Dietary Pectin with High Viscosity Lowers Plasma and Liver Cholesterol Concentration and Plasma Cholesteryl Ester Transfer Protein Activity in Hamsters

Abstract

We fed semipurified diets containing pectin with either a high or low in vitro viscosity at a level of 3 g/100 g air-dried diet to hamsters for 8 wk. A control group was fed cellulose and a positive control group was fed psyllium. The pectins used were a calcium-sensitive pectin (CS-pectin) that has a high viscosity and a noncalcium-sensitive pectin (NCS-pectin) that has a low viscosity. In the presence of calcium, CS-pectin has a more than 80-fold higher viscosity than NCS-pectin which offered the opportunity to investigate the possible role of viscosity in the hypolipidemic properties of pectin. The hamsters fed CS-pectin or psyllium had considerably lower plasma cholesterol concentrations (3.69 +/- 0.44 and 4.21 +/- 0.45 mmol/L, respectively, mean +/- SD, n = 14) than those fed NCS-pectin (5.03 +/- 1.15 mmol/L) or cellulose (5.72 +/- 1. 04 mmol/L). Differences in total plasma cholesterol were reflected in both high density lipoprotein and very low density lipoprotein cholesterol. There was no effect of fiber on low density lipoprotein cholesterol levels. Liver cholesterol concentrations paralleled the plasma cholesterol levels and were 9.91 +/- 2.48 micromol/g of liver for the CS-pectin group, 15.03 +/- 5.75 for the psyllium group, 17. 69 +/- 10.66 for the NCS-pectin group, and 25.57 +/- 9.23 for the cellulose group. Fecal bile acid and neutral steroid excretion tended to be higher in the hamsters fed CS-pectin than in their counterparts fed NCS-pectin. The hamsters fed psyllium had significantly greater fecal excretions of bile acids than the hamsters fed cellulose, CS-pectin or NCS-pectin, whereas the excretion of fecal neutral sterols tended to be lower. Plasma cholesteryl ester transfer protein activity was significantly lower in the hamsters fed CS-pectin than in those fed NCS-pectin. The results of this study suggest that the viscosity of pectins may determine their cholesterolemic effect.

Anti-fat deposition and antioxidant effects of haw pectic oligosaccharide in the liver of high-fat-fed mice

“…As a soluble food fiber, pectin is known to decrease the levels of total cholesterol (TC) and TG in the blood (Lattimer & Haub, 2010;Terpstra, Lapre, Vries, & Beynen, 1998). We previously demonstrated that HPOS had a greater capacity to lower serum TG and TC (Li, Wang, Du, & Guo, 2008;Li et al, 2010).…”

Pectin of Prunus domestica L. alters sulfated structure of cell-surface heparan sulfate in differentiated Caco-2 cells through stimulation of heparan sulfate 6-O-endosulfatase-2

“…Passage 70-85 Caco-2 cells were cultured at 37°C with 5% CO 2 in Dulbecco's Modified Eagle's Medium (Nissui Pharmaceuticals, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS), 1% minimal essential medium non-essential amino acids, 80 units/ mL of penicillin, 80 μg/mL of streptomycin, 0.06% of L-glutamine, and 0.19% of NaHCO 3 .…”

“…Pectin reduces plasma and liver cholesterol levels, 3) prevents hepatic metastasis, 4) inhibits allergic responses, 5) exhibits anticancer activities, 2,6) and decreases the risks of intestinal infection and diarrhea in children. 7) Heparan sulfate (HS) is a sulfated polysaccharide that is ubiquitously present on the cell surface and in the extracellular matrix of all animal cells.…”

Efeito do consumo de tremoço (Lupinus albus) e seu isolado protéico no metabolismo do colesterol em hamsters hipercolesterolemizados

“…Após a adição de solução saturada de NaCl, o colesterol foi extraído três vezes com 3 mL de éter de petróleo (TERPSTRA et al, 1998). A amostra foi seca em estufa a 40-45°C com circulação de ar e ressuspensa em 800 µL de hexano grau CLAE.…”