Dietary Phenethyl Isothiocyanate Protects Mice from Colitis Associated Colon Cancer

Liu, Yi; Dey, Moul

doi:10.3390/ijms18091908

Cited by 20 publications

(6 citation statements)

References 40 publications

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“…Induction of mitochondria caspase cascade and JNK, critical factors for the initiation of apoptotic was observed in PEITC treated HT-29, colon cancer cell [ 82 ]. Anti-inflammatory effect of PEITC against colon cancer was correlated with the lesser expression of NF-κB [ 83 ].…”

Section: Molecular Mechanism Of Itcs In Cancer Preventionmentioning

confidence: 99%

“…These results suggest that the interference of β-catenin binding on the TBE0 site by p65 is involved in the suppression of cyclin D1 gene expression [ 134 ]. BITC significantly enhanced the nuclear translocation and increased the sensitivity of NF-κB [ 83 ]. Further, Liu and Dey (2010) reported that PEITC inhibits NF-κB in a concentration-dependent manner.…”

Section: Direct and Indirect Inhibition Of Nf-κb By Itcsmentioning

confidence: 99%

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Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers

2018

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Glucosinolates (GSL) are naturally occurring β-d-thioglucosides found across the cruciferous vegetables. Core structure formation and side-chain modifications lead to the synthesis of more than 200 types of GSLs in Brassicaceae. Isothiocyanates (ITCs) are chemoprotectives produced as the hydrolyzed product of GSLs by enzyme myrosinase. Benzyl isothiocyanate (BITC), phenethyl isothiocyanate (PEITC) and sulforaphane ([1-isothioyanato-4-(methyl-sulfinyl) butane], SFN) are potential ITCs with efficient therapeutic properties. Beneficial role of BITC, PEITC and SFN was widely studied against various cancers such as breast, brain, blood, bone, colon, gastric, liver, lung, oral, pancreatic, prostate and so forth. Nuclear factor-erythroid 2-related factor-2 (Nrf2) is a key transcription factor limits the tumor progression. Induction of ARE (antioxidant responsive element) and ROS (reactive oxygen species) mediated pathway by Nrf2 controls the activity of nuclear factor-kappaB (NF-κB). NF-κB has a double edged role in the immune system. NF-κB induced during inflammatory is essential for an acute immune process. Meanwhile, hyper activation of NF-κB transcription factors was witnessed in the tumor cells. Antagonistic activity of BITC, PEITC and SFN against cancer was related with the direct/indirect interaction with Nrf2 and NF-κB protein. All three ITCs able to disrupts Nrf2-Keap1 complex and translocate Nrf2 into the nucleus. BITC have the affinity to inhibit the NF-κB than SFN due to the presence of additional benzyl structure. This review will give the overview on chemo preventive of ITCs against several types of cancer cell lines. We have also discussed the molecular interaction(s) of the antagonistic effect of BITC, PEITC and SFN with Nrf2 and NF-κB to prevent cancer.

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Section: Molecular Mechanism Of Itcs In Cancer Preventionmentioning

confidence: 99%

Section: Direct and Indirect Inhibition Of Nf-κb By Itcsmentioning

confidence: 99%

Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers

2018

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“…Recent epidemiologic studies show that utilization of cruciferous foods can reduce the event of a threat and diminish the frequency of malignant growth [1,2]. For instance, the intake of cruciferous vegetables helps to reduce the risk of ovarian [3] and colon cancer [4,5], lung cancer [6], and prostate cancer [7,8]. Cruciferous vegetables are in the Brassicaceae family that includes broccoli, cabbage, wasabi, mustard cauliflower, horseradish, etc.…”

Section: Introductionmentioning

confidence: 99%

Eradication of Myrosinase-Tethered Cancer Cells by Allyl Isothiocyanate Derived from Enzymatic Hydrolysis of Sinigrin

