Dietary protein source and protein/carbohydrate ratio affects appetite regulation-related genes expression in gilthead seabream (Sparus aurata)

Basto-Silva, Catarina; Enes, Paula; Oliva-Teles, Aires; Balbuena-Pecino, Sara; Navarro, Isabel; Capilla, Encarnación; Guerreiro, Inês

doi:10.1016/j.aquaculture.2020.736142

Cited by 15 publications

(24 citation statements)

References 64 publications

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“…The present study is a follow-up to our previous study [28]. While the previous study aimed to evaluate the effect of dietary protein sources (FM vs. PF) and P/CH ratio on gilthead seabream appetite regulation and intermediary metabolism, the present study aims to evaluate, for the first time, the effects of these factors (dietary protein source and P/CH ratio) on gilthead seabream gut function and health, by assessing gut histomorphology, gut microbiota composition, digestive enzymes activity, and gut immunological and oxidative stress genes expression.…”

Section: Introductionmentioning

confidence: 72%

“…During the trial, all experimental diets were well-accepted by the fish, and the fish survival rate was 100%. Results of the rearing trial were not the aim of this study and are presented elsewhere [28].…”

Section: Resultsmentioning

confidence: 99%

“…Fish-rearing conditions are described in detail in Basto-Silva et al [28]. Briefly, 180 gilthead seabream (140 ± 0.1 g, initial body weight) were randomly distributed to twelve 300-L water capacity tanks in a temperature-controlled recirculation life-support system.…”

Section: Experimental Conditions and Samplingmentioning

confidence: 99%

“…The total RNA extraction from intestinal samples, the RNA concentration, the purity and integrity evaluation, the cDNA synthesis, and the quantitative real-time PCR (qPCR) were performed as described in Basto-Silva et al [28]. The forward and reverse primers used (Table 1) were searched in the GenBank database [36], and their efficiency curves were evaluated according to the assay conditions.…”

Section: Rna Extraction Cdna Synthesis and Quantitative Real-time Pcr...mentioning

confidence: 99%

“…Recently, we assessed the effects of FM-or PF-based diets with different P/CH ratios (50/10 and 40/20) in gilthead seabream growth, feed utilization, appetite regulation, and intermediary metabolism [28]. Results showed that diets only slightly modified fish appetite and metabolic parameters, although growth was higher in fish fed the FM-P50/CH10 diet than those fed the FM-P40/CH20 diet.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Dietary Plant Feedstuffs and Protein/Carbohydrate Ratio on Gilthead Seabream (Sparus aurata) Gut Health and Functionality

Basto-Silva

García-Meilán

Couto

et al. 2022

Fishes

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This study investigated, for the first time, the integrated effects of dietary protein source and protein/carbohydrate (P/CH) ratio on gilthead seabream gut histomorphology, microbiota composition, digestive enzymes activity, and immunological and oxidative stress-related gene expressions. Four isolipidic diets: two fishmeal-based (FM) and two plant feedstuff (PF)-based diets, with P/CH ratios of 50/10 or 40/20 each (FM-P50/CH10; FM-P40/CH20; PF-P50/CH10; PF-P40/CH20), were tested. PF-based diets lead to more histomorphological alterations than FM-based diets. P/CH ratio had no relevant effect on gut histomorphology. Gut mucosa of fish fed PF-based diets presented a higher number of operational taxonomic units, and richness and diversity indices, while the P/CH ratio did not affect those parameters. The α-amylase activity was lower in fish fed with PF-based diets and in fish fed the P40/CH20 diets. Regarding the immune-related genes, only cyclooxygenase-2 was affected, being higher in fish fed the P50/CH10 diets than the P40/CH20 diets. Fish fed the FM-based diets presented higher expression of glutathione reductase and glutathione peroxidase, while fish fed the P50/CH10 diet had higher expression of superoxide dismutase. In conclusion, PF-based diets can compromise gut absorptive and digestive metabolism, but decreasing the dietary P/CH ratio had little effect on the parameters measured.

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Section: Introductionmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Section: Experimental Conditions and Samplingmentioning

confidence: 99%

Section: Rna Extraction Cdna Synthesis and Quantitative Real-time Pcr...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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