Dietary Supplementation of Ginger and Turmeric Rhizomes Modulates Platelets Ectonucleotidase and Adenosine Deaminase Activities in Normotensive and Hypertensive Rats

Abstract

Hypertension is associated with platelet alterations that could contribute to the development of cardiovascular complications. Several studies have reported antiplatelet aggregation properties of ginger (Zingiber officinale) and turmeric (Curcuma longa) with limited scientific basis. Hence, this study assessed the effect of dietary supplementation of these rhizomes on platelet ectonucleotidase and adenosine deaminase (ADA) activities in Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME) induced hypertensive rats. Animals were divided into seven groups (n = 10): normotensive control rats; induced (l-NAME hypertensive) rats; hypertensive rats treated with atenolol (10 mg/kg/day); normotensive and hypertensive rats treated with 4% supplementation of turmeric or ginger, respectively. After 14 days of pre-treatment, the animals were induced with hypertension by oral administration of l-NAME (40 mg/kg/day). The results revealed a significant (p < 0.05) increase in platelet ADA activity and ATP hydrolysis with a concomitant decrease in ADP and AMP hydrolysis of l-NAME hypertensive rats when compared with the control. However, dietary supplementation with turmeric or ginger efficiently prevented these alterations by modulating the hydrolysis of ATP, ADP and AMP with a concomitant decrease in ADA activity. Thus, these activities could suggest some possible mechanism of the rhizomes against hypertension-derived complications associated to platelet hyperactivity. Copyright © 2016 John Wiley & Sons, Ltd.

Effect of p ‐coumaric acid on the erectogenic enzyme activities and non‐protein thiol level in the penile tissue of normal and doxorubicin‐induced oxidative stress male rat

“…The multifunction-intracellular nonenzymatic antioxidant effect of non-protein thiol, which is considered to be the main thiol-disulphide redox buffer of the cells, has been reported to functions as protectant of cells from ROS activity (Akinyemi et al, 2016;Akomolafe, Oboh, Oyeleye, & Boligon, 2016). The diminished non-protein thiol level as noted in the DOX-treated rats ( Figure 3c) could be linked to the extensive utilisation of these antioxidant molecules for the scavenging free radicals.…”

“…The diminished non-protein thiol level as noted in the DOX-treated rats ( Figure 3c) could be linked to the extensive utilisation of these antioxidant molecules for the scavenging free radicals. The ROS produced by DOX deplete the intracellular thiol groups as well as unsettle the intracellular organelles (Akinyemi et al, 2016).…”

Hypercholesterolemia, angiotensin converting enzyme and ecto-enzymes of purinergic system: Ameliorative properties of caffeic and chlorogenic acid in hypercholesterolemic rats

“…Hypercholesterolemia has been implicated as a cause of morbidity and mortality (Wald & Law, ). Alteration in the activity of ecto‐enzymes, such as ecto‐nucleoside triphosphate diphosphohydrolase (E‐NTPDase), 5′ nucleotidase, and adenosine deaminase (ADA) has been implicated and linked to the pathogenesis of cardiovascular disease (Akinyemi et al, ), multiple sclerosis (Spanevello, Mazzanti & Bagatini, ), ischemic heart disease (Bagatini et al, ), and lung cancer (Zanini, Schmatz, & Pimentel, ). Literature has shown that e‐NTPDase and adenosine deaminase activity were modulated in hypercholesterolemic rats (Ruchel et al, ).…”

“…However, there is considerable evidence that ATP acts as a neurotransmitter, co‐transmitter in autonomic, sensory nerves, and as a presynaptic modulator (Burnstock, ). It should be noted that the vasodilatory effect of ATP could be halted by the activity of e‐NTPDase which hydrolyzed ATP to ADP, AMP, and adenosine (Akinyemi et al, ). Findings from this work agreed with Baldisser et al () on modulatory effect of plant bioactive compounds on e‐NTPDase and 5′ nucleotidase activities in hypercholesterolemic rats.…”

Sensory evaluation of gluten-free quinoa whole grain snacks

“…Skin health benefits of oral and topical turmeric supplements and products have been observed (Vaughn et al, 2016). Higher blood pressure inducing drug was observed to be ineffective in rats when diet contained 4% ginger or turmeric (Akinyemi et al, 2016). Both heat loss and heat production were increased by consuming cayenne pepper (Kobayashi et al, 1998).…”