Dietary supplementation of krill oil attenuates inflammation and oxidative stress in experimental ulcerative colitis in rats

Grimstad, Tore; Bjørndal, Bodil; Cacabelos, Daniel; Aasprong, Ole Gunnar; Janssen, Emiel A. M.; Omdal, Roald; Svardal, Asbjørn; Hausken, Trygve; Bohov, Pavol; Portero‐Otín, Manuel; Pamplona, Reinald; Berge, Rolf K.

doi:10.3109/00365521.2011.634025

Cited by 62 publications

(68 citation statements)

References 36 publications

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“…Crypt injury and inflammation severity scores were assessed as described earlier ( 17 ) . Both the crypt and inflammatory score include a measure of involvement: grade 1, 1–25 %; grade 2, 26–50 %; grade 3, 51–75 %; grade 4, 76–100 %.…”

Section: Experimental Methodsmentioning

confidence: 99%

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A salmon peptide diet alleviates experimental colitis as compared with fish oil

et al. 2013

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Fish oil (FO) has been shown to have anti-inflammatory properties in animal models of inflammatory bowel disease, but how fish peptides (FP) influence intestinal inflammation has been less studied. Male Wistar rats, divided into five groups, were included in a 4-week dietary intervention study. Of the groups, four were exposed in the fourth week to 5 % dextran sulfate sodium (DSS) to induce colitis, while one group was unexposed. The diets were: (1) control, (2) control + DSS, (3) FO (5 %) + DSS, (4) FP (3·5 %) + DSS, (5) FO + FP + DSS. Following DSS intake, weight and disease activity index (DAI) were assessed, and histological combined score (HCS), selected colonic PG, cytokines, oxidative damage markers and mRNA levels were measured. FP reduced HCS, tended to lower DAI (P = 0·07) and reduced keratinocyte chemoattractant/growth-regulated oncogene levels, as compared with the FO diet. FP also reduced mRNA levels of Il-6 and Cxcl1, although not significantly. FO intake increased the DAI as compared with DSS alone. PGE3 levels increased after the FO diet, and even more following FO + FP intake. The FP diet seems to have a protective effect in DSS-induced colitis as compared with FO. A number of beneficial, but non-significant, changes also occurred after FP v. DSS. A combined FO + FP diet may influence PG synthesis, as PGE3 levels were higher after the combined diet than after FO alone.

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Section: Experimental Methodsmentioning

confidence: 99%

“…Full wall sections of the colon were used, and 50 mg samples were homogenised and prepared as described previously ( 17 ) .…”

Section: Experimental Methodsmentioning

confidence: 99%

A salmon peptide diet alleviates experimental colitis as compared with fish oil

et al. 2013

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“…Lipids from liver extracts or plasma were then measured enzymatically on a Hitachi 917 system (Roche Diagnostics, Mannheim, Germany), using kits for analyzing total TAG (GPO-PAP kit, Roche Diagnostics), cholesterol (CHOD-PAP kit, Roche Diagnostics), and total phospholipids (Diagnostic Systems GmbH, Holzheim, Germany). Fatty acid composition was analyzed in extracted liver lipid, using gas chromatography, as described previously by Grimstad et al (2012).…”

Section: Lipid and Fatty Acid Analysismentioning

confidence: 99%

Three differently generated salmon protein hydrolysates reveal opposite effects on hepatic lipid metabolism in mice fed a high-fat diet

et al. 2015

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This study investigates the effects of salmon peptide fractions, generated using different enzymatic hydrolyzation methods, on hepatic lipid metabolism. Four groups of mice were fed a high-fat diet with 20% casein (control group) or 15% casein and 5% of peptide fractions (treatment groups E1, E2 and E4) for 6weeks. Weight gain was reduced in mice fed E1 and E4-diets compared to control, despite a similar feed intake. Reduced plasma and liver triacylglycerol levels in E1 and E4-mice were linked to reduced fatty acid synthase (FAS) activity and hepatic expression of lipogenic genes. By contrast, plasma and liver lipids increased in the E2 group, concomitant with increased hepatic FAS activity and Δ9 desaturase gene expression. Shotgun lipidomics showed that MUFAs were significantly reduced in the E1 and E4 groups, whereas PUFAs were increased, and the opposite was observed in the E2 group. In conclusion, bioactive peptides with distinctive properties could potentially be isolated from salmon hydrolysates.