2022

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Sinigrin is present in significant amounts in cruciferous vegetables. Epidemiological studies suggest that the consumption of such vegetables decreases the risk of cancer, and the effect is attributed mainly to allyl isothiocyanate (AITC), a hydrolysis product of sinigrin catalyzed by myrosinase. Anticancer activity of AITC has been previously investigated for several cancer models, but less attention was paid to delivering AITC on the target site. In this study, the gene sequences of core streptavidin (coreSA) and myrosinase (MYR) were cloned in a pET-30a(+) plasmid and transformed into BL21(DE3) E. coli competent cells. The MYR-coreSA chimeric protein was expressed and purified using immobilized metal affinity chromatography and further characterized by gel electrophoresis, Western blot, and enzyme activity assay. The purified MYR-coreSA chimeric protein was tethered on the outer membrane of biotinylated adenocarcinoma A549 cells and then treated with various concentrations of sinigrin. Our results showed that 20 µM of sinigrin inhibited the growth of A549 cells tethered with myrosinase by ~60% in 48 h. Furthermore, the levels of treated cells undertaken apoptosis were determined by Caspase-3/7 activation and Annexin-V. In summary, sinigrin harnessed like a prodrug catalyzed by myrosinase to the production of AITC, which induced cell apoptosis and arrested the growth of lung cancer cells.

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“…Phenethyl isothiocyanate (PEITC) is a biologically active dietary compound that is derived from cruciferous vegetables, such as watercress, broccoli, cabbage, and cauliflower [ 28 , 29 ]. We and others have reported anti-inflammatory and chemo-preventive characteristics of PEITC [ 29 , 30 , 31 , 32 , 33 , 34 ]. More recently, we showed that PEITC targets CSCs by promoting apoptosis [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Phenethyl Isothiocyanate Exposure Promotes Oxidative Stress and Suppresses Sp1 Transcription Factor in Cancer Stem Cells

Upadhyaya

Liu

Dey

2019

IJMS

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Aldehyde dehydrogenase 1 (ALDH1) is a cytosolic marker of cancer stem cells (CSCs), which are a sub-population within heterogeneous tumor cells. CSCs associate with therapy-resistance, self-renewal, malignancy, tumor-relapse, and reduced patient-survival window. ALDH1-mediated aldehyde scavenging helps CSCs to survive a higher level of oxidative stress than regular cancer cells. Cruciferous vegetable-derived phenethyl isothiocyanate (PEITC) selectively induces reactive oxygen species (ROS), leading to apoptosis of cancer cells, but not healthy cells. However, this pro-oxidant role of PEITC in CSCs is poorly understood and is investigated here. In a HeLa CSCs model (hCSCs), the sphere-culture and tumorsphere assay showed significantly enriched ALDHhi CSCs from HeLa parental cells (p < 0.05). Aldefluor assay and cell proliferation assay revealed that PEITC treatments resulted in a reduced number of ALDHhi hCSCs in a concentration-dependent manner (p < 0.05). In the ROS assay, PEITC promoted oxidative stress in hCSCs (p ≤ 0.001). Using immunoblotting and flow cytometry techniques, we reported that PEITC suppressed the cancer-associated transcription factor (Sp1) and a downstream multidrug resistance protein (P-glycoprotein) (both, p < 0.05). Furthermore, PEITC-treatment of hCSCs, prior to xenotransplantation in mice, lowered the in vivo tumor-initiating potential of hCSCs. In summary, PEITC treatment suppressed the proliferation of ALDH1 expressing cancer stem cells as well as key factors that are involved with drug-resistance, while promoting oxidative stress and apoptosis in hCSCs.

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Dietary Phenethyl Isothiocyanate Protects Mice from Colitis Associated Colon Cancer

Cited by 20 publications

References 40 publications

Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers

Anti-Carcinogenic Glucosinolates in Cruciferous Vegetables and Their Antagonistic Effects on Prevention of Cancers

Eradication of Myrosinase-Tethered Cancer Cells by Allyl Isothiocyanate Derived from Enzymatic Hydrolysis of Sinigrin

Phenethyl Isothiocyanate Exposure Promotes Oxidative Stress and Suppresses Sp1 Transcription Factor in Cancer Stem Cells

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