Dietary Pulverized Konjac Glucomannan Prevents the Development of Allergic Rhinitis-Like Symptoms and IgE Response in Mice

Onishi, Nobukazu; Kawamoto, Seiji; Ueda, Kazuyuki; Yamanaka, Yasushi; Katayama, Akiko; Suzuki, Hidenori; Aki, Tsunehiro; Hashimoto, Kunihiko; Hide, Michihiro; Ono, Kazuhisa

doi:10.1271/bbb.70378

Cited by 40 publications

(24 citation statements)

References 19 publications

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“…Plasma OVA-specific IgE, IgG1 and IgG2a titers were analyzed by ELISA, as previously described [19,20] with some modifications. Briefly, 96-well microtiter plates were coated with OVA solution (100 µg/ml) at 4°C overnight.…”

Section: Methodsmentioning

confidence: 99%

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Suzuki¹,

Oomizu²,

Yanase³

et al. 2009

Int Arch Allergy Immunol

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Background: Oral administration of pulverized Konjac glucomannan (KGM) reduces increased plasma IgE and the amount of Ε-germline transcript (ΕGT) in the spleen, as well as preventing the development of dermatitis in mice. To elucidate the mechanism of action of pulverized KGM, we solubilized KGM and studied its effect on IgE in vitro and in vivo. Methods: Solubilized KGM was prepared by acid hydrolysis, and we analyzed the effective molecular size for the suppression of IgE production and ΕGT in vitro and the level of plasma IgE induced by immunization with ovalbumin in BALB/c mice. Results: The production of IgE and ΕGT in splenic cells, but not purified B cells, was inhibited by hydrolyzed KGM (KGM hydrolyzed with 0.25 N HCl; H-KGM) at the optimal size of between 10 and 500 kDa. However, no effect was observed when H-KGM was substituted with unhydrolyzed KGM in vitro. IgE production from purified B cells cocultured with purified monocytes, but not with purified T cells, was inhibited by H-KGM. The release of IFNγ in cultures of monocytes but in purified B cells with or without T cells was enhanced in the presence of H-KGM. Injection of mice with H-KGM also suppressed the production of plasma IgE and IgG1 but not IgG2a in vivo. Conclusion: KGM at an optimal size prevents germline class-switching and IgE production both in vitro and in vivo. H-KGM may be useful as a tool to study the mechanism of action of KGM and as a dietary supplement to prevent atopic diseases.

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Section: Methodsmentioning

confidence: 99%

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Suzuki¹,

Oomizu²,

Yanase³

et al. 2009

Int Arch Allergy Immunol

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“…To investigate the immunomodulating potency of low-viscous KGM, several particle sizes of pulverized KGM (PKGM) were used in NC/Nga mice, a murine model for human AD [10]. Production of hyper-IgE and inflammatory cytokines was suppressed in the PKGM-fed mice, and AD-like skin symptoms were markedly suppressed by oral intake of PKGM [11,12]. There are several studies on the effects of carbohydrates like inulin, galactomannan, and glucomannan in the form of KGM [13,14].…”

Section: Introductionmentioning

confidence: 99%

Pulverized konjac glucomannan ameliorates oxazolone-induced colitis in mice

et al. 2014

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“…Mannan has been applied widely in the medical field, particularly for the prevention of influenza and bacterial infections, inflammation and allergic diseases [27][28][29] . Based on the previous results and those from the present study, we advocate the use of mannan in the clinical practice of dentistry, as employing a mannan coating on dental devices might improve the oral hygiene.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of coated mannan against the adhesion of Candida biofilms to denture base resin

et al. 2013

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The adherence of Candida on dentures is related to diseases such as denture stomatitis and aspiration pneumonia. Mannan is a major component of the Candida cell surface, and contributes to the cell adherence. A previous report indicated that the adherence of C. albicans to culture dishes was inhibited by the coating them with mannan. The purpose of this study was to examine the adhesion inhibitory effect of mannan coating on acrylic denture surfaces against C. albicans and C. glabrata. The amount of Candida attached on the acrylic surfaces coated with mannan was calibrated by culture methods. Mannan showed significant inhibitory effects on Candida adhesion in both the yeast and hyphal form in a concentration-dependent manner, and the durability of the inhibitory effect continued for three days. These results suggest that mannan coating on the denture base acrylic can prevent Candida adhesion on the denture.

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Dietary Pulverized Konjac Glucomannan Prevents the Development of Allergic Rhinitis-Like Symptoms and IgE Response in Mice

Cited by 40 publications

References 19 publications

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Hydrolyzed Konjac Glucomannan Suppresses IgE Production in Mice B Cells

Pulverized konjac glucomannan ameliorates oxazolone-induced colitis in mice

Inhibitory effect of coated mannan against the adhesion of Candida biofilms to denture base resin

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