Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp ( Ctenopharyngodon idella )

Deng, Yu-Ping; Jiang, Wei‐Dan; Liu, Yang; Qu, Biao; Jiang, Jun; Kuang, Sheng‐Yao; Tang, Ling; Tang, Wu‐Neng; Wu, Pei; Zhang, Yongan; Zhou, Xiao‐Qiu; Feng, Lin

doi:10.1016/j.aquaculture.2015.11.007

Cited by 44 publications

(16 citation statements)

References 63 publications

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“…Several researches found the expressions of NQO1, GCLC and GSTA were up-regulated in a Nrf2-dependent manner (Solis et al, 2002;Nioi et al, 2003). At 24 h, Nrf2, NQO1 and GCLC gene expressions were repressed at lower concentrations, similar observation was also showed in Deng's report (Deng et al, 2016). We speculated the uptake of DCF during initial stage of exposure was not enough to trigger the accumulation of reactive oxygen species (ROS) in fish and subsequently induced transcriptional expression of antioxidant-related genes.…”

Section: Discussion 41 Sequences Analysis Of Nrf2 Nqo1 Gclc Sod2supporting

confidence: 76%

“…As known to all, GSH is a reductive "pool" to GSTs, thus GSTs activity may be restrained ultimately with GSH content (Boyer., 1989;Mukanganyama et al, 2011). Similar reports were found in many researches in which it was explained that the detoxification system may cope with the insults of xenobiotics via the existing reductive pool at low concentrations, but with rising concentrations, the accumulation of ROS ultimately blocked the responses of antioxidant system at the higher concentration (Ma et al, 2012;Deng et al, 2016).…”

Section: Enzyme Response Ofsupporting

confidence: 65%

“…There were many reports of genes expression performance as U-shape, displaying the induction at lower concentrations and inhibition at higher concentrations (Ma et al, 2012;Brox et al, 2016). This phenomenon might be attributed to the activation of the detoxification system at lower concentrations exposure, but under higher concentrations excess and accumulation of ROS (H 2 O 2 ) that blocked the transcriptional expressions, exhibiting a trend of inhibition (Solis et al, 2002;Nioi et al, 2003;Deng et al, 2016). GST, SOD and CAT, the change of GSH and MDA content in G. affinis to DCF exposure Oxidative stress occurs when the balance between ROS and antioxidant system is disrupted.…”

Section: Transcriptional Changes Ofmentioning

confidence: 99%

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Effects of diclofenac on the expression of Nrf2 and its downstream target genes in mosquito fish (Gambusia affinis)

Bao

Nie

et al. 2017

Aquatic Toxicology

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Diclofenac (DCF) is one of widely used non-steroidal anti-inflammatory drugs. Recently, this drug has been universally detected in aquatic environment. However, its potential adverse effects and oxidative stress toxic mechanisms on fish remain unclear. In the present study, we first cloned the crucial partial sequences of some key oxidative stress related genes, which include NF-E2-related factor 2 (Nrf2), NAD(P)H: quinoneoxidoreductase (NQO1), glutamate-cysteine ligase catalytic subunit (GCLC), Cu-Zn superoxide dismutase (SOD2), catalase (CAT), alpha-glutathione S-transferase (GSTA), and UDP-glucuronosyltransferases (UGT) in mosquito fish (Gambusia affinis). We also deduced amino acids of Nrf2 and then constructed the phylogenetic trees of Nrf2, NQO1 and GCLC, respectively. Results showed that a high identity percentage was founded between G. affinis and other bony fish species, such as Xiphophorus maculates and Poecilia reticulate. The transcriptional expression of these genes and partly related enzymes activities were then investigated under the included environmental relevant concentration DCF exposure (0μmolL, 1.572×10μmolL, 1.572×10μmolL, 0.1572μmolL and 1.572μmolL) for 24h and 168h. The expression of Nrf2 was inhibited at 24h but induced at 168h, exhibiting a significant time and/or dose-effect relationship under DCF exposure. Similar observation was found in its downstream target genes. However, Nrf2-mediated antioxidant enzymes activities displayed differently under the same concentration of DCF exposure for the same time. Under DCF exposure for 168h, the genes exhibited dramatic induction trend, but there were no significant changes in enzyme activities and MDA content. Overall, mRNA responses were more sensitive than enzyme changes in mosquito fish under DCF exposure.

