Dietary supplementations modulate the physiological parameters, fatty acids profile and the growth of red claw crayfish (Cherax quadricarinatus)

Abstract

An optimal diet is an important factor for the proper growth and health of crustaceans. However, the regulation of antioxidant activity and non-specific immunity related to the consumption of feed additives has not been studied in RC-crayfish. Triplicate groups of 20 crayfish/tank (36.72 ± 0.70 g) fed with a basal diet and sixteen experimental diets that contained five feed additives with four grade levels (40, 160, 240 and 320 mg/kg vitamin E, 2, 4, 6 and 8 g/kg nucleotides, 2, 4, 6 and 8 g/kg Haematococcus pluvialis, 5, 10, 15 and 20 g/kg arachidonic acid and 2.5, 5, 10 and 15 g/kg yeast extract) on physiological parameters, fatty acids profile and growth of Cherax quadricarinatus for a period of 70 days by using orthogonal array method (L16 4 5 ). The results showed that the antioxidants activity in the haemolymph and hepatopancreas were both higher in crayfish fed with diets NO. 9 to 12 than others. Also, all the diets except diets NO. 13 to 16 showed lower free radicals contents than the control group. Similarly, significantly higher nonspecific immune parameters were observed in the hepatopancreas of crayfish supplementations than those fed a control diet. Biochemical parameters related to protein profile in haemolymph increased in diets NO. 9 to 12 and then decreased in control and diets NO. 13 to 16, while the highest biochemical parameters related to lipid profile except HDL-c contents in haemolymph were observed in crayfish fed the control diet. Fatty acid composition in the hepatopancreas, muscle and ovary of RC-crayfish was significantly influenced by using the combination of Vit E, NT, H. pluvialis and YP compared to the control group. Compared to all treatments, RCcrayfish fed with diets NO. 2 and 12 had significantly stimulated higher growth performance and feed utilisation. Overall, our results suggest that diets supplemented with Vit E level of 240 mg/kg, in combination with 8 g/kg NT, 4 g/kg, H. pluvialis, 5 g/kg ARA and 10 g/kg YP are the promising treatments to increase antioxidants activity, non-specific immune response, fatty acids composition and growth of RC-crayfish. However, high dietary supplementations level can reduce antioxidants activity, immunity and inhibit growth.

Natural zeolite for heavy metal, ammonia removal, and physiological responses in European sea bass (Dicentrarchus labrax) juveniles tanks with different densities

“…Biochemical parameters are commonly employed for assessing the physiological and overall health condition of aquatic organisms [81][82][83]. Elevated ALT and AST activities are acknowledged as markers reflecting the liver's health status [84][85][86]. The result of the present study significantly increases the albumin, cholesterol, and urea, as well as liver function enzymes such as AST and ALT, in the European sea bass exposed to the highest density.…”

Modulation of growth performance, feed utilization, and physiological traits in redbelly Tilapia (Tilapia zillii) through environmental and sex‐based interactions

“…Blood biochemical parameters are essential for assessing the overall well-being of fish, serving as key indicators of their physiological health. These parameters include vital serum components, particularly proteins that are crucial for both physiological and immunological functions (Peres et al, 2014;Shehata et al, 2023).…”

“…Blood biochemical parameters are essential for assessing the overall well‐being of fish, serving as key indicators of their physiological health. These parameters include vital serum components, particularly proteins that are crucial for both physiological and immunological functions (Peres et al, 2014; Shehata et al, 2023). They are widely employed to gauge the physiological status and general health of aquatic organisms, closely associated with factors such as dietary shifts and environmental conditions (Ferreira et al, 2011; van der Oost et al, 2003).…”

Evaluation of Dietary Essential Amino Acid Supplementation on Growth, Digestive Capacity, Antioxidant, and Intestine Health of the Juvenile Redclaw Crayfish, Cherax quadricarinatus

“…ALT and AST are always regarded as essential cellular and hepatopancreatic damage indicators [ 44 ]. When the serum ALT and AST activities increase, it implies that hepatopancreas function has been damaged [ 45 ]. The control group exhibited the highest transaminase values, suggesting that the dietary inclusion of lysine and threonine can mitigate hepatopancreatic damage.…”