Dietary leucine modulates growth performance, Nrf2 antioxidant signaling pathway and immune response of juvenile blunt snout bream ( Megalobrama amblycephala )

Liang, Hualiang; Mokrani, Amina; Ji, Ke; Ge, Xianping; Ren, Mingchun; Xie, Jun; Liu, Bo; Xi, Bingwen; Zhou, Qunlan

doi:10.1016/j.fsi.2017.11.048

Cited by 52 publications

(30 citation statements)

References 55 publications

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“…Leucine, a member of the branched-chain amino acid family, is a nutritionally essential amino acid in animals (Li, Yin, Tan, Kong, & Wu, 2011). Growing evidences showed that optimal leucine level can improve the antioxidant capacity in rats (Li et al, 2013) and in fish (Deng et al, 2014;Giri et al, 2015;Liang et al, 2018). However, very limited information has as yet been reported about the effects of leucine on antioxidant status in the pigs.…”

Section: Discussionmentioning

confidence: 99%

“…In fish, expression of Nrf2 gene was elevated and Keap1 was decreased by optimum dietary leucine supplementation (Deng et al, 2014;Liang et al, 2018). In fish, expression of Nrf2 gene was elevated and Keap1 was decreased by optimum dietary leucine supplementation (Deng et al, 2014;Liang et al, 2018).…”

Section: Mammalian Cells Have a Complex Network Of Antioxidant Enzymesmentioning

confidence: 99%

“…Branched chain amino acids (BCAAs) are three (leucine, valine, and isoleucine) of the eleven essential amino acids in pigs and have been implicated in many cellular metabolic processes such as protein metabolism and energy production (Duan et al, 2016;Wu, 2014). In addition, leucine has been demonstrated to improve antioxidant capacity by Nrf2 signaling pathway in fish (Deng et al, 2014;Giri et al, 2015;Liang et al, 2018). In addition, leucine has been demonstrated to improve antioxidant capacity by Nrf2 signaling pathway in fish (Deng et al, 2014;Giri et al, 2015;Liang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Effects of dietary leucine on antioxidant activity and expression of antioxidant and mitochondrial‐related genes in longissimus dorsi muscle and liver of piglets

Chen

Xiang

Jia

et al. 2019

Animal Science Journal

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The study was conducted to investigate the effects of dietary leucine on antioxidant activity and expression of antioxidant-and mitochondrial-related genes in longissimus dorsi muscle and liver of piglets. Three diets were formulated with different levels of supplemented leucine (0%, 0.25%, 0.5%). Results showed that supplementation of 0.25% leucine significantly increased antisuperoxide anion (ASA) and antihydroxyl radical (AHR) levels and activities of total superoxide dismutade (T-SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), and total antioxidant capacity (T-AOC) in serum, longissimus dorsi muscle and liver of piglets as compared with the control group. The SOD2, catalase (CAT), GPx, GST, glutathione reductase (GR), and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA levels in longissimus dorsi muscle and liver were significantly increased by 0.25% leucine supplementation. However, the malondialdehyde (MDA) content and the mRNA level of Kelchlike ECH-associated protein 1 (Keap1) exhibited an opposite tendency. Additionally, supplementation of 0.25% leucine significantly increased the mRNA levels of mitochondrial-related genes in longissimus dorsi muscle and liver of piglets. Results suggested that supplementation of 0.25% leucine improved antioxidant activity and mitochondrial biogenesis and function of piglets, which was related to the increase in antioxidant enzymes activities and upregulation of expression of antioxidant-and mitochondrial-related genes. K E Y W O R D S antioxidant activity, leucine, mitochondrial, piglets | 991 CHEN Et al.

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Section: Discussionmentioning

confidence: 99%

Section: Mammalian Cells Have a Complex Network Of Antioxidant Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of dietary leucine on antioxidant activity and expression of antioxidant and mitochondrial‐related genes in longissimus dorsi muscle and liver of piglets

Chen

Xiang

Jia

et al. 2019

Animal Science Journal

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“…Plasma biochemical indices are known to be as an indicator of the general status of fish health (Kavitha, Ramesh, Kumaran, & Lakshmi, ; Liang, Ji, et al, ; Liang, Mokrani, et al, ). In addition, plasma urea usually used as a reference for the dysfunction of the fish kidney (Bernet, Schmidt, Wahli, & Burkhardt‐Holm, ).…”

Section: Discussionmentioning

confidence: 99%

“…The annual production of the latter species reached approximately 820,000 tons in 2016 (Yearbook, ). This species is economically important in China because of its high survival rate, fast growth, high disease resistance, natural food and excellent flesh (Liang, Ji, et al, ; Liang, Mokrani, et al, ; Zhou, Ren, Zeng, & Yao, ). It has been introduced in many countries worldwide such as in Africa, North America and Eurasia (Habte‐Tsion et al, ).…”

