Dietary plant soot supplementation improves the intestinal health status of farmed American eels (Anguilla rostrata)

Abstract

The current study evaluated the effect of different dietary plant soot supplementation levels on the intestinal health status of American eels (Anguilla rostrata) cultured in concrete tanks. Nine concrete fish tanks were randomly divided into three groups and fed the diets with plant soot (PS) supplementation levels at 0, 3 g/kg, and 5 g/kg, respectively. After a feeding trial for 60 days, the intestinal tissues were sampled to measure health status parameters. The D-lactate level and DAO activity in the serum of the PS5 group were significantly lower than those of the PS0 group (P <0.05). Compared with the PS0 group, the villi length of the intestine in both PS3 group and PS5 group increased significantly (P<0.05). The muscular thickness of the intestine of the PS5 group was significantly higher than the PS0 group (P<0.05). The microvillus density of the intestine of the American eel was increased obviously in the PS5 group. The intestinal microbiota composition of plant soot groups was beneficially regulated with certain probiotics’ higher relative abundances and some pathogenic bacteria’ lower relative abundance. In conclusion, dietary 5 g/kg plant soot supplementation could benefit the intestinal health of farmed American eels.