Dietary modulation of small intestinal glutamine transport in intestinal brush border membrane vesicles of rats

Salloum, Rabih M.; Souba, Wiley W.; Fernandez, Alarico; Stevens, Bruce R.

doi:10.1016/0022-4804(90)90244-v

Cited by 47 publications

(25 citation statements)

References 10 publications

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“…Gln absortion by System L may also be inhibited by L-alanine, L-serine and L-cysteine (Pan et al, 2004). In rat, high doses of dietary Gln supplementation (30% total dietary nitrogen provided by Gln) were shown to increment up to 75% the absorption capacity of the small intestine BBMV (Salloum et al, 1990). Our results, however, did not reveal any effect of dietary Gln supplementation on Gln, Arg or Glu vesicular uptake.…”

Section: Organmentioning

confidence: 62%

Dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles

Coutinho

Castro

Rufino‐Palomares

et al. 2016

Comparative Biochemistry and Physiology Part A: Molecular &

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Section: Organmentioning

confidence: 62%

Dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles

Coutinho

Castro

Rufino‐Palomares

et al. 2016

Comparative Biochemistry and Physiology Part A: Molecular &

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“…Secondly, the linear increase in crypt depth as glutamine intake increased is indicative of an increase in glutamine metabolism in the mucosa. An increase in glutamine transport across the enterocytes increases the activity of mitochondrial glutaminase (Klimberg et al, 1990); Salloum et al, 1990) that would, in turn, accelerate the hydrolysis of glutamine into products that can directly enter the tricarboxylic acid cycle and generate adenosine triphosphate. This energy could be used to support cell division in the proliferative zone in the crypts of Lieberkiihn (Newsholme, Crabtree and Ardawi, 1985;Klimberg et al, 1990) since glutamine is a requisite substrate for de novo DNA biosynthesis.…”

Section: Structure and Function Of The Small Intestine In Piglets Offmentioning

confidence: 99%

“…Glutamine is the principal fuel used by the gut epithelium (Windmueller, 1982), is present in high concentrations in sow's milk around the time of weaning (Wu and Knabe, 1994), and numerous studies have demonstrated that glutamine is required by villous enterocytes to support metabolism, and the structure and function of the gut (Souba, 1991(Souba, , 1992(Souba, and 1993. Provision of oral glutamine stimulates net uptake of glutamine by enhancing brush-border transport rates (Salloum, Souba, Fernandez and Stevens, 1990), and supports mucosal growth by stimulating the activity of glutaminase (Klimberg, Salloum, Kasper, Plumley, Dolson, Hautamaki, Mendenhall, Bova, Bland, Copeland and Souba, 1990;Salloum, Souba, Klimberg, Plumley, Dolson, Bland and Copeland, 1989). At a time when the piglet's supply of maternal glutamine disappears, supplementation of diets with synthetic glutamine offers a means of enhancing the structure and function of the gut after weaning.…”

Section: Introductionmentioning

confidence: 99%

Maintenance of villous height and crypt depth in piglets by providing continuous nutrition after weaning

1996

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(3) and (4) (P < 0-05 and P = 0-073, respectively). In turn, villous height was significantly correlated (r = 0-78 to 0-87, P < 0-05) with the rate of bodyweight gain after weaning in these two groups. For piglets offered ewes' milk plus glutamine, an increase in DM intake was associated only with increases in crypt depth (P < 0-01). These data show that the structure and function of the small intestine can be preserved when a milk diet is given after weaning, and suggest an association between food intake and villous height in determining post-weaning weight gain. were offered ewes' fresh milk every 2 h in a feeding schedule that increased from 1-2 I per piglet on the 1st day after weaning to 2-4 I on days 4 and 5. On the 5th day all piglets were killed and samples of small intestine were taken for histological and biochemical examination. Feeding ewes' milk or ewes' milk plus 20 g L-glutamine per I maintained (P > 0-05) villous height and crypt depth compared with piglets killed at weaning. In contrast, piglets given a dry starter diet had shorter villi (P < 0-001), deeper crypts (P < 0-001), and proportionately 0-21 to 0-28 less protein (P>0-05) in their intestinal mucosa. Piglets given the starter diet proportionately grew from 0-49 to 0-62 more slowly (P < 0-01), ate the same amount of dry matter (DM; P > 0-05), but consumed proportionately 0-30 less energy (P < 0-001) than their counterparts given the milk diets. No treatment differences in the specific activity of lactase and sucrase were observed (P>0-05). Significant correlations existed between voluntary food intake and villous height at the proximal jejunum for piglets given the starter diet and ewes' milk

