Dietary Oxalate Intake and Kidney Outcomes

Bargagli, Matteo; Tio, Maria Clarissa; Waikar, Sushrut S.; Ferraro, Pietro Manuel

doi:10.3390/nu12092673

Cited by 48 publications

(35 citation statements)

References 130 publications

(175 reference statements)

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“…Spinach belongs to the Chenopodiaceae family in the order Caryophyllales. A number of quantitative trait loci (QTLs), markers and genes associated with agronomic traits such as leaf morphology 2 , 3 , bolting 4 , flowering, and nutritional quality 5 , 6 have been identified for spinach during the past decade; however, there is still an urgent need for spinach improvement, for example, to reduce the concentration of oxalate that can cause health issues when excessively consumed 7 , and to enhance the resistance to major diseases.…”

Section: Introductionmentioning

confidence: 99%

Genomic analyses provide insights into spinach domestication and the genetic basis of agronomic traits

Cai

Sun

et al. 2021

Nat Commun

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Spinach is a nutritious leafy vegetable belonging to the family Chenopodiaceae. Here we report a high-quality chromosome-scale reference genome assembly of spinach and genome resequencing of 305 cultivated and wild spinach accessions. Reconstruction of ancestral Chenopodiaceae karyotype indicates substantial genome rearrangements in spinach after its divergence from ancestral Chenopodiaceae, coinciding with high repeat content in the spinach genome. Population genomic analyses provide insights into spinach genetic diversity and population differentiation. Genome-wide association studies of 20 agronomical traits identify numerous significantly associated regions and candidate genes for these traits. Domestication sweeps in the spinach genome are identified, some of which are associated with important traits (e.g., leaf phenotype, bolting and flowering), demonstrating the role of artificial selection in shaping spinach phenotypic evolution. This study provides not only insights into the spinach evolution and domestication but also valuable resources for facilitating spinach breeding.

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Section: Introductionmentioning

confidence: 99%

Genomic analyses provide insights into spinach domestication and the genetic basis of agronomic traits

Cai

Sun

et al. 2021

Nat Commun

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“…Despite these multiple roles in plants, high oxalate levels in edible plant parts are a concern for human nutrition and health. Excess levels of oxalate in a regular diet significantly alter nutrient availability, as oxalate reacts with calcium and other minerals to form insoluble crystals, resulting in hyperoxaluria, a predominant risk factor for recurrent kidney stones [ 5 , 16 , 17 ]. Hence, in order to better understand its biological relevance in oxalogenic plants and manipulate its accumulation in edible plant parts, elucidating oxalate’s metabolic or gene regulatory mechanisms is essential.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Oxalate Metabolism in Spinach Revealed by RNA-Seq-Based Transcriptomic Analysis

Joshi

Penalosa

Joshi³

et al. 2021

IJMS

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Although spinach (Spinacia oleracea L.) is considered to be one of the most nutrient-rich leafy vegetables, it is also a potent accumulator of anti-nutritional oxalate. Reducing oxalate content would increase the nutritional value of spinach by enhancing the dietary bioavailability of calcium and other minerals. This study aimed to investigate the proposed hypothesis that a complex network of genes associated with intrinsic metabolic and physiological processes regulates oxalate homeostasis in spinach. Transcriptomic (RNA-Seq) analysis of the leaf and root tissues of two spinach genotypes with contrasting oxalate phenotypes was performed under normal physiological conditions. A total of 2308 leaf- and 1686 root-specific differentially expressed genes (DEGs) were identified in the high-oxalate spinach genotype. Gene Ontology (GO) analysis of DEGs identified molecular functions associated with various enzymatic activities, while KEGG pathway analysis revealed enrichment of the metabolic and secondary metabolite pathways. The expression profiles of genes associated with distinct physiological processes suggested that the glyoxylate cycle, ascorbate degradation, and photorespiratory pathway may collectively regulate oxalate in spinach. The data support the idea that isocitrate lyase (ICL), ascorbate catabolism-related genes, and acyl-activating enzyme 3 (AAE3) all play roles in oxalate homeostasis in spinach. The findings from this study provide the foundation for novel insights into oxalate metabolism in spinach.

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“…We sequenced genomes of 305 wild and cultivated spinach accessions, including 295 of S. oleracea collected worldwide, and all available accessions of S. tetrandra (n=3) and S. turkestanica (7) in the U.S. National Plant Germplasm System (Fig. 3a and Supplementary Table 10).…”

Section: Genome Variation Map Of Spinachmentioning

confidence: 99%

“…Spinach belongs to the Chenopodiaceae family in the order Caryophyllales, the basal order of core eudicots. A number of quantitative trait loci (QTLs), markers and genes associated with agronomic traits such as leaf morphology 2,3 , bolting 4 , flowering and nutritional quality 5,6 have been identified for spinach during the past decade; however, there is still an urgent need for spinach improvement, for example, to reduce the concentration of oxalate that can cause health issues when excessively consumed 7 , and to enhance the resistance to major diseases.…”

Section: Introductionmentioning

confidence: 99%

Reference genome and resequencing of 305 accessions provide insights into spinach evolution, domestication and genetic basis of agronomic traits

Cai

Sun

et al. 2021

Preprint

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Spinach is a nutritious leafy vegetable belonging to the family Chenopodiaceae. Here we report a chromosome-scale reference genome assembly of spinach, which has a total size of 894.3 Mb and an N50 contig size of 23.8 Mb, with 98.3% anchored and ordered on the six chromosomes. Reconstruction of ancestral Chenopodiaceae karyotype indicates substantial genome rearrangements in spinach after its divergence from ancestral Chenopodiaceae, coinciding with high repeat content in the spinach genome. Resequencing the genomes of 305 cultivated and wild spinach accessions provides insights into spinach genetic diversity and population differentiation. Genome-wide association studies of 20 agronomical traits identify numerous significantly associated regions and candidate genes for these traits. Domestication sweeps in the spinach genome are identified, some of which are associated with important traits (e.g., leaf phenotype, bolting and flowering), demonstrating the role of artificial selection in shaping spinach phenotypic evolution. This study provides not only insights into the spinach evolution and domestication but also valuable resources for facilitating spinach breeding.

show abstract

Dietary Oxalate Intake and Kidney Outcomes

Cited by 48 publications

References 130 publications

Genomic analyses provide insights into spinach domestication and the genetic basis of agronomic traits

Genomic analyses provide insights into spinach domestication and the genetic basis of agronomic traits

Regulation of Oxalate Metabolism in Spinach Revealed by RNA-Seq-Based Transcriptomic Analysis

Reference genome and resequencing of 305 accessions provide insights into spinach evolution, domestication and genetic basis of agronomic traits

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