Difference between orthostatic and march functional proteinuria by application of stress tolerance test and SDS–PAGE

Korneti, Petar; Tasić, Velibor; Jovanoska, Liljana K.; Korneti, Blagica

doi:10.1016/s0009-8981(00)00241-2

Cited by 4 publications

(4 citation statements)

References 4 publications

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“…Before the treatment of a child with proteinuria, functional (orthostatic) proteinuria should be excluded [ 2 ] [ 3 ] [ 4 ] [ 5 ]. It is characterised by excretion of proteins only in postural position.…”

Section: Introductionmentioning

confidence: 99%

The Spectrum of Kidney Diseases in Children Associated with Low Molecular Weight Proteinuria

Salihu¹,

Tosheska²,

Aluloska³

et al. 2018

Open Access Maced J Med Sci

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BACKGROUND:Proteinuria, in addition to haematuria, is the most important laboratory parameter in patients with nephro-urological diseases. Low molecular weight proteinuria (LMWP) is of particular importance because some diseases genetic and tubulointerstitial are diagnosed based on its presence.AIM:The purpose of this study is to describe the clinical features, the course and outcome of pediatric patients with a renal disease associated with LMWP.MATERIAL AND METHODS:This retrospective observational study included 250 pediatric patients with various kidney diseases in which the type of proteinuria was defined by 4-20% gradient gel sodium dodecyl sulphate polyacrylamide gel (SDS-PAG) electrophoresis.RESULTS:Isolated LMWP was detected in 12% of patients, while mixed glomerulotubular proteinuria was detected in 18% of patients. It was detected in all patients with the Dent-1/2 disease, Lowe’s syndrome and secondary Fanconi syndrome. Transient LMWP was also detected in a series of 12 patients with distal renal tubular acidosis. In patients with nephrotic syndrome, it was associated with corticoresistence and unfavourable clinical course.CONCLUSION:This study contributes to the understanding of the clinical spectrum of various kidney diseases associated with LMWP, their natural course, and the effect of therapy.

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Section: Introductionmentioning

confidence: 99%

The Spectrum of Kidney Diseases in Children Associated with Low Molecular Weight Proteinuria

Salihu¹,

Tosheska²,

Aluloska³

et al. 2018

Open Access Maced J Med Sci

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“…We have successfully used it for differentiation of glomerular from non-glomerular hematurias and for analysis of functional proteinurias (orthostatic, exercise induced proteinuria). [19,20,21] Analysis of the elepherograms enables pan-oramic investigation of various fractions according to their molecular weight and more subtle characterization of proteinuria (e.g. non selective glomerular with incomplete tubular proteinuria).…”

Section: Discussionmentioning

confidence: 99%

Low Molecular Weight Proteinuria in Children with Distal Renal Tubular Acidosis

Salihu

Tosheska²,

Cekovska³

et al. 2018

Prilozi

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Distal renal tubular acidosis (dRTA) (MIM #267300, #602722 and #179800) is a rare inherited tubulopathy characterized by the inability of the distal tubule to acidify the urine with consecutive systemic acidosis. The clinical features include polyuria, polydipsia, poor appetite, failure to thrive, short stature and rickets. Prominent biochemical features are hypokalemia, hypercalciuria and hypocitraturia. There are reports on patients who presented with unusual biochemical features such as low molecular proteinuria, hypophosphatemia, hypouricemia, generalized hyperaminioaciduria, hyperoxaluria and other making diagnostic confusion to the clinicians. In this work, we report on a series of 8 children with clinically, biochemically and genetically proven dRTA who present with low molecular proteinuria at the disease onset. With metabolic compensation of the disease, there was complete resolution of the low molecular weight protenuria and other proximal tubular abnormalities in all children. Late recognition of the disease with long standing hypokalemia and acidosis may result in abnormal expression and function of the transporters in the proximal tubules. Sodium dodecyl sulphate polyacrylamide gel electrophoeresis is an accurate method for detection and follow up of patients with low molecular weight proteinuria.

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“…Stress tolerance test and urine collections were performed as already described (5). Briefly, five urine samples were collected from each subject within 24 hours, depending on the level of physical activity: 1) early morning urine, 2) forenoon during ordinary daily activity, 3) immediately after physical exercise, 4) evening urine prior to sleep, and 5) early morning urine next day.…”

Section: Methodsmentioning

confidence: 99%

Stress Tolerance Test and SDS-PAGE for the Analysis of Urinary Proteins in Children and Youths

Tasić

Korneti²,

Gucev³

et al. 2001

Clinical Chemistry and Laboratory Medicine

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Excretion of urinary proteins (UP) is an important marker for the evaluation of patients with progressive renal disease. In order to analyze quantitative and qualitative variability of UP in relation to physical activity, we used standardized stress tolerance test and SDS-PAGE. Five urine samples were obtained from each patient at rest, during ordinary daily activity and after physical stress. Determination of total proteins was performed using Meulman's classic method with sulfosalicylic acid. UP were separated by ultrathin horizontal gradient SDS-PAGE according to Görg. There were 142 patients; 40 with poststreptococcal glomerulonephritis (PSGN), 11 with diabetes mellitus, 16 with chronic pyelonephritis and 75 who attended for investigation of asymptomatic proteinuria. Functional proteinuria was established in 42 subjects, who displayed maximal UP excretion during stress and the presence of apolipoprotein AI on SDS-PAGE. Children with PSGN showed no significant increase of UP during stress. Some children with diabetes mellitus (27%) and chronic pyelonephritis (47%) displayed microproteinuria or overt proteinuria after stress. Quantitative and qualitative changes in total UP excretion can be detected by stress tolerance test and SDS-PAGE. It remains to be seen whether stress tolerance test can identify children and youths who are at higher risk for disease progression.

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Difference between orthostatic and march functional proteinuria by application of stress tolerance test and SDS–PAGE

Cited by 4 publications

References 4 publications

The Spectrum of Kidney Diseases in Children Associated with Low Molecular Weight Proteinuria

The Spectrum of Kidney Diseases in Children Associated with Low Molecular Weight Proteinuria

Low Molecular Weight Proteinuria in Children with Distal Renal Tubular Acidosis

Stress Tolerance Test and SDS-PAGE for the Analysis of Urinary Proteins in Children and Youths

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