Dietary Propolis Supplement Improves Semen Characteristics in Egyptian Buffaloes

Nagy, Wael; Ghoneim, Hanan A.; El-Aziz, Ayman Hassan Abd; Alsenosy, Abd elwahab

doi:10.21608/djvs.2020.33528.1018

Cited by 1 publication

(2 citation statements)

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“…In other words, despite the fact that the low concentration of propolis alone did not affect GPx activity, propolis in higher concentration could by itself raise GPx activity, even in the absence of damaging stimuli. Some studies evidence a significant increase in SOD activity after propolis treatment in erythrocytes, testis, and sperm [ 14 , 21 , 26 ]. However, the results obtained here did not show any alterations in this detoxicant enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…Administration of propolis in rats and rabbits shows an improvement in body and reproductive organs’ weight, an increase in sperm production, motility, count, and quality, and a reduction in abnormal and dead sperm numbers [ 19 , 22 , 23 , 24 , 25 ]. In co-administration with OS-inducers, like cadmium, aluminum, and copper, propolis ameliorated testicular histopathological and biochemical alterations, decreased the number of apoptotic cells, increased the levels of antioxidant enzymes, with a simultaneous decrease in malonaldehyde levels, a marker of lipid peroxidation [ 19 , 21 , 26 , 27 ]. In men, propolis administration was demonstrated to reduce the free radical-induced lipid peroxidation, as well as increase superoxide dismutase (SOD) activity [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Propolis Protects GC-1spg Spermatogonial Cells against Tert-Butyl Hydroperoxide-Induced Oxidative Damage

Duarte,

Feijó,

Luís

et al. 2024

IJMS

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Propolis is a natural resin produced by honeybees with plenty of pharmacologic properties, including antioxidant activity. Oxidative stress disrupts germ cell development and sperm function, with demonstrated harmful effects on male reproduction. Several natural antioxidants have been shown to reduce oxidative damage and increase sperm fertility potential; however, little is known about the effects of propolis. This work evaluated the role of propolis in protecting spermatogonial cells from oxidative damage. Propolis’ phytochemical composition and antioxidant potential were determined, and mouse GC-1spg spermatogonial cells were treated with 0.1–500 µg/mL propolis (12–48 h) in the presence or absence of an oxidant stimulus (tert-butyl hydroperoxide, TBHP, 0.005–3.6 µg/mL, 12 h). Cytotoxicity was assessed by MTT assays and proliferation by Ki-67 immunocytochemistry. Apoptosis, reactive oxygen species (ROS), and antioxidant defenses were evaluated colorimetrically. Propolis presented high phenolic and flavonoid content and moderate antioxidant activity, increasing the viability of GC-1spg cells and counteracting TBHP’s effects on viability and proliferation. Additionally, propolis reduced ROS levels in GC-1spg, regardless of the presence of TBHP. Propolis decreased caspase-3 and increased glutathione peroxidase activity in TBHP-treated GC-1spg cells. The present study shows the protective action of propolis against oxidative damage in spermatogonia, opening the possibility of exploiting its benefits to male fertility.

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Section: Discussionmentioning

confidence: 99%