Dietary vitamin B12 regulates chemosensory receptor gene expression via the MEF2 transcription factor in <i>Caenorhabditis elegans</i>

McDonagh, Aja; Crew, Jeannette; Linden, Alexander M. van der

doi:10.1093/g3journal/jkac107

Cited by 4 publications

(3 citation statements)

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“…Furthermore, increased vitamin B12 availability protects C. elegans against exposure to the thiol-reducing agent dithiothreitol (43). These and other findings stress the central importance of dietary vitamin B12 in C. elegans metabolic processes and reveal roles of vitamin B12 in the regulation of growth, development, lifespan, chemosensory receptor gene expression, and responses to stress (11,38,44,45). The possibility of a role of vitamin B12 in MYb11-and MYb115-mediated protection against pathogen infection warrants further investigation.…”

Section: Microbiota Bacteria Elicit a Robust Proteomic Response Relat...mentioning

confidence: 70%

“…For example, vitamin B 12 was identified as the major metabolite accelerating C. elegans development and reproductive timing (35, 39). Moreover, vitamin B 12 can affect regulation of host growth, lifespan, chemosensory receptor gene expression, and responses to stress (10, 38, 67, 68). These and other findings stress the importance of microbe-derived vitamin B 12 in C. elegans metabolic processes, which should be considered when studying the effects of the (potentially vitamin B 12 -producing) C. elegans microbiota on host physiology.…”

Section: Discussionmentioning

confidence: 99%

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TheC. elegansproteome response to two protectivePseudomonasmutualists

Pees

Peters

Treitz

et al. 2023

Preprint

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The C. elegans natural microbiota isolates Pseudomonas lurida MYb11 and Pseudomonas fluorescens MYb115 protect the host against pathogens through distinct mechanisms. While P. lurida produces an antimicrobial compound and directly inhibits pathogen growth, P. fluorescens MYb115 protects the host without affecting pathogen growth. It is unknown how these two protective microbes affect host biological processes. We used a proteomics approach to elucidate the C. elegans response to MYb11 and MYb115. We found that both Pseudomonas isolates increase vitellogenin protein production in young adults, which confirms previous findings on the effect of microbiota on C. elegans reproductive timing. Moreover, the C. elegans responses to MYb11 and MYb115 exhibit common signatures with the response to other vitamin B12-producing bacteria, emphasizing the importance of vitamin B12 in C. elegans-microbe metabolic interactions. We further analyzed signatures in the C. elegans response specific to MYb11 or MYb115. We provide evidence for distinct modification in lipid metabolism by both mutualistic microbes. We could identify activation of host pathogen defense responses as MYb11-specific proteome signature and demonstrate that the intermediate filament protein IFB-2 is required for MYb115-mediated protection. These results indicate that MYb11 not only produces an antimicrobial compound, but also activates host antimicrobial defenses, which together might increase resistance to infection. In contrast, MYb115 affects host processes such as lipid metabolism and cytoskeleton dynamics, which might increase host tolerance to infection. Overall, this study provides new insights into the potential mechanisms underlying C. elegans microbiota-mediated protection from pathogen infection.

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Section: Microbiota Bacteria Elicit a Robust Proteomic Response Relat...mentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

TheC. elegansproteome response to two protectivePseudomonasmutualists

Pees

Peters

Treitz

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…For example, vitamin B 12 was identified as the major metabolite accelerating C. elegans development and reproductive timing ( 35 , 39 ). Moreover, vitamin B 12 can affect the regulation of host growth, lifespan, chemosensory receptor gene expression, and responses to stress ( 10 , 38 , 68 , 69 ). These and other findings stress the importance of microbial-derived vitamin B 12 in C. elegans metabolic processes, which should be considered when studying the effects of the (potentially vitamin B 12 -producing) C. elegans microbiota on host physiology.…”

Section: Discussionmentioning

confidence: 99%

The Caenorhabditis elegans proteome response to two protective Pseudomonas symbionts

Pees,

Peters,

Treitz

et al. 2024

mBio

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The Caenorhabditis elegans natural microbiota isolates Pseudomonas lurida MYb11 and Pseudomonas fluorescens MYb115 protect the host against pathogens through distinct mechanisms. While P. lurida produces an antimicrobial compound and directly inhibits pathogen growth, P. fluorescens MYb115 protects the host without affecting pathogen growth. It is unknown how these two protective microbes affect host biological processes. We used a proteomics approach to elucidate the C. elegans response to MYb11 and MYb115. We found that both Pseudomonas isolates increase vitellogenin protein production in young adults, which confirms previous findings on the effect of microbiota on C. elegans reproductive timing. Moreover, the C. elegans responses to MYb11 and MYb115 exhibit common signatures with the response to other vitamin B 12 -producing bacteria, emphasizing the importance of vitamin B 12 in C. elegans -microbe metabolic interactions. We further analyzed signatures in the C. elegans response specific to MYb11 or MYb115. We provide evidence for distinct modifications in lipid metabolism by both symbiotic microbes. We could identify the activation of host-pathogen defense responses as an MYb11-specific proteome signature and provide evidence that the intermediate filament protein IFB-2 is required for MYb115-mediated protection. These results indicate that MYb11 not only produces an antimicrobial compound but also activates host antimicrobial defenses, which together might increase resistance to infection. In contrast, MYb115 affects host processes such as lipid metabolism and cytoskeleton dynamics, which might increase host tolerance to infection. Overall, this study pinpoints proteins of interest that form the basis for additional exploration into the mechanisms underlying C. elegans microbiota-mediated protection from pathogen infection and other microbiota-mediated traits. IMPORTANCE Symbiotic bacteria can defend their host against pathogen infection. While some protective symbionts directly interact with pathogenic bacteria, other protective symbionts elicit a response in the host that improves its own pathogen defenses. To better understand how a host responds to protective symbionts, we examined which host proteins are affected by two protective Pseudomonas bacteria in the model nematode Caenorhabditis elegans . We found that the C. elegans response to its protective symbionts is manifold, which was reflected in changes in proteins that are involved in metabolism, the immune system, and cell structure. This study provides a foundation for exploring the contribution of the host response to symbiont-mediated protection from pathogen infection.

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