Section: Introductionmentioning

confidence: 99%

Complete replacement of fish meal by plant protein ingredients with dietary essential amino acids supplementation for juvenile blunt snout bream ( Megalobrama amblycephala )

et al. 2018

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Six extruded diets were formulated with a graded level of fish meal (FM); diet 1 and diet 2 were formulated with 80 g/kg FM; diet 3 and diet 4 were formulated with 40 g/kg FM; and diet 5 and diet 6 were devoid of FM. Hence, diet 2, diet 4 and diet 6were supplemented with essential amino acids (EAAs). The diets were fed throughout an eight-week feeding trial. The results revealed that specific growth rate (SGR), feed conversion ratio (FCR), body weight gain (BWG) and protein efficiency ratio (PER) were influenced by FM reduction and improved by the addition of EAA (p < 0.05). A survival rate (SR), whole body content of protein, lipid, moisture and ash, and plasma total protein (TP), albumin (ALB), triglyceride (TG) and urea (UN) did not influence by dietary treatments (p > 0.05). Additionally, target of rapamycin (TOR) pathway did not influence by dietary treatments (p > 0.05). Expression levels of hepatic peptide transporter 1 (Pept1) and peptide transporter 2 (Pept2) decreased against FM reduction and improved significantly in the groups fed diet 4 and diet 6.In summary, the findings revealed that diet containing plant proteins mixture supplemented with EAA could totally replace FM in the practical diet of blunt snout bream. K E Y W O R D Sblunt snout bream (Megalobrama amblycephala), essential amino acids supplementation, extruded feed, fish meal reduction

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Leucine protects bovine intestinal epithelial cells from hydrogen peroxide‐induced apoptosis by alleviating oxidative damage

Mao

Zhang

et al. 2022

J Sci Food Agric

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BACKGROUND The present study aimed to investigate whether leucine (Leu) alleviates oxidative injury in bovine intestinal epithelial cells (BIECs) induced by hydrogen peroxide (H2O2), as well as the underlying molecular mechanisms. RESULTS BIECs were treated with H2O2 (1 mmol L−1) and/or Leu (0, 0.9, 1.8 or 3.6 mmol L−1) for 2 h. Leu increased cell viability (P < 0.05) and decreased the release of lactate dehydrogenase (P < 0.05) in BIECs challenged by H2O2. Then, the cells were treated with H2O2 (1 mmol L−1) and/or Leu (1.8 mmol L−1) for 2 h. Compared with the H2O2 group, cells treated with Leu and Leu + H2O2 exhibited increased (P < 0.05) mRNA and protein expression of superoxide dismutase 2 (SOD2), catalase (CAT), glutathione peroxidase 1 (GPx1), heme oxygenase 1 (HO‐1) and nuclear factor erythroid 2‐related factor 2 (Nrf2). BIECs treatment with Leu significantly reduced (P < 0.05) apoptosis induced by H2O2. BIECs were transfected with Nrf2 small interfering RNA (siRNA) for 48 h and/or treated with H2O2 (1 mmol L−1) and/or Leu (1.8 mmol L−1) for another 2 h. Transfection with Nrf2 siRNA abrogated the protective effect of Leu against H2O2‐induced apoptosis and the mRNA and protein expression of SOD2 (P < 0.05). CONCLUSION These results indicate that Leu promotes the relative expression of antioxidant enzymes (SOD2, CAT and GPx1) and phase II detoxification enzymes (HO‐1) by upregulating nuclear Nrf2 and activating the Nrf2 signaling pathway, thus enhancing the antioxidant capacity of cells. © 2022 Society of Chemical Industry.

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Dietary leucine modulates growth performance, Nrf2 antioxidant signaling pathway and immune response of juvenile blunt snout bream ( Megalobrama amblycephala )

Cited by 52 publications

References 55 publications

Effects of dietary leucine on antioxidant activity and expression of antioxidant and mitochondrial‐related genes in longissimus dorsi muscle and liver of piglets

Effects of dietary leucine on antioxidant activity and expression of antioxidant and mitochondrial‐related genes in longissimus dorsi muscle and liver of piglets

Complete replacement of fish meal by plant protein ingredients with dietary essential amino acids supplementation for juvenile blunt snout bream ( Megalobrama amblycephala )

Leucine protects bovine intestinal epithelial cells from hydrogen peroxide‐induced apoptosis by alleviating oxidative damage

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