Dietary selenium levels modulates antioxidant, cytokine and immune response and selenoproteins mRNA expression in rats under heat stress condition

Aderao, Ganesh N.; Jadhav, S.E.; Pattanaik, A.K.; Gupta, Sanjay Kumar; Ramakrishnan, Saravanan; Lokesha, E.; Chaudhary, Pramod; Vasvani, Shalini; Singh, Asmita; Panigrahi, Manjit; Dutta, Narayan; Singh, Gyanendra

doi:10.1016/j.jtemb.2022.127105

Cited by 9 publications

(7 citation statements)

References 38 publications

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“…HS also increases body metabolism, leading to increased nutrient requirements, which are attributed to HS-induced increased excretion of nutrients [55]. Recently, the murine study by Aderao et al, 2023 found that under prolonged HS conditions, the dietary Se requirement may be increased to 460 ppb for improving the antioxidant status and humoral immune response, and cytokine levels, modulating the thyroid and insulin hormone, and the selenoproteins mRNA expression of rats [54]. These results could support the hypothesis that polymorphisms in the HIKESHI gene could affect its capacity to counteract HS stress damage functions in the human body, influencing dietary Se requirements under HS conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The Gene Ontology Analysis indicated the involvement of HIKESHI in cellular response to stress (GO:0033554) and cellular response to heat (GO:0034605). HIKESHI is a nuclear import carrier for HSP70 that operates during heat shock stress [ 54 ]. HIKESHI mediates the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus, attenuating and reversing heat shock-induced nuclear phenotypes [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Admixture and Association Study of Serum Selenium Deficiency to Identify Genetic Variants Indirectly Linked to Selenium Regulation in Brazilian Adults

Moriguchi Watanabe,

Sousa,

Couto

et al. 2024

Nutrients

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Blood selenium (Se) concentrations differ substantially by population and could be influenced by genetic variants, increasing Se deficiency-related diseases. We conducted a genome-wide association study (GWAS) to identify single nucleotide polymorphisms (SNPs) associated with serum Se deficiency in 382 adults with admixed ancestry. Genotyping arrays were combined to yield 90,937 SNPs. R packages were applied to quality control and imputation. We also performed the ancestral proportion analysis. The Search Tool for the Retrieval of Interacting Genes was used to interrogate known protein–protein interaction networks (PPIs). Our ancestral proportion analysis estimated 71% of the genome was from Caucasians, 22% was from Africans, and 8% was from East Asians. We identified the SNP rs1561573 in the TraB domain containing 2B (TRABD2B), rs425664 in MAF bZIP transcription factor (MAF), rs10444656 in spermatogenesis-associated 13 (SPATA13), and rs6592284 in heat shock protein nuclear import factor (HIKESHI) genes. The PPI analysis showed functional associations of Se deficiency, thyroid hormone metabolism, NRF2-ARE and the Wnt pathway, and heat stress. Our findings show evidence of a genetic association between Se deficiency and metabolic pathways indirectly linked to Se regulation, reinforcing the complex relationship between Se intake and the endogenous factors affecting the Se requirements for optimal health.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Admixture and Association Study of Serum Selenium Deficiency to Identify Genetic Variants Indirectly Linked to Selenium Regulation in Brazilian Adults

Moriguchi Watanabe,

Sousa,

Couto

et al. 2024

Nutrients

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“…HS is accepted to have deleterious effects on rabbit health, welfare and potential fertility outcomes and these effects may be aggravated in the era of climate change. Moreover, HS diminishes the immune responses, persuades cellular impairment via augmentation of OS and antioxidants discrepancy (Abdelnour et al, 2021) and increases the synthesis of circulating proinflammatory mediators such as IL-4 and IFNγ (Aderao et al, 2023).…”

Section: Testicular Changesmentioning

confidence: 99%

“…Generally, the fortification of THQ to the buck diets during HS condition improved the liver function, immunity, antioxidant profile, testicular structure, sperm function, semen attributes, and decreased oxidative stress (MDA) and the inflammation process and lastly efficiently utilized for vanquishing the destructive impacts of HS. There were doubts about the fruitful commercialization of natural active compounds as growth promoters or anti-HS mediators that may have potential speculation supplies for animal breeders (Aderao et al, 2023). The existing outcomes also displayed that bucks' diets fortified with THQ significantly improved FBW, daily DBWG and FCR but did not affect FI.…”

Section: Testicular Changesmentioning

confidence: 99%

Effects of Dietary Thymoquinone Inclusion on Antioxidative, Oxidative, Pro-Inflammatory Responses, Semen Attributes and Testicular Changes in Heat-Stressed Rabbit Bucks

