Dietary supplementation with soybean lecithin increases pulmonary PAF bioactivity in asthmatic rats

Muehlmann, Luís Alexandre; Zanatta, Ana L.; Farias, Carolina Lane Alves; Bieberbach, Eloyse W.R.; Mazzonetto, Ana C.; Michellotto, Pedro V.; Fernandes, Luiz C.; Nishiyama, Anita

doi:10.1016/j.jnutbio.2009.03.001

Cited by 12 publications

(9 citation statements)

References 55 publications

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“…Then, BALF was obtained by exposing the trachea, inserting an 18G intravenous catheter (Solidor, Osasco, SP, Brazil), and slowly infusing 1 ml of sterile saline through the catheter and aspirating three times. The recovered BALF was processed as described by Muehlmann et al 18 . BALF supernatants were stored at −20°C for total protein, hydrogen peroxide (H 2 O 2 ), and interleukin (IL) determination.…”

Section: Methodsmentioning

confidence: 99%

Lung Tissue Damage Associated with Allergic Asthma in BALB/c Mice Could Be Controlled with a Single Injection of Mesenchymal Stem Cells from Human Bone Marrow up to 14 d After Transplantation

et al. 2020

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Mesenchymal stem cell (MSC) research has demonstrated the potential of these cells to modulate lung inflammatory processes and tissue repair; however, the underlying mechanisms and treatment durability remain unknown. Here, we investigated the therapeutic potential of human bone marrow-derived MSCs in the inflammatory process and pulmonary remodeling of asthmatic BALB/c mice up to 14 d after transplantation. Our study used ovalbumin to induce allergic asthma in male BALB/c mice. MSCs were injected intratracheally in the asthma groups. Bronchoalveolar lavage fluid (BALF) was collected, and cytology was performed to measure the total protein, hydrogen peroxide (H2O2), and proinflammatory (IL-5, IL-13, and IL-17A) and anti-inflammatory (IL-10) interleukin (IL) levels. The lungs were removed for the histopathological evaluation. On day zero, the eosinophil and lymphochte percentages, total protein concentrations, and IL-13 and IL-17A levels in the BALF were significantly increased in the asthma group, proving the efficacy of the experimental model of allergic asthma. On day 7, the MSC-treated group exhibited significant reductions in the eosinophil, lymphocyte, total protein, H2O2, IL-5, IL-13, and IL-17A levels in the BALF, while the IL-10 levels were significantly increased. On day 14, the total cell numbers and lymphocyte, total protein, IL-13, and IL-17A levels in the BALF in the MSC-treated group were significantly decreased. A significant decrease in airway remodeling was observed on days 7 and 14 in almost all bronchioles, which showed reduced inflammatory infiltration, collagen deposition, muscle and epithelial thickening, and mucus production. These results demonstrate that treatment with a single injection of MSCs reduces the pathophysiological events occurring in an experimental model of allergic asthma by controlling the inflammatory process up to 14 d after transplantation.

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Section: Methodsmentioning

confidence: 99%

Lung Tissue Damage Associated with Allergic Asthma in BALB/c Mice Could Be Controlled with a Single Injection of Mesenchymal Stem Cells from Human Bone Marrow up to 14 d After Transplantation

et al. 2020

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“…BALF total protein concentrations, BALF nitrite and lipid hydroperoxide concentrations in BALF cells, as well as alveolar macrophage phagocytosis and production of superoxide anion and hydrogen peroxide (H 2 O 2 ) were described in another study 22 . Additionally, alveolar macrophage adhesion assay was performed together with the study of alveolar macrophage function as different amounts of cells adhere to the wells of the acrylic plate in each experiment.…”

Section: Methodsmentioning

confidence: 99%

Platelet-Activating Factor and Evidence of Oxidative Stress in the Bronchoalveolar Fluid of Thoroughbred Colts during Race Training

Michelotto

Muehlmann

Zanatta

et al. 2010

Journal of Veterinary Internal Medicine

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“…PAF is a biologically active phospholipid [6] and one of the most potent proinflammatory mediator identified to date [7]. The correlation of PAF and asthmatic pulmonary inflammation is pointed out by the demonstration that PAF level is increased in asthmatic patients and decreased after immunotherapy [7][8][9].…”

Section: Enzymatic Pathwaysmentioning

confidence: 99%

“…In their study, Smiley [15] already suggested that PAF-like phospholipids may be generated under pathological conditions in which ROS play an essential role [15]. Currently, the participation of PAF-like molecules in many conditions is well established, such as in atherosclerosis and asthma [8,16].…”

Section: Non-enzymatic Pathwaysmentioning

confidence: 99%

“…In spite of being less potent than PAF, PAF-like lipids are generated in high quantities and in an uncontrolled manner, rendering their action important in inflammation [17]. In asthma, the most important immediate activities of PAF include leukocyte chemotaxis and activation, increase of vascular permeability, vasoconstriction, bronchoconstriction and bronchial hyperresponsiveness [5,7,8,18]. PAF also has long term effects [7] that determine the severity of asthma.…”

Section: Non-enzymatic Pathwaysmentioning

confidence: 99%

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The prevention of oxidative stress improve asthmatic inflammation

Zanatta¹,

Michelotto²,

Nishiyama³

2012

ABB

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Asthma is a disease characterized by airway chronic inflammation and bronchial hyperactivity, involving the imbalance of oxidative and antioxidative agents. There is an increased free radical generation and a decreased antioxidant enzyme activity, which correlate with the severity of the disease. The oxidative stress triggers specific physiopathological changes in the respiratory tract as a result of proinflammatory molecule formation, such as isoprostanes and PAF- like lipids. The synthesis of these mediators is dependent on the availability of lipid substrates, such as PUFAs, which are present in cell membranes. Therefore, lipid oxidation may have an important role in the perpetuation and amplification of the asthmatic inflammatory response. This article will make considerations about how oxidative stress contributes to asthma pathogenesis

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Dietary supplementation with soybean lecithin increases pulmonary PAF bioactivity in asthmatic rats

Cited by 12 publications

References 55 publications

Lung Tissue Damage Associated with Allergic Asthma in BALB/c Mice Could Be Controlled with a Single Injection of Mesenchymal Stem Cells from Human Bone Marrow up to 14 d After Transplantation

Lung Tissue Damage Associated with Allergic Asthma in BALB/c Mice Could Be Controlled with a Single Injection of Mesenchymal Stem Cells from Human Bone Marrow up to 14 d After Transplantation

Platelet-Activating Factor and Evidence of Oxidative Stress in the Bronchoalveolar Fluid of Thoroughbred Colts during Race Training

The prevention of oxidative stress improve asthmatic inflammation

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