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Section: Discussion 41 Sequences Analysis Of Nrf2 Nqo1 Gclc Sod2supporting

confidence: 76%

Section: Enzyme Response Ofsupporting

confidence: 65%

Section: Transcriptional Changes Ofmentioning

confidence: 99%

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Effects of diclofenac on the expression of Nrf2 and its downstream target genes in mosquito fish (Gambusia affinis)

Bao

Nie

et al. 2017

Aquatic Toxicology

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“…Blood samples of five fish from each tank were drawn from the caudal vein using heparinized syringes for PAC determination ( Wang et al., 2015 ). Then another five fish from each tank were anesthetized in 50 mg/L benzocaine bath ( Deng et al., 2016 ) and sampled for determination of protein content, and trypsin, chymotrypsin, lipase and amylase activities in hepatopancreas and intestine, CK, Na + , K + -ATPase, AKP, and γ-GT activities in intestine, glutamic oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) activities in hepatopancreas and muscle ( Feng et al., 2013 , Tang et al., 2013 ).…”

Section: Methodsmentioning

confidence: 99%

Growth rate, body composition, digestive enzymes and transaminase activities, and plasma ammonia concentration of different weight Jian carp (Cyprinus carpio var. Jian)

et al. 2015

Self Cite

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The present study investigated the effect of body weight on body composition, digestive and absorptive capacity, transaminase activities in hepatopancreas and muscle, and plasma ammonia concentration of Jian carp (Cyprinus carpio var. Jian). A total of 750 Jian carps (18.0 ± 0.2 g) were randomly distributed into five groups with three replicates and fed the same diet for 56 days. Tissue and plasma samples were collected on days 14, 28, 42, and 56. The results were used to develop a mathematical model for specific growth rate, body moisture and fat content, aspartate transaminase activity and alanine aminotransferase activity in hepatopancreas and muscle, plasma ammonia concentration, and trypsin, chymotrypsin, lipase, and amylase activities in hepatopancreas and intestine, activities of creatine kinase, Na+/K+-ATPase, alkaline phosphatase, and γ-glutamyl transpeptidase in intestine in Jian carp. There were linear relationships between natural logarithms of above indexes and body weight. The body moisture and fat content, digestive and absorptive enzymes activities, and transaminase activities showed negative allometry against body weight of Jian carp which were partial reasons to explain fish growth rate decreasing.

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“…For instance, Bai et al indicated that low BCAA levels inhibited fatty acid synthesis and enhanced FAO in the liver of the female broiler chickens [143]. Moreover, Deng et al demonstrated that optimum Leu levels improved flesh quality, partly by regulating antioxidant-related signaling molecules leading to an enhanced antioxidant capacity [144]. In growing pigs, Duan et al showed that a low-protein diet with the appropriate ratios of BCAAs was able to increase growth, and as a result regulate lipid metabolism in the skeletal muscle [34].…”

Section: Application In Livestock Productionmentioning

confidence: 99%

Leucine Supplementation: A Novel Strategy for Modulating Lipid Metabolism and Energy Homeostasis

Zhang

Guo

et al. 2020

Nutrients

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Lipid metabolism is an important and complex biochemical process involved in the storage of energy and maintenance of normal biological functions. Leucine, a branched amino acid, has anti-obesity effects on glucose tolerance, lipid metabolism, and insulin sensitivity. Leucine also modulates mitochondrial dysfunction, representing a new strategy to target aging, neurodegenerative disease, obesity, diabetes, and cardiovascular disease. Although various studies have been carried out, much uncertainty still exists and further studies are required to fully elucidate the relationship between leucine and lipid metabolism. This review offers an up-to-date report on leucine, as key roles in both lipid metabolism and energy homeostasis in vivo and in vitro by acceleration of fatty acid oxidation, lipolysis, activation of the adenosine 5′-monophosphate-activated protein kinase (AMPK)–silent information regulator of transcription 1 (SIRT1)–proliferator-activated receptor γ coactivator-1α (PGC-1α) pathway, synthesis, and/or secretion of adipokines and stability of the gut microbiota.

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Dietary leucine improves flesh quality and alters mRNA expressions of Nrf2-mediated antioxidant enzymes in the muscle of grass carp ( Ctenopharyngodon idella )

Cited by 44 publications

References 63 publications

Effects of diclofenac on the expression of Nrf2 and its downstream target genes in mosquito fish (Gambusia affinis)

Effects of diclofenac on the expression of Nrf2 and its downstream target genes in mosquito fish (Gambusia affinis)

Growth rate, body composition, digestive enzymes and transaminase activities, and plasma ammonia concentration of different weight Jian carp (Cyprinus carpio var. Jian)

Leucine Supplementation: A Novel Strategy for Modulating Lipid Metabolism and Energy Homeostasis

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