Differences among Soil‐Inhabiting Microbial Communities in <i>Poa annua</i> Turf throughout the Growing Season

Beirn, Lisa A.; Hempfling, James W.; Schmid, Charles J.; Murphy, James A.; Clarke, Bruce B.; Crouch, Jo Anne

doi:10.2135/cropsci2016.06.0463

Cited by 20 publications

(21 citation statements)

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“…Overall, our study provides necessary baseline data on the composition of bacterial and fungal species on coolseason turfgrass golf courses and insight into the functions of those microbes on ecosystem health. All three golf courses were dominated by Proteobacteria, which corresponds with the results of Bartlett et al (2007), who found a high number of Gram-negative bacteria, and Beirn et al (2017), who found Proteobacteria to be the most dominant phylum on putting greens. We found a diverse microbial community containing organisms with many different functions, as found in previous turfgrass microbe studies.…”

Section: Discussionsupporting

confidence: 85%

Soil Microbial Communities on Roughs, Fairways, and Putting Greens of Cool‐Season Golf Courses

2019

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The microbial communities of golf courses provide many ecosystem functions and could be leveraged to provide better N fertility, biocontrol, and thatch degradation. However, little is known about soil bacterial and fungal communities on golf courses or how management affects them. We used metagenomics to identify the soil‐inhabiting bacteria and fungi among three management areas receiving few (roughs), moderate (fairways), and high (putting greens) inputs on three golf courses to determine how management intensity affects these organisms. Based on β diversity estimates, microbial communities on putting greens were more similar to each other than to the other areas of the golf courses. There were few significant differences in pathogens, biocontrol organisms, xenobiotic detoxifiers, or N‐cycling bacteria among management areas, suggesting soil turf bacteria are resilient to changes from management inputs. Fungal abundance, diversity, and pathogens were significantly affected by management areas, suggesting these organisms respond more drastically to management inputs. This is the first report using metagenomics to describe microbial communities on all three management areas of golf courses, including those managed organically. Our results provide insight on microbial community composition on golf courses, adding to a better understanding of perennial turf phytobiomes and how management intensity affects overall community composition.

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Section: Discussionsupporting

confidence: 85%

Soil Microbial Communities on Roughs, Fairways, and Putting Greens of Cool‐Season Golf Courses

2019

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“…The microbial biomass in warm-and cool-season turfgrass soils exhibited seasonal variations, with higher microbial biomass in May and December compared with September (Yao, Bowman, & Shi, 2011). Beirn et al (2016) observed an increase in archaeal and bacterial communities in the soil of an annual bluegrass (Poa annua L.) putting green over the course of 1 yr. More recently, Zhang et al (2017) observed higher bacterial and fungal diversity of soils with turf cover compared with soil from vacant lots. In turfgrass systems, turf managers use cultural and chemical management practices for reducing disease and maintaining vigorous turf, including biological control products such as Bacillus subtilis QST-713 (Rhapsody), with varying levels of success.…”

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confidence: 84%

“…Initial incubation steps were determined empirically to increase overall yields (data not shown). Soil and rhizosphere eDNA were extracted using the Qiagen DNeasy PowerSoil Kit (Qiagen) using methods as described by Beirn et al (2016).…”

Section: Sampling and Edna Extractionmentioning

confidence: 99%

“…Extracted eDNA was quantified using a Nanodrop 1000 (Thermo Fisher Scientific, Waltham, MA) and a Qubit fluorometer (Life Technologies). Based off Qubit quantification, all samples were diluted to 1.5 ng μl -1 for amplicon library generation, which was generated through a two-step PCR process as previously described by Beirn et al (2016). Briefly, bacterial 16S rDNA amplicons were generated using the Ba9F/Ba515Rmod1 primer pair (∼500 bp; Kittelmann et al, 2013;Weisburg, Barns, Pelletier, & Lane, 1991), whereas fungal ITS amplicons were generated using the ITS3_KYO2-F/ITS4-R primer pair (∼350 bp; Toju, Tanabe, Yamamoto, & Sato, 2012;White, Bruns, Lee, & Taylor, 1990).…”

