Difference in Binding of Long- and Medium-Chain Fatty Acids with Serum Albumin: The Role of Macromolecular Crowding Effect

Tiantian, Zhu; Zhang, Yan; Luo, Xing-An; Wang, Shen-Zhi; Jia, Ming-Qiang; Chen, Zhongxiu

doi:10.1021/acs.jafc.7b03548

Cited by 23 publications

(15 citation statements)

References 39 publications

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“…The crowding effect was also demonstrated for albumin with the help of Raman spectroscopy; a dense medium affects the strength of intramolecular hydrogen bonds in the BSA molecule, which forces the protein molecule to acquire a more compact structure [ 99 ]. Zhu et al showed that molecular crowding has an influence on the binding of saturated medium-chain FAs and unsaturated long-chain FAs to BSA [ 100 ]. Thus, it can be assumed that the characteristics of the binding and esterase activities of albumin in the bloodstream will differ from the constants measured in the “ideal solution” in in vitro and in silico experiments.…”

Section: Enzymatic Activity Of Albuminmentioning

confidence: 99%

Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties

Belinskaia

Voronina

Шмурак

et al. 2021

IJMS

161

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Being one of the main proteins in the human body and many animal species, albumin plays a decisive role in the transport of various ions—electrically neutral and charged molecules—and in maintaining the colloidal osmotic pressure of the blood. Albumin is able to bind to almost all known drugs, as well as many nutraceuticals and toxic substances, largely determining their pharmaco- and toxicokinetics. Albumin of humans and respective representatives in cattle and rodents have their own structural features that determine species differences in functional properties. However, albumin is not only passive, but also an active participant of pharmacokinetic and toxicokinetic processes, possessing a number of enzymatic activities. Numerous experiments have shown esterase or pseudoesterase activity of albumin towards a number of endogeneous and exogeneous esters. Due to the free thiol group of Cys34, albumin can serve as a trap for reactive oxygen and nitrogen species, thus participating in redox processes. Glycated albumin makes a significant contribution to the pathogenesis of diabetes and other diseases. The interaction of albumin with blood cells, blood vessels and tissue cells outside the vascular bed is of great importance. Interactions with endothelial glycocalyx and vascular endothelial cells largely determine the integrative role of albumin. This review considers the esterase, antioxidant, transporting and signaling properties of albumin, as well as its structural and functional modifications and their significance in the pathogenesis of certain diseases.

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Section: Enzymatic Activity Of Albuminmentioning

confidence: 99%

Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties

Belinskaia

Voronina

Шмурак

et al. 2021

IJMS

161

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“…[10,14,15]. Indeed, BSA is often used in research for its high similarity with human serum albumin (HSA) (approximately 76%) [16,17].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Fatty Acids and BSA Purity on Synthesis and Properties of Fluorescent Gold Nanoclusters

et al. 2020

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Fluorescent gold nanoclusters (AuNCs) are envisaged as a novel type of fluorophores. This work reports on the first comparative study investigating the effect of presence/absence/abundance of fatty acids (namely palmitic acid, PA) or other substances (like glycoproteins and globulins) in the protein (bovine serum albumin, BSA) on synthesis and properties of the final AuNCs. The most popular template (BSA) and microwave (MW)-assisted synthesis of AuNCs have been intentionally chosen. Our results clearly demonstrate that the fluorescent characteristics (i.e., fluorescence lifetime and quantum yield) are affected by the fatty acids and/or other substances. Importantly, the as-prepared AuNCs are biocompatible, as determined by Alamar Blue assay performed on Hep G2 cell line.

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“…Indeed, it has been shown that extent of FA binding to HSA and the number of relevant binding sites on the protein are modulated in a crowded environment. 53 Moreover, HSA being quite a large protein, it itself sweeps a considerable volume even in the native state, thereby increasing the chances of it getting perturbed in the presence of the crowders. While the domain movement is quite local in nature, translational diffusion studies as monitored using FCS cover a larger lengthscale over which the reporter diffuses in the mixed crowding environment, thereby allowing us to monitor the extent of complexity “on the offer” under such conditions.…”

Section: Introductionmentioning

confidence: 99%

Mixed Macromolecular Crowding: A Protein and Solvent Perspective

et al. 2018

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In the living cell, biomolecules perform their respective functions in the presence of not only one type of macromolecules but rather in the presence of various macromolecules with different shapes and sizes. In this study, we have investigated the effects of five single macromolecular crowding agents, Dextran 6, Dextran 40, Dextran 70, Ficoll 70, and PEG 8000 and their binary mixtures on the modulation in the domain separation of human serum albumin using a Förster resonance energy transfer-based approach and the translational mobility of a small fluorescent probe fluorescein isothiocyanate (FITC) using fluorescence correlation spectroscopy (FCS). Our observations suggest that mixed crowding induces greater cooperativity in the domain movement as compared to the components of the mixtures. Thermodynamic analyses of the same provide evidence of crossovers from enthalpy-based interactions to effects dominated by hard-sphere potential. When compared with those obtained for individual crowders, both domain movements and FITC diffusion studies show significant deviations from ideality, with an ideal solution being considered to be that arising from the sum of the contributions of those obtained in the presence of individual crowding agents. Considering the fact that domain movements are local (on the order of a few angstroms) in nature while translational movements span much larger lengthscales, our results imply that the observed deviation from simple additivity exists at several possible levels or lengthscales in such mixtures. Moreover, the nature and the type of deviation not only depend on the identities of the components of the crowder mixtures but are also influenced by the particular face of the serum protein (either the domain I–II or the domain II–III face) that the crowders interact with, thus providing further insights into the possible existence of microheterogeneities in such solutions.

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Difference in Binding of Long- and Medium-Chain Fatty Acids with Serum Albumin: The Role of Macromolecular Crowding Effect

Cited by 23 publications

References 39 publications

Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties

Serum Albumin in Health and Disease: Esterase, Antioxidant, Transporting and Signaling Properties

The Effect of Fatty Acids and BSA Purity on Synthesis and Properties of Fluorescent Gold Nanoclusters

Mixed Macromolecular Crowding: A Protein and Solvent Perspective

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