Dietary methionine sources and levels modulate the intestinal health status of broiler chickens

Gong, Lu; Mahmood, Tahir; Mercier, Yves; Xu, Huiping; Zhang, Xiaodan; Zhao, Yizhu; Luo, Yimeng; Guo, Yuming

doi:10.1016/j.aninu.2023.07.004

Cited by 8 publications

(10 citation statements)

References 62 publications

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“…Our previous results showed that there were no differences in growth performance between two Met sources, which is also supported by other studies. ,, The growth performance of broilers in this study did not reach the breeder recommendations, and we speculate that the long period of 20 d without adjustment of nutritional settings is likely a major reason. Interestingly, our results showed that low TSAA/Lys ratio groups had better BW and FCR than middle and high TSAA/Lys ratio ones at 13 days and these were not significantly different from the recommended TSAA/Lys ratio even at 20 days . It has been confirmed in the native breed of broiler chicks (Zhuanghe Dagu) that dietary Met restriction (Met level was 80% of the control group) had no negative effects on body weight gain .…”

Section: Discussionsupporting

confidence: 62%

“…1,2 Further studies have shown that OH-Met treatment elevated the intestinal antioxidant capacity in piglets, 3 prevented the increase in paracellular permeability induced by H 2 O 2 or TNF-α in Caco-2 cells, 4 and prompted positive effects on the intestinal health status of broilers. 5 It is of relevance to understand the differences in transport and metabolism of different Met sources to rationally supply Met. Furthermore, the appropriate digesta pH plays an integral role in the intestinal health of chickens.…”

Section: Introductionmentioning

confidence: 99%

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Methionine Source and Level Modulate Gut pH, Amino Acid Transporters and Metabolism Related Genes in Broiler Chickens

Gong,

Xu,

Zhang

et al. 2024

J. Agric. Food Chem.

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This study determined the effects of two methionine (Met) sources at three total sulfur amino acids (TSAA) to lysine ratios (TSAA/Lys) on gut pH, digestive enzyme activity, amino acid transporter expression, and Met metabolism of broilers. The birds were randomly assigned to a 2 × 3 factorial arrangement with Met sources (DL-Met and DL-2-hydroxy-4-(methylthio)-butanoic acid (OH-Met)) and TSAA/Lys (0.58, 0.73, and 0.88) from 1 to 21 days. The results demonstrated that DL-Met and OH-Met supported the same growth performance, but high TSAA/Lys ratio reduced the feed intake and body weight (P < 0.05). OH-Met reduced the crop chyme pH and enhanced the jejunal lipase activity (P < 0.05). ATB 0,+ expression decreased with increased DL-Met levels in the duodenum; the low TSAA/Lys ratio induced a stronger mRNA expression of basolateral Met transporters. OH-Met resulted in an increase of cystathionine β-synthase expression in the liver and a decrease in serum homocysteine levels at middle TSAA/Lys ratio compared with DL-Met treatment (P < 0.05). In conclusion, two Met sources support the same growth, but OH-Met acidified the crop chyme. The investigated transporter transcripts differed significantly along the small intestine. At the middle TSAA/Lys ratio, OH-Met showed a higher metabolic tendency of the trans-sulfuration pathway compared with DL-Met.

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Section: Discussionsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Methionine Source and Level Modulate Gut pH, Amino Acid Transporters and Metabolism Related Genes in Broiler Chickens

Gong,

Xu,

Zhang

et al. 2024

J. Agric. Food Chem.

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“…However, it also led to a signi cant increase in fat pad rate, while breast rate remained lower than that of the Normalprotein Control group. Methionine's role in poultry has been extensively studied [31][32] . Apart from its involvement in protein synthesis, methionine can also enhance overall body metabolism.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the dietary amino acids profiles and the relative biomarkers for amino acids balance in the low-protein diets for broiler chickens

