary Interventions of Salmon and Silver Carp Phospholipids on Mice with Metabolic Syndrome Based on Lipidomics

Abstract

The number of metabolic syndromes (MetS) is increasing, and a fish phospholipid diet can reduce the risk of MetS. In this study, the changes in lipid metabolism of colon contents were analyzed by extensive lipidomics in mice with metabolic syndrome by fish phospholipid diet, and mice were randomly divided into experimental groups with different diet types by establishing a MetS model. After 14 weeks, the mice were sacrificed and the serum and colon contents were collected. Ultra-high liquid phase tandem mass spectrometry was used for broadly targeted lipidomic analysis, and the qualitative and quantitative detection of lipid metabolism changes in the colonic contents of mice. Under the intervention of fish phospholipids, MetS mice were significantly inhibited, serum total cholesterol (TC) and triglycerides (TG) decreased, serum high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) levels were improved, fasting blood glucose and insulin levels decreased, and inflammatory factors decreased. Through screening, it was found that thirty-three lipid metabolites may be key metabolites and five have significantly changed metabolic pathways. Modularizing lipid metabolites, it is possible to understand the extent to which different types and concentrations of fish phospholipids affect metabolic syndrome. Therefore, our study may provide new therapeutic clues for improving MetS.

Has-miR-199a-3p/RELA/SCD inhibits immune checkpoints in AMD and promotes macrophage-mediated inflammation and pathological angiogenesis through lipid metabolism pathway: A computational analysis

“…TCGA data were screened for differentially expressed genes using the ANOVA method (| log2FC | cutoff>1 and P<0.01 were considered significantly different). All relevant analyses were conducted using the Spearman method, and statistical plotting was performed using the ggplot2 and pheatmap packages of R software [ 30 ].…”

β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model

“…Based on the results of OPLS-DA, the variable importance in projection (VIP) values of OPLS-DA multivariate analysis were used to preliminarily screen out lipids with different varieties or tissues [43]. Meanwhile, the p-value or fold change (FC) obtained from univariate analysis was integrated to effectively identify DELMs.…”

“…Meanwhile, the p-value or fold change (FC) obtained from univariate analysis was integrated to effectively identify DELMs. FC ≥ 1, VIP ≥ 1, and p-value < 0.05 were set as the screening criteria to screen DELMs [43]. A combination of POS and NEG ion modes yielded 3305 DELMs in the B group compared with the HFD group, of which 1764 metabolites were upregulated and 1541 were downregulated (Figure 6A).…”