Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1

Abstract

BackgroundResveratrol, a plant phenol, affords protection against inflammation and oxidative stress. The objective of this study was to investigate the effects of dietary resveratrol supplementation during pregnancy and lactation on the antioxidant status of sows and piglets and on antioxidant gene expression and pathway in placenta.MethodsForty sows were allotted to 2 dietary treatments 20 d after breeding. Sows were fed a control diet and a control diet with 300 mg/kg resveratrol. Oxidative stress biomarkers and antioxidant enzymes were measured in the placenta, milk, and plasma of sows and piglets. Antioxidant gene expression and protein expression of Kelch-like ECH-associated protein 1-Nuclear factor E2-related factor 2 (Keap1-Nrf2), nuclear factor kappa B-p65 (NFκB-p65) and sirtuin1 (Sirt1) were quantified in the placenta.ResultsDietary resveratrol increased the litter and piglets weaning weights. Antioxidant status in the milk, placenta and plasma of sows and piglets was partially improved by dietary resveratrol. In placenta, Nrf2 protein expression was increased and Keap1 protein expression was decreased by dietary resveratrol. The mRNA expression of antioxidant genes including catalase (CAT), glutathione peroxidase 1 (GPX1), GPX4, superoxide dismutase 1 (SOD1) and heme oxygenase 1 (HO1), and phase 2 detoxification genes, including glutamate-cysteine ligase modifier (GCLM), microsomal glutathione S-transferase 1(MGST1) and UDP glucuronosyltransferase family 1 member A1 (UGT1A1), was increased by dietary resveratrol. Dietary resveratrol also increased Sirt1 and phosphorylated NFκB-p65 protein expression in the placenta. We failed to observe any influences of dietary resveratrol on pro-inflammatory cytokine levels, including those of interleukin 1β (IL-1β), IL-6, IL-8 and tumor necrosis factor α (TNF-α). However, we observed that the mRNA expression of IL-8 in placenta was reduced by maternal resveratrol. In addition, dietary resveratrol showed interactive effects with day of lactation on activities of SOD and CAT and levels of malonaldehyde (MDA) and hydrogen peroxide (H2O2) in milk.ConclusionsDietary resveratrol supplementation during pregnancy and lactation improves the antioxidant status of sows and piglets, which is beneficial to the reproductive performance of sows. Dietary resveratrol regulates placental antioxidant gene expression by the Keap1-Nrf2 pathway and Sirt1 in placenta.

Health Benefits and Molecular Mechanisms of Resveratrol: A Narrative Review

“…Reducing the ischemia-reperfusion injury-induced oxidative stress by inhibiting the activation of p38 MAPK pathway to increase antioxidants like GSH and scavenge free radicals [28] In vivo Rats 5, 10 mg/kg Activating SIRT1 to scavenge ROS [29] In vivo Mice 15, 30, 60 mg/kg Activating AMPK, SIRT1, and Nrf2 associated antioxidant defense pathways to improve systemic oxidative and nitrosative stress [30] In vivo Sows 300 mg/kg Regulating antioxidant gene expression via Keap1/Nrf2 pathway and SIRT1 [31] In vitro HUVECs 10 µM Inducing autophagy via the activation of TFEB [32] In vitro HEK293 cells or HEK293T 5 µg/mL Inducing autophagy via the AMPK-mediated inhibition of mTOR signaling [33] Anti-inflammatory activities…”

Effects of dietary resveratrol supplementation during gestation and lactation of sows on milk composition of sows and fat metabolism of sucking piglets

“…Resveratrol could improve the immune system of piglets (Fu et al, 2018). Dietary resveratrol increased the litter and piglet weaned weight and improved the antioxidant status in the milk, placenta and plasma of sows and piglets (Meng et al, 2018). In addition, resveratrol has been proposed as a potential compound that regulates pig fat metabolism (Zhang, Luo, Yu, Chen, & Chen, 2015).…”

Regulation of functional amino acids on oxidative stress in pigs

“…相关因子2(nuclear factor erythroid 2-related factor 2, NRF2) [11,13] 和丝裂原活化蛋白激酶(mitogen-activated protein kinase, MAPK) [12] 等信号通路. 同时, 自噬、核 kB因子(nuclear factor kappa B, NF-κB)以及肠道微生…”