Wang,

Zhang,

Liu

et al. 2024

Preprint

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Background: The researches on low-protein-level diets (LPD) indicated that even the profiles of essential amino acids (EAA) follow the recommendation for the normal-protein-level diets (NPD), broilers fed low-protein diets failed to achieve comparable productive performance as those fed with normal diets. Therefore, it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance. Furthermore, finding novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acids nutrition and application technology. In this study, twelve dietary treatments (Con(+), Con(-), L&A(-), L&A(+), M&C(-), M&C(+), BCAA(-), BCAA(+), Thr(-), Thr(+), Trp(-) and Trp(+)) were established by combining different EAAs including Lysine and Arginine, methionine and Cysteine, branched-chain amino acid (BCAA), threonine and tryptophan in order to observe the growth and development of the broiler chickens fed with low-protein-level diets. Based on biochemical parameters and metabolomics analysis from animals subjected to different treatments, were conducted to identify biomarkers associated with optimal and suboptimal amino acid balances. Results: The growth performance, carcass characteristic, hepatic enzyme activities, serum biochemical parameters, and breast muscle mRNA expression showed significant differences between male and female broilers under different dietary amino acid patterns. Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns compared to female broilers. For the low-protein diet, it was indicated that the dietary concentrations of lysine, arginine, and tryptophan were needed to be increased while not for the methionine, cystine, and threonine concentrations. Further research is needed on each individual BCAA. For metabolomic analysis, Con(+) was selected as a normal diet (NP) while Con(-) represented a low-protein diet (LP). L&A(+) denoted a low-protein amino acid balanced diet (LPAB) and Thr(+) represented a low-protein amino acid imbalance diet (LPAI). Metabolites oxypurinol, pantothenic acid, and D-octopine in the birds were significantly influenced by the different dietary amino acid paterns. Conclusion: Adjusting the profile of amino acids in low-protein diets is required to obtain normal growth performance of broiler chickens as fed with normal-protein-level diets. Oxypurinol, pantothenic acid, and D-octopine were identified as potential sensitive biomarkers for assessing amino acid balance.

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“…On the genus level, the proportion of beneficial bacteria such as Lactobacillus in the ileum of the LPS group was lower than that in the saline group, while the proportion of Escherichia-Shigella was higher. Lactobacillus is a common probiotic in the phylum Bacteroidota ( 91 ), which can ferment in the intestine to produce lactic acid, reduce intestinal pH to inhibit the growth of harmful bacteria, and effectively maintain the acid–base balance of the intestine ( 92 ). It can also help to enhance immune function and provide nutritional support to promote intestinal health ( 93 ).…”

Section: Discussionmentioning

confidence: 99%

Aspirin eugenol ester affects ileal barrier function, inflammatory response and microbiota in broilers under lipopolysaccharide-induced immune stress conditions

Zhang,

Bai,

Zhen

et al. 2024

Front. Vet. Sci.

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AimsThe aim of this study was to investigate the effects of aspirin eugenol ester (AEE) on ileal immune function in broilers under lipopolysaccharide (LPS)-induced immune stress.MethodsTwo hundred and forty one-day-old male Arbor Acres chicks were randomly divided into four groups (saline, LPS, saline + AEE and LPS + AEE) with six replicates of ten broilers each. The saline group and LPS group were fed the normal diet, while the other two groups received normal diet plus 0.1 g/kg AEE. Broilers in the LPS and LPS + AEE groups were injected intraperitoneally with 0.5 mg/kg B.W LPS in saline for seven consecutive days beginning at 14 days of age, while broilers in the saline and saline + AEE groups were injected with saline only.ResultsThe results showed that AEE improved the ileal morphology and increased the ratio of villus height to crypt depth of immune-stressed broilers. LPS-induced immune stress significantly reduced the expression of the genes for the tight junction proteins occludin, zonula occludens-1 (ZO-1), claudin-1 and claudin-2, in the ileum, while AEE significantly up-regulated the expression of these genes. Compared with the saline group, the LPS-treated chickens showed significantly increased mRNA expression of the inflammatory factors tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-10 (IL-10), cyclooxygenase-2 (COX-2), and microsomal Prostaglandin E Synthesase-1 (mPGES-1) in the ileum, while they were significantly decreased by AEE supplementation. In addition, analysis of the ileal bacterial composition showed that compared with saline and LPS + AEE groups, the proportion of Firmicutes and Lactobacillus in the LPS group was lower, while the proportion of Proteobacteria and Escherichia-Shigella was higher. Similarly, Line Discriminant Analysis Effect Size (LEfSe) analysis showed that compared with the LPS group, Brevibacillus was dominant in the saline group, while the LPS + AEE group was rich in Rhizobium, Lachnoclostridium, Ruminococcaceae, Faecalibacterium, Negativibacillus, Oscillospiraceae, and Flavonifractor.ConclusionThese results indicate that dietary supplementation with 0.1 g/kg AEE could protect the intestinal health by improving the intestinal villus morphology, enhancing the expression of tight junction genes and alleviating inflammation to resist the immune stress caused by LPS stimulation in broilers, and the mechanism may involve COX-2-related signal transduction and improved intestinal microbiota composition.

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Dietary methionine sources and levels modulate the intestinal health status of broiler chickens

Cited by 8 publications

References 62 publications

Methionine Source and Level Modulate Gut pH, Amino Acid Transporters and Metabolism Related Genes in Broiler Chickens

Methionine Source and Level Modulate Gut pH, Amino Acid Transporters and Metabolism Related Genes in Broiler Chickens

Assessment of the dietary amino acids profiles and the relative biomarkers for amino acids balance in the low-protein diets for broiler chickens

Aspirin eugenol ester affects ileal barrier function, inflammatory response and microbiota in broilers under lipopolysaccharide-induced immune stress conditions

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