Dietary trans–fatty acids alter adipocyte plasma membrane fatty acid composition and insulin sensitivity in rats

Ibrahim, Ahamed; Natarajan, Saravanan; Ghafoorunissa,

doi:10.1016/j.metabol.2004.08.019

Cited by 101 publications

(88 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, studies suggest that overexpression of LPL gene increases triglyceride level and insulin resistance in rat skeletal muscle and liver (29). Our previous observations showed that feeding both SFAs and TFAs increased triglyceride levels in plasma, suggesting increased lipolysis due to decreased adipocyte insulin sensitivity or decreased clearance of triglyceride from plasma (30). In the present study the increased levels of LPL mRNA in SFA diet-fed group could be extrapolated with increased adipocyte lipolysis and insulin resistance observed in our earlier study.…”

Section: Discussionsupporting

confidence: 77%

“…However, irrespective of LA levels in the diet, TFAs decreased insulin sensitivity to a greater extent than SFAs. The greater effects of TFAs on rat adipocyte insulin sensitivity could be attributed to decreased adipocyte plasma membrane fluidity (30). In the present study both dietary SFA and TFA were found to alter the expression of genes associated with insulin sensitivity in rat adipose tissue.…”

Section: Discussionsupporting

confidence: 47%

“…The findings that PPARg mRNA levels were not altered in SFA diet-fed rats suggest that the observed decrease in insulin sensitivity in SFA-fed rats may not be through the PPARg-related mechanism. Dietary TFAs decreased insulin sensitivity to a greater extent than SFAs (30), which could be due to decreased expression of PPARg.…”

Section: Discussionmentioning

confidence: 92%

“…The changes observed in the adipocyte plasma membrane phospholipid composition in both TFA-and SFA-fed groups were accompanied by decreased adipose tissue insulin sensitivity as evidenced by increased lipolysis, decreased antilipolysis and decreased glucose uptake in rat adipocytes. However, the effects of dietary TFAs on adipocyte insulin resistance were greater than the effects of dietary SFAs, which could be attributed to the decreased adipocyte plasma membrane fluidity in TFAfed rats (30).…”

Section: Introductionmentioning

confidence: 82%

See 3 more Smart Citations

Differential effects of dietary saturated and trans-fatty acids on expression of genes associated with insulin sensitivity in rat adipose tissue

et al. 2005

View full text Add to dashboard Cite

Objective: Trans-fatty acids (TFAs) are formed during partial hydrogenation of vegetable oils and are shown to be more atherogenic than saturated fatty acids (SFAs). Our previous study showed that dietary TFAs decrease adipose tissue insulin sensitivity to a greater extent than SFAs in rats. We hypothesized that the effects of these fatty acids on insulin sensitivity could be mediated through an alteration in gene expression. In the current study we have investigated the effects of dietary TFAs or SFAs on expression of genes associated with insulin sensitivity in rat adipose tissue. Design and methods: Male weanling Wistar/NIN rats were divided into four groups and fed one of the following diets containing 10% fat (g/100 g diet) differing only in the fatty acid composition for 3 months: control diet (3.7% linoleic acid (LA)), SFA diet (5% SFA), TFA diet 1 (1.5% TFA þ 1% LA) and TFA diet 2 (1.5% TFA þ 2% LA). The mRNA expression of peroxisome proliferator-activated receptor g (PPARg), lipoprotein lipase (LPL), glucose transporter-4 (GLUT4), resistin and adiponectin was analyzed in epididymal fat using RT-PCR. The effects of TFA were studied at two levels of LA to understand the beneficial effects of LA over the effects of TFA. Results: Both dietary SFA and TFA upregulated the mRNA levels of resistin. Dietary SFA downregulated adiponectin and GLUT4 and upregulated LPL, while TFA downregulated PPARg and LPL. The effects of dietary TFA on PPARg and resistin were not counteracted by increased LA (TFA diet 2). Conclusion: The effects of SFAs on the aforementioned genes except PPARg could be extrapolated towards decreased insulin sensitivity, while only the alteration in the mRNA levels of PPARg and resistin could be associated with insulin resistance in TFA-fed rats. These findings suggest that dietary SFAs and TFAs alter the expression of different genes associated with insulin sensitivity in adipose tissue. European Journal of Endocrinology 153 159-165

show abstract

Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 82%

See 2 more Smart Citations

Differential effects of dietary saturated and trans-fatty acids on expression of genes associated with insulin sensitivity in rat adipose tissue