Section: Amplicon Generation and Sequencing Preparationmentioning

confidence: 99%

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Elucidating the influence of resident seed and soil microbiota on the developing creeping bentgrass microbiome

Doherty

Crouch

Roberts

2020

Agrosystems Geosci & Env

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Characterization of the plant microbiome may allow for the development of diagnostic tools or improvements in plant health. To that end, this project sought to determine the influence that resident seed and soil microbiota exert on the developing creeping bentgrass (Agrostis stolonifera L. cv. 007) microbiome. A sterile growth environment was created in a laminar flow hood. Sterile 50 ml conical tubes were filled with autoclaved or non-autoclaved soil (85% sand and 15% peat) and seeded with 007 creeping bentgrass. Foliage and rhizosphere samples were taken at emergence, 2, 4, and 6 wk post-emergence, and soil samples were taken at the conclusion of the experiment. Amplicon sequencing libraries were generated from extracted environmental DNA, sequenced using an Illumina MiSeq, and analyzed in R. After quality control 3.32 × 10 7 sequences were grouped into 2,273 bacterial and 303 fungal amplicon sequence variants (ASVs). Bacterial and fungal ASVs were predominantly members of the Proteobacteria and Eurotiomycetes, respectively. Over the 6-wk sampling period the microbial communities were stable, with taxonomic shifts occurring mainly in low relative abundant taxa. Ordination of Bray-Curtis distance matrices revealed significant differences in community centroids between grass planted in autoclaved and non-autoclaved soil. However, taxonomic profiling showed these differences were due to shifts in taxa present at low relative abundances <.5%). Data show that the microbiota originating from the seed exerts only a minimal influence over the microbiome of developing creeping bentgrass compared to the soil microbiome.

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“…The importance of these microbial groups has motivated efforts to understand their ecology in global ecosystems and develop ways to manipulate their impacts on plants through management or plant breeding (Bakker, Manter, Sheflin, Weir, & Vivanco, ; Fierer, ; Walters et al, ). Despite the potential to leverage the microbial diversity in soil to influence plant growth, aside from work in turfgrass (Beirn et al, ; Crouch, Carter, Ismaiel, & Roberts, ), relatively little research has focused on plants that are cultivated for their ornamental traits.…”

Section: Introductionmentioning

confidence: 99%

Prokaryotic taxa play keystone roles in the soil microbiome associated with woody perennial plants in the genus Buxus

LeBlanc

Crouch

2019

Ecology and Evolution

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The microbiome associated with ornamental plants has largely been neglected, despite its potential for impacting plant health. This work characterized the composition, diversity, and microbial co‐associations in the soil microbiome associated with species and cultivars of plant in the genus Buxus (common name boxwood), a group of woody perennial shrubs commonly used in residential landscapes and found in native ecosystems. Soil was collected from 82 individual curated boxwood accessions at the U.S. National Arboretum National Boxwood Collection. Amplicon libraries targeting archaea, bacteria, and fungi were generated and sequenced using the Illumina MiSeq platform. Identification of individual sequence variants resulted in 275 archaeal, 15,580 bacterial, and 7,525 fungal taxa. Neither spatial distance among samples nor association with different types of boxwood were significant predictors of soil microbiome composition. However, archaeal and bacterial diversity was significantly different in soil from distinct types of boxwood. Co‐association networks indicated that archaea and bacteria show greater evidence of being keystone taxa than fungi. Overall, this work demonstrates the potential for targeting specific keystone taxa to shift the soil microbiome associated with these boxwood accessions and that planting different species or cultivars in the landscape may shift the diversity of prokaryotic microorganisms.

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Differences among Soil‐Inhabiting Microbial Communities in Poa annua Turf throughout the Growing Season

Cited by 20 publications

References 50 publications

Soil Microbial Communities on Roughs, Fairways, and Putting Greens of Cool‐Season Golf Courses

Soil Microbial Communities on Roughs, Fairways, and Putting Greens of Cool‐Season Golf Courses

Elucidating the influence of resident seed and soil microbiota on the developing creeping bentgrass microbiome

Prokaryotic taxa play keystone roles in the soil microbiome associated with woody perennial plants in the genus Buxus

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