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“…The Na + -dependent mechanism has been described as electrogenic, sensitive to alanine, serine, cysteine, histidine and asparagine but insensitive to MeAIB and BCH and does not accept choline, Li + or K + [14,16,25,30,31,39]. Although it has been proposed that this mechanism is system B 0 , system B 0 accepts BCH [23].…”

Section: Glutamine Transport In Small Intestinal Villous and Crypt Cellsmentioning

confidence: 99%

“…In small intestinal brush border membrane vesicles [14,31] and cultured human intestinal cancer Caco-2 cells [34] l-glutamine transport has been ascribed to systems B 0 and L. Additionally, it has been shown that lglutamine is transported by systems B 0 , ASC and b 0,+ [24,25]. System A has also been proposed as a carrier for lglutamine in isolated intestinal cells [5] and intestinal basolateral plasma membrane vesicles [40].…”

Section: Introductionmentioning

confidence: 99%

Glutamine transport in isolated epithelial intestinal cells. Identification of a Na + -dependent transport mechanism, highly specific for glutamine

Castillo

Sulbaran-Carrasco

Burguillos

2002

Pfl�gers Archiv European Journal of Physiology

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L-glutamine transport was evaluated in isolated cells from the guinea-pig small intestine by measuring [(3)H]- L-glutamine uptake. Villous and crypt cells expressed Na(+)-dependent and Na(+)-independent transport mechanisms. Glutamine transport systems were identified using various amino acids and analogues as inhibitors. In both villous and crypt cells, 2-(methylamino)-isobutyrate (MeAIB), a system A inhibitor, did not inhibit Na(+)-dependent glutamine influx. 2-Aminobicyclo(2,2,1)heptane-2-carboxylate (BCH), a system B(0) and B(0,+) substrate, had no effect on Na(+)-dependent influx. Serine, cysteine and threonine, system ASC inhibitors, reduced Na(+)-dependent influx by 50%. Asparagine, but not histidine, system N inhibitors, reduced Na(+)-dependent glutamine influx by 50%, however the effect of asparagine was not additive to that of threonine. The remaining Na(+)-dependent glutamine influx (50%) was only inhibited by glutamine itself, by Na(+) substitution ( N-methyl-glucamine, K(+), Li(+)) or by external pH reduction. Phenyl-acetyl-glutamine (PAG), a synthetic amino acid analogue, also inhibited this Na(+)-dependent, threonine-insensitive glutamine influx (IC(50) 2.45 mM). The Na(+)-independent uptake was partially inhibited by BCH, a system L inhibitor, and other neutral amino acids, but was not affect by PAG. Our results suggest that glutamine is transported in both villous and crypt cells by the Na(+)-independent system L, by the Na(+)-dependent system ASC and by an as yet undescribed Na(+)-dependent transport mechanism, highly specific for glutamine.

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Dietary modulation of small intestinal glutamine transport in intestinal brush border membrane vesicles of rats

Cited by 47 publications

References 10 publications

Dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles

Dietary glutamine supplementation effects on amino acid metabolism, intestinal nutrient absorption capacity and antioxidant response of gilthead sea bream (Sparus aurata) juveniles

Maintenance of villous height and crypt depth in piglets by providing continuous nutrition after weaning

Glutamine transport in isolated epithelial intestinal cells. Identification of a Na + -dependent transport mechanism, highly specific for glutamine

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