Abstract. A field study was carried out in an optometry clinic, aimed at assessing the role of perceived control and aversiveness of non-contact tonometry in intraocular pressure (IOP) reactivity to psychosocial stressors, and analyzing the covariation with cardiovascular and affective reactivity. Forty-four customers volunteered to participate in the study. Perceived control (self-efficacy and threat) was assessed at the onset. IOP, systolic and diastolic blood pressure, heart rate, affect, and aversiveness of the IOP measurement procedure were assessed throughout five phases with a mean duration for each phase of 9 min: arrival, optometry, baseline, stressor task (speech in public task), and recovery. The results suggest that IOP decreases over time and the stressor task induced a remarkable reactivity in all the physiological variables assessed. The interaction between self-efficacy and threat partially explains individual variability in IOP: a high threat combined with a high self-efficacy yielded higher reactivity in IOP or increased tonic values throughout the phases. The aversiveness of the measurement procedure did not affect IOP. Intraocular Pressure (IOP) is reactive to social stressors and perceived control partially explains individual variability. Cardiovascular and IOP reactivity are parallel phenomena but do not share a common regulatory mechanism.