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Axen et al (2003) fed rats a high-fat (60%E) diet with 17%E TFA or a low-fat (12%E) diet with no TFA and found that the high-fat, high-TFA diet increased visceral fat mass and liver lipid content and impaired glucose tolerance and insulin sensitivity. Ibrahim et al (2005) demonstrated that rats fed a diet with 3%E TFA had increased fasting insulin levels compared with control and that this increase was larger than for a diet with 10%E from SFA. Natarajan et al (2005) found that rats consuming 10%E TFA had decreased insulin-stimulated glucose transport compared with a diet with 10%E from SFA.…”

Section: Resultsmentioning

confidence: 91%

Health effects of trans-fatty acids: experimental and observational evidence

2009

View full text Add to dashboard Cite

Background/Objectives: Growing evidence indicates that trans-fatty acids (TFA) adversely affect cardiovascular health. As part of the World Health Organization (WHO) Scientific Update on TFA, we reviewed the evidence for effects of TFA consumption on coronary heart disease (CHD). Subjects/Methods: We searched Medline publications examining TFA consumption and CHD risk factors or outcomes, emphasizing results of studies in humans. We evaluated and synthesized evidence from both controlled feeding trials evaluating risk factors and long-term observational studies evaluating risk factors or clinical outcomes, each of which have complementary strengths and limitations, to enable the most robust and reliable inferences of effects. Results: The effects of TFA consumption on risk factors most consistently seen in both controlled trials and observational studies included adverse lipid effects (for example mlow-density lipoprotein cholesterol, khigh-density lipoprotein cholesterol (HDL-C), mtotal/HDL-C ratio), proinflammatory effects (for example mtumor necrosis factor-a activity, minterleukin-6, mC-reactive protein) and endothelial dysfunction. These effects were most prominent in comparison with cis unsaturated fats; adverse effects on total/HDL-C and endothelial function were also seen in comparison with saturated fatty acids (SFA). TFA may also worsen insulin sensitivity, particularly among individuals predisposed to insulin resistance; possible effects on weight gain and diabetes incidence require further confirmation. Five retrospective case-control studies and four prospective cohort studies demonstrated positive associations between TFA consumption and CHD events. A meta-analysis of prospective studies indicated 24, 20, 27 and 32% higher risk of myocardial infarction (MI) or CHD death for every 2% energy of TFA consumption isocalorically replacing carbohydrate, SFA, cis monounsaturated fatty acids and cis polyunsaturated fatty acids, respectively. The differential effects of specific TFA isomers may be important but are less well established. The available evidence indicates that trans-18:1 and particularly trans-18:2 isomers have stronger CHD effects than trans-16:1 isomers. The limited data suggest that the experimental effects of ruminant and industrial TFA are similar when consumed in similar quantities, but very few persons consume such high levels of ruminant TFA, and observational studies do not support adverse CHD effects of ruminant TFA in amounts actually consumed. Conclusions: Controlled trials and observational studies provide concordant evidence that consumption of TFA from partially hydrogenated oils adversely affects multiple cardiovascular risk factors and contributes significantly to increased risk of CHD events. The public health implications of ruminant TFA consumption appear much more limited. The effects of specific TFA isomers require further investigation.

show abstract

Foods for the prevention of diabetes: how do they work?

Thomas

Pfeiffer

2012

Diabetes Metabolism Res

View full text Add to dashboard Cite

With the diabetes epidemic reaching menacing proportions worldwide, there is an urgent need for the development of cost-efficient prevention strategies to be effective at the population level. Great potential in this direction lies in properly designed, large-scale dietary interventions. The macronutrient composition and the caloric content of our diet are major determinants of glucose homeostasis and there is a continuously growing list of foods, nutrients or individual compounds that have been associated with an increased or reduced incidence of diabetes mellitus. These include fat, carbohydrates, fibre, alcohol, polyphenols and other micronutrients or individual dietary compounds, which have been shown to either promote or prevent a progression towards a (pre-)diabetic state. This review aims to briefly summarize relevant epidemiological data linking foods to diabetes and to provide insights into the mechanisms through which these effects are mediated. These include improvement of insulin sensitivity or promotion of insulin resistance, regulation of inflammatory pathways, regulation of glucose transport and tissue glucose uptake, aggravation or attenuation of postprandial glycaemia/insulinaemia, interactions with hormonal responses and β-cell-dependent mechanisms.

show abstract

Dietary trans–fatty acids alter adipocyte plasma membrane fatty acid composition and insulin sensitivity in rats

Cited by 101 publications

References 42 publications

Differential effects of dietary saturated and trans-fatty acids on expression of genes associated with insulin sensitivity in rat adipose tissue

Differential effects of dietary saturated and trans-fatty acids on expression of genes associated with insulin sensitivity in rat adipose tissue

Health effects of trans-fatty acids: experimental and observational evidence

Foods for the prevention of diabetes: how do they work?

Contact Info

Product

Resources

About