Diets containing crude glycerin damage the sperm characteristics and modify the testis histology of Nile tilapia broodstock

Mewes, Juliana Kasper; Meurer, Fábio; Tessaro, Lucélia; Buzzi, Alexandre Henrique; Syperreck, Mirna Adriane; Bombardelli, Robie Allan

doi:10.1016/j.aquaculture.2016.08.035

Cited by 22 publications

(15 citation statements)

References 48 publications

(65 reference statements)

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“…Taking into account the first sexual maturation, its administration did not influence growth, but impaired the male spermatogenesis process (Mewes et al 2016).…”

Section: Introductionmentioning

confidence: 94%

Effect of dietary crude glycerin on the productive performance of Nile tilapia fingerlings

Balen

Carneiro

Rossato

et al. 2020

An. Acad. Bras. Ciênc.

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“…Taking into account the first sexual maturation, its administration did not influence growth, but impaired the male spermatogenesis process (Mewes et al 2016).…”

Section: Introductionmentioning

confidence: 94%

Effect of dietary crude glycerin on the productive performance of Nile tilapia fingerlings

Balen

Carneiro

Rossato

et al. 2020

An. Acad. Bras. Ciênc.

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“…(2015); 5 Mewes et al . (2016): y = 115.1692–2.632x, r = 0.4683; 6 Balen et al . (2017); 7 Santos et al .…”

Section: Dietary Glycerol Inclusion and Its Influence On Plasma Glucosementioning

confidence: 99%

“…Although crude glycerine does not compromise growth, any level of dietary inclusion (4%, 8%, 12% and 16%) impairs the spermatogenesis process and the sperm characteristics of Nile tilapia breeders (Mewes et al, 2016).…”

Section: Fish Feed Glycerol Inclusion Levelsmentioning

confidence: 99%

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Use of glycerol in fish nutrition: a review

Mauerwerk

Zadinelo

Meurer

2020

Reviews in Aquaculture

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Crude glycerol and its purified and synthesized forms derived from biodiesel production are non‐toxic, biodegradable, biocompatible and versatile, and can be converted into various chemicals or products for biological use. Among the possible uses, there is its use in fish feeding, due to the chemical and nutritional composition, for being classified as energetic food and has low cost. The possibility of using glycerol in fish diets has been motivated by increasing maize prices, the excessive amount of glycerol produced and its processing costs for other purposes. Glycerol use in fish diet formulations is feasible, provided that certain factors be evaluated, such as digestibility and inclusion levels. In this context, this review discussed the main aspects that must be taken into account regarding glycerol use in fish diets, such as the characteristics and chemical composition of its categories, their fish metabolism pathways and their influence on fish plasma glucose. Crude glycerol or glycerine displays good potential for use in fish feed, as it contains adequate energy concentrations and can replace part of fish feed maize. However, it is evident that assessments concerning specific physiological phases, such as fish in reproductive phases, as well glycerol stability in water, are required.

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“…From these samples, aliquots were obtained for an evaluation of the percentage of morphologically normal sperm cells (94.12±7.25%). For this, 300 cells stained in Bengal Rose were evaluated under light microscopy on 40X objects and classified as normal and abnormal spermatozoa according to Mewes et al (2016).…”

Section: Seminal and Spermatic Analyzesmentioning

confidence: 99%

Rhamdia quelen's semen pre-cryopreservation processing and sperm viability time after thawing / Processo de pré-criopreservação de sêmen de Rhamdia quelen e tempo de viabilidade do esperma após o descongelamento

Krause¹,

Neumann²,

Pedreira³

et al. 2021

BJDV

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The study's aim was to evaluate the sperm movement in the semen of Rhamdia quelen, cryopreserved at different times of exposure to nitrogen vapor, besides different equilibration times and with different concentrations of methanol, and in thawed semen kept at 25ºC for different periods of time. Three distinct experiments were carried out. In the first experiment, fresh semen aliquots were diluted in a solution containing 5% D-Fructose, 5% milk powder (Nestle®, Ninho Fortificado®) and 10% methanol, filled into 0.25 mL straws and immediately kept in nitrogen vapor by 0,5; 1.0; 4.0; 8.0; 12.0 and 18.0h. After these times, the straws were transferred to the liquid nitrogen (-196°C). After 72 h, the semen was thawed by immersing the straws in water at (25 ° C) for 10 seconds. The sperm activation was evaluated by diluting the thawed semen in distilled water in the proportion of 1:250 (semen:activating solution; v/v). Motility and spermatic velocity in thawed semen were measured using the CASA system. In the second experiment, the fresh semen was diluted in solutions with glucose and powdered milk (Nestle®, Ninho Fortificado®) and 5.0; 7.5; 10.0; 12.5% methanol. Immediately after, it was filled into 0.25mL straws and kept at 25ºC by equilibrium times corresponding to 0, 20, 40 and 60 minutes. Subsequently, part of the straws was used for spermatic evaluation before cryopreservation and others were submitted to cryopreservation in nitrogen vapor for 30 minutes and after transferred to liquid nitrogen (-196°C). The processes of thawing and sperm evaluation were performed as described above. In the third experiment, the cryopreserved semen was thawed and kept at 25°C for 0.0; 7,5; 15,0; 22.5 and 30.0 minutes. After each time, the spermatic movement was evaluated as described above. The exposure time of straws to nitrogen vapor and the kept time of the thawed semen at 25ºC did not affect (p > 0.05) the sperm movement. On the other hand, methanol concentrations and equilibrium time (p < 0.05) influenced the spermatic movement interactively both, before and after cryopreservation. The best results were achieved when the semen was cryopreserved immediately after dilution. The cryopreservation of Rhamdia quelen semen can be successfully performed when the semen is diluted in a solution containing 5% D-Fructose, 5% milk powder and 11.66% of methanol and immediately filled into

show abstract

Diets containing crude glycerin damage the sperm characteristics and modify the testis histology of Nile tilapia broodstock

Cited by 22 publications

References 48 publications

Effect of dietary crude glycerin on the productive performance of Nile tilapia fingerlings

Effect of dietary crude glycerin on the productive performance of Nile tilapia fingerlings

Use of glycerol in fish nutrition: a review

Rhamdia quelen's semen pre-cryopreservation processing and sperm viability time after thawing / Processo de pré-criopreservação de sêmen de Rhamdia quelen e tempo de viabilidade do esperma após o descongelamento

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