AlGaradi,

Sindi,

Al-Gabrif

et al. 2024

Annals of Animal Science

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Heat stress (HS) is considered a severe concern for rabbit buck’s health, welfare and reproductive features. Thymoquinone (THQ) is a natural molecule with robust antioxidant, antimicrobial, and anti-inflammatory properties. Thereafter, three months of feeding research was applied to assess the role of THQ-enriched diets in improving the health, semen attributes and testicular changes of stressed rabbit bucks. Forty rabbit bucks were used in this research, and animals were fed diets containing 0 (THQ0; serve as a heat stress group), 100 (THQ100), 200 (THQ200), and 400 (THQ400) mg/kg of THQ for three months continually during the summer season. Outcomes displayed that THQ significantly enhanced FBW (final body weight), DBWG (daily body weight gain), and FI (feed intake) but did not affect FCR (feed conversion ratio). Compared with the THQ0 group, dietary THQ addition significantly decreased creatinine, TB (total bilirubin), TG (triglycerides), and GGT (gamma-glutamyl transferase) levels. Bucks receiving 200 mg of THQ had higher TAC (total antioxidant capacity) and GSH (glutathione) than other groups. At the same time, MDA was significantly shrunk in THQ-treated groups relative to the THQ group. The levels of interferon-gamma (IFN-ᵞ) and interleukin-4 (IL-4) were significantly diminished (P <0.001), but nitric oxide and lysosome activity were significantly increased in bucks given diets containing THQ (200 and 400 mg/kg) as relative to the TH0 group. Libido score and reaction time were significantly improved by THQ dietary inclusion. Semen attributes, including volume, motility, concentration, and vitality, were significantly augmented in THQ100 and THQ200 groups. Histological screening of testicular tissues showed moderate to severe degenerative and necrotic changes in the testicular tissues of bucks exposed to HS. This was enhanced with the supplementation of THQ at different levels. Collectively, THQ (200 or 400mg /kg diet) can improve health, semen attributes and restore testicular damage in rabbit bucks via boosting antioxidant and reducing inflammatory cytokines.

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“…Since humans cannot synthesise SeMet, it meets the criteria of an essential amino acid. Although selenium in high concentrations in plasma is considered quite toxic [ 2 ], appropriate levels are considered important, since Se is involved in major metabolic pathways, antioxidant defence [ 3 ], redox reactions in detoxification and chemo-preventive pathways [ 1 ]. Moreover, Se is a micronutrient with potential pharmaceutical application in aiding treatment or the prevention of specific diseases, including cardiovascular disorders [ 4 , 5 ], thyroid dysfunction [ 6 , 7 ] and neurodegenerative diseases [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Selenomethionine Entrapped in Nanoparticles for Oral Supplementation Using In Vitro, Ex Vivo and In Vivo Models

et al. 2023

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Selenium methionine (SeMet) is an essential micronutrient required for normal body function and is associated with additional health benefits. However, oral administration of SeMet can be challenging due to its purported narrow therapeutic index, low oral bioavailability, and high susceptibility to oxidation. To address these issues, SeMet was entrapped in zein-coated nanoparticles made from chitosan using an ionic gelation formulation. The high stability of both the SeMet and selenomethionine nanoparticles (SeMet-NPs) was established using cultured human intestinal and liver epithelial cells, rat liver homogenates, and rat intestinal homogenates and lumen washes. Minimal cytotoxicity to Caco-2 and HepG2 cells was observed for SeMet and SeMet-NPs. Antioxidant properties of SeMet were revealed using a Reactive Oxygen Species (ROS) assay, based on the observation of a concentration-dependent reduction in the build-up of peroxides, hydroxides and hydroxyl radicals in Caco-2 cells exposed to SeMet (6.25–100 μM). The basal apparent permeability coefficient (Papp) of SeMet across isolated rat jejunal mucosae mounted in Ussing chambers was low, but the Papp was increased when presented in NP. SeMet had minimal effects on the electrogenic ion secretion of rat jejunal and colonic mucosae in Ussing chambers. Intra-jejunal injections of SeMet-NPs to rats yielded increased plasma levels of SeMet after 3 h for the SeMet-NPs compared to free SeMet. Overall, there is potential to further develop SeMet-NPs for oral supplementation due to the increased intestinal permeability, versus free SeMet, and the low potential for toxicity.

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Dietary selenium levels modulates antioxidant, cytokine and immune response and selenoproteins mRNA expression in rats under heat stress condition

Cited by 9 publications

References 38 publications

Genome-Wide Admixture and Association Study of Serum Selenium Deficiency to Identify Genetic Variants Indirectly Linked to Selenium Regulation in Brazilian Adults

Genome-Wide Admixture and Association Study of Serum Selenium Deficiency to Identify Genetic Variants Indirectly Linked to Selenium Regulation in Brazilian Adults

Effects of Dietary Thymoquinone Inclusion on Antioxidative, Oxidative, Pro-Inflammatory Responses, Semen Attributes and Testicular Changes in Heat-Stressed Rabbit Bucks

Evaluation of Selenomethionine Entrapped in Nanoparticles for Oral Supplementation Using In Vitro, Ex Vivo and In Vivo Models

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