Diethylaminoethyl (DEAE) binding fraction from Taenia solium metacestode improves the neurocysticercosis serodiagnosis

Abstract

Neurocysticercosis (NC) is one of the most important diseases caused by parasites affecting the central nervous system. We fractionated by ion-exchange chromatography using diethylaminoethyl (DEAE)-sepharose resin the total saline extract (S) from Taenia solium metacestodes and evaluated obtained fractions (DEAE S1 and DEAE S2) by enzyme-linked immunosorbent assay (ELISA, n = 123) and immunoblotting (IB, n = 22) to detect human NC in serum. Diagnostic parameters were established by ROC and TG ROC curves for ELISA tests. IB was qualitatively analyzed. S and DEAE S1 presented sensitivity of 87. 5% and DEAE S2 90%. The best specificity was observed for DEAE S2 (90.4%). In IB, using DEAE S2 samples from NC patients presented bands of 20-25, 43-45, 55-50, 60-66, 82, 89, and 140 kDa. The great diagnostic parameters reached by DEAE S2 suggest the potential applicability of this fraction in NC immunodiagnosis.

Usefulness of gel filtration fraction as potential biomarker for neurocysticercosis in serum: towards a new diagnostic tool

“…Several studies have demonstrated the usefulness of the partial fractionation to obtain antigenic fractions with increased diagnostic parameters using other chromatographic approaches. Fractionation techniques [detergent Triton X-114 (Machado et al 2007; Gonçalves et al 2010), affinity for concanavalin A and jacalin (Nunes et al 2010; Oliveira et al 2010), ion-exchange chromatography with carboxymethyl sepharose and diethylaminoethyl sepharose (DEAE)] (Nunes et al 2013; Ribeiro et al 2014) lead to antigenic fractions with high potential in NCC immunodiagnosis using T. saginata or T. solium metacestodes.…”

Pediatric neurocysticercosis: current challenges and future prospects

“… 64 Diethylaminoethyl binding fraction from T. solium metacestode has been reported to improve the NCC serodiagnosis. 65 Recently, two magnetic immune-chromatographic tests for serologic detection of teniasis (based on rES33 antigen) and cysticercosis (based on the rT24H antigen) have shown high sensitivity and specificity in cases with ≥2 viable brain cysts. 66 A multiantigen print immunoassay is also being evaluated as a simple and effective tool to compare antibody responses to different cysticercosis and teniasis antigens and for rapid detection of T. solium cases.…”

Frações antigênicas de metacestódeos de Taenia saginata na neurocisticercose humana: análise proteômica e aplicação em plataformas imunodiagnósticas

“…Para diminuir a reatividade cruzada com outros parasitos, a utilização de extratos antigênicos fracionados, por técnicas cromatográficas, consistem em uma ferramenta importante para o diagnóstico da NC no desenvolvimento de testes sensíveis e específicos; a possibilidade de substituição do uso dos extratos antigênicos totais, que são misturas heterogêneas de proteínas, glicoproteínas e outros componentes. As principais vantagens dessas técnicas incluem produção rápida, fácil, de baixo custo e com alta eficiência (NUNES et al, 2013;RIBEIRO et al, 2014).…”