Dietary Vegetable Powders Modulate Immune Homeostasis and Intestinal Microbiota in Mice

Abstract

As the largest immune organ of the human body, the intestine also plays a vital role in nutrient digestion and absorption. Some vegetables are considered to have improvement effects on the intestine. This experiment explored the effects of freeze-dried asparagus, broccoli and cabbage powder on the intestinal immune homeostasis and microflora of mice. Thirty-two mice were divided into four groups (n = 8), including control group (fed normal diet), asparagus group (fed normal diet with 5% asparagus power), broccoli group (fed normal diet with 5% broccoli power) and cabbage group (fed normal diet with 5% cabbage power). The experiment lasted 21 days. The results showed that the serum immunoglobulin concentration (IgA and IgM) and intestinal cytokine content (like IFN-γ and TNF-α) were increased after vegetable powder supplement. The experiment also detected that vegetable powder supplementation changed intestinal flora and their metabolites (short-chain fatty acid), which showed that the abundance of Lachnospiraceae and Bacteroides were decreased, while the abundance of Firmicutes and Lactobacillus as well as propionic acid and butyric acid contents were increased. Together, these vegetable powders, especially cabbage, changed the intestinal immune response and microbial activity of mice.

Determination of the Efficacy of Broccoli Sprouts Seeds on Growth, Blood Parameters, and Immunological Indicators in Experimentally Immunosuppressed Male Rats

“…Causing inhibition of cytokine production, which causes a decrease in the formation of interleukin 2 and 4 as well as TNF-a (Tumour interferon necrosis factor alpha), (IFN-γ) interferon gamma, factorwhich all cause a decrease in the activity of lymphocytes [33].The treatment using feeding on broccoli sprouted seed powder in treatments G3 and G4 caused an increase in IgG values, which were 0.48, 0.54mg/dl, respectively, while the IgA values were also high, reaching 0.44 and 0.47 mg/dl, respectively, as well as IgM Its values were significantly affected as they were 0.52 ,0.56mg/dl, respectively, to be significantly similar to their values in treatment G1. It is clear from the results that the use of broccoli sprouted seed powder led to a significant increase in the level of immunity compared with G2, and according to the results obtained and based on the experiment that was conducted to determine the effectiveness of broccoli buds in enhancing the immunity of laboratory animals, it is clear to us that this study agrees with the study that performed by [34], on the effect of broccoli sprouted on the immune system, as this study demonstrated an increase in the concentration of immunoglobulins in the blood after using the broccoli sprouted. It reached the extent of similarity with the immunological values in the serum of laboratory rats in the G1 treatment, and this indicates the effect of feeding in improving and raising the immunity of laboratory animals.…”

The effect of combined dietary supplementation of herbal additives on carcass traits, meat quality, immunity and cecal microbiota composition in Hungarian white geese

“…Intestine is the primary site for food digestion and nutrient absorption, whose microbiota composition is important for homeostasis maintenance ( Zou et al, 2021 ). In this study, we analyzed the microbiota of the caecum contents of Hungarian white geese by 16S rRNA sequencing.…”

Microbiota, IgA and Multiple Sclerosis

“…Metformin treatment partially restores IgA+ B cells in mice, and SIgA concentrations increase after bariatric surgery in obese patients [ 171 ]. Dietary restriction promotes IgA production [ 172 ] as dietary fiber [ 173 , 174 ] or polyphenol supplementation [ 175 ]. Dietary supplementation with bacterial components from Lactobacillus such as KDP [ 176 ] or EPS [ 177 ] also increases gut IgA production.…”