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“…Por otra parte, dado el relevante papel de los radicales libres en la etiología de la CU (6) y que diferentes sustancias con propiedades antioxidantes han resultado efectivas en modelos experimentales de CU (27,31,32) , es lógico suponer que los efectos antioxidantes del D-002 estén involucrados en su eficacia en este modelo, lo cual deberá ser dilucidado por ulteriores estudios.…”

Section: Discussionunclassified

Efectos en ratas de los alcoholes de cera de abejas (D-002) sobre la colitis ulcerativa inducida por sulfato de dextrano y etanol

Molina-Cuevas

Ravelo-Calzado

Zamora-Rodríguez

et al. 2017

Rev Peru Med Exp Salud Publica

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RESUMENObjetivos. Investigar los efectos del D-002, mezcla de seis alcoholes alifáticos primarios de alto peso molecular, obtenida de la cera de abejas (Apis mellifera), sobre la colitis ulcerativa (CU) inflamatoria severa inducida por sulfato de dextrano (DSS) y etanol en ratas (Ratus ratus). Materiales y métodos. Las ratas se distribuyeron aleatoriamente en seis grupos: un control cero al que no se provocó daño, y cinco a los que se les indujo la CU: un control negativo (vehículo), tres tratados con D-002 (25, 100 y 400 mg/kg) y un control positivo con sulfazalacina (200 mg/kg) (sustancia de referencia). Se cuantificaron las manifestaciones clínicas (variación del peso corporal, presencia de diarrea y de sangrado rectal), el puntaje de daño macroscópico e histológico, y la actividad de mieoloperoxidasa (MPO). Resultados. El tratamiento oral con D-002 (25, 100 y 400 mg/kg) previno significativamente la disminución del peso corporal. La dosis de 400 mg/kg redujo la presencia de diarreas y sangrado rectal, aunque su comparación con el control negativo solo alcanzó significación estadística sobre las diarreas. El D-002 (25, 100 y 400 mg/kg) redujo significativamente el puntaje de las lesiones macroscópicas (40,0; 43,3 y 47,2% de inhibición, respectivamente), el puntaje de daño histológico (31,5; 53,7 y 67,1% de inhibición, respectivamente) y la actividad de MPO (73,2; 83,6 y 85,0% de inhibición, respectivamente), comparado con el grupo control negativo. La sulfazalacina redujo significativamente todas las variables estudiadas. Conclusiones. El D-002 (25, 100 y 400 mg/kg) protegió significativamente la mucosa colónica en ratas con CU inflamatoria severa inducida por DSS y etanol. Palabras clave: Abejas; Colitis ulcerosa; Ratas (Fuente: DeCS BIREME). EFFECTS IN RATS OF BEE-WAX ALCOHOLS (D-002) ON ULCERATIVE COLITIS INDUCED BY DEXTRAN SULFATE AND ETHANOL ABSTRACTObjectives. To investigate the effects of D-002, a mixture of 6 high molecular weight primary aliphatic alcohols, obtained from beeswax (Apis mellifera), on severe inflammatory ulcerative colitis (UC) induced by Dextran sulfate (DSS) and ethanol in rats (Ratus ratus). Materials and methods. Rats were randomly distributed in six groups: a zero control to which no damage was caused, and five to which the UC was induced: a negative control (vehicle), three treated with D-002 (25, 100 and 400 mg/kg) and a positive control with sulfasalazine (200 mg/kg) (reference substance). Clinical manifestations (body weight variation, diarrhea and rectal bleeding), macroscopic and histological damage score, and myeloperoxidase (MPO) activity were quantified. Results. The oral treatment with D-002 (25, 100 and 400 mg/ kg) significantly prevented the decrease in body weight. The dose of 400 mg/kg reduced the presence of diarrhea and rectal bleeding, although its comparison with the negative control only reached statistical significance on diarrhea. D-002 (25, 100 and 400 mg/kg) significantly reduced the score of macroscopic lesions (40.0; 43.3 and 47.2% inhibition, respectively...

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Dietary supplementation of krill oil attenuates inflammation and oxidative stress in experimental ulcerative colitis in rats

Cited by 62 publications

References 36 publications

A salmon peptide diet alleviates experimental colitis as compared with fish oil

A salmon peptide diet alleviates experimental colitis as compared with fish oil

Three differently generated salmon protein hydrolysates reveal opposite effects on hepatic lipid metabolism in mice fed a high-fat diet

Efectos en ratas de los alcoholes de cera de abejas (D-002) sobre la colitis ulcerativa inducida por sulfato de dextrano y etanol

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