Dietary nucleotide-rich yeast supplementation improves growth, innate immunity and intestinal morphology of Pacific white shrimp (Litopenaeus vannamei)

Abstract

An 8‐week feeding trial was conducted to evaluate the effects of dietary nucleotide (NT)‐rich yeast supplementation on growth, innate immunity and intestinal morphology in Pacific white shrimp (Litopenaeus vannamei). Four isonitrogenous and isolipidic practical diets were formulated to contain 0 (control), 10, 30 and 50 g/kg of NT‐rich yeast, respectively. A total of 480 shrimp with an average initial body weight of 1.86 ± 0.02 g were randomly allocated into four groups, with four replicates per group and 30 shrimp each replicate. The results indicated that shrimp fed the diet containing 50 g/kg NT‐rich yeast had significantly higher weight gain (WG), specific growth rate (SGR) and protein efficiency ratio (PER) than those fed the control diet, and the lowest feed conversion ratio (FCR) was observed in the shrimp fed the 50 g/kg NT‐rich yeast supplemental diet. However, there was no significant difference in survival among all treatments. The crude protein of whole shrimp in the 50 g/kg NT‐rich yeast group was higher than that in the control group. Total protein, triglyceride concentrations, the activities of aspartate aminotransferase and alanine aminotransferase in serum were significantly influenced by the dietary NT‐rich yeast supplementation. The activities of serum phenoloxidase (PO) and lysozyme (LZM) of shrimp fed the diet containing 50 g/kg NT‐rich yeast were higher than those in shrimp fed the other diets. Relative expressions of alp and lzm significantly upregulated in the 30 g/kg NT‐rich yeast group compared to the control group. The intestinal fold height and fold width in the 30 g/kg NT‐rich yeast group were significantly higher than those fed the control diet; and the highest microvillus height occurred in the shrimp fed the 50 g/kg NT‐rich yeast diet. In summary, dietary 30–50 g/kg NT‐rich yeast supplementation promotes growth performance, enhances innate immunity and improves intestinal morphology of Litopenaeus vannamei.

Functional palatability enhancer improved growth, intestinal morphology, and hepatopancreas protease activity, replacing squid paste in white shrimp, Litopenaeus vannamei, diets

“…Furthermore, supplementation of the diet containing 1% squid visceral paste with the palatability enhancer gave similar results as 3% squid visceral paste. This increase in intestinal fold height will likely result in an enhanced area for nutrient absorption and has been reported previously in white shrimp associated with the dietary supplementation with nucleotides and yeast extracts (Guo et al, 2016;Xiong et al, 2018).…”

The combined effect of vitamin E, arachidonic acid,Haemtococcus pluvialis, nucleotides and yeast extract on growth and ovarian development of crayfish (Cherax quadricarinatus) by the orthogonal array design

“…For instance, Xiong et al. (2018) reported that the dietary supplement of 10 and 30 g/kg YE presented similar final body weight (FBW), specific growth rate (SGR) and FCR with the shrimp control diet. Li et al.…”

Dietary guanosine‐monophosphate improves growth performance, feed utilization and intestinal morphology of whiteleg shrimp ( Litopenaeus vannamei ) maintained on soybean meal‐based diets

“…Therefore, better growth performance and feed utilization in shrimp fed D + G could be linked to the larger absorption area in the intestine. Although there is limited information on the effects of either individual or combinations of nucleotides on shrimp intestinal conditions, there is a great deal of similarity of this study with the other investigations on whiteleg shrimp where commercial nucleotide products such as nucleotide‐rich yeast (Xiong et al., 2018) and commercial nucleotides Rovimax (Guo et al., 2016). Xiong et al.…”

“…Xiong et al. (2018) were reported to have significantly improved the gut condition with greater sizes of intestinal folds contributing to the gut functions. Guo et al.…”