Differences Between Indigenous Yeast Populations in Spontaneously Fermenting Musts From V. vinifera L. and V. labrusca L. Grapes Harvested in the Same Geographic Location

Abstract

Yeast communities associated with Vitis vinifera L. ecosystems have been widely characterized. Less is known, however, about yeast communities present in grapes and fermenting musts from Vitis non-vinifera ecosystems. Moreover, there are no comparative studies concerning yeast communities in grapes from V. vinifera L. and non-vinifera Vitis species in vineyards from a shared terroir. In this work, we have used a culture-dependent strategy, phenotypic analyses, and molecular genotyping, to study the most representative yeast species present in spontaneously fermenting musts of grapes harvested from neighboring V. vinifera L. (cv. Malbec) and V. labrusca L. (cv. Isabella) vineyards. Phenotypic analyses of H2S production, ethanol tolerance and carbon utilization, on randomly selected strains of each Hanseniaspora uvarum, Starmerella bacillaris and Saccharomyces cerevisiae strains, as well as microsatellite genotyping of S. cerevisiae isolates from each the Malbec and Isabella grape musts, suggest that V. vinifera L. and V. labrusca L. ecosystems could harbor different yeast strain populations. Thus, microbial communities in exotic Vitis species may offer opportunities to look for unique yeast strains that could not be present in conventional V. vinifera L. ecosystems.

Vitis species, vintage, and alcoholic fermentation do not drive population structure in Starmerella bacillaris (synonym Candida zemplinina) species

“…vinifera L.) or Isabella ( V . labrusca L.) grapes and from either 2015 or 2017 vintages (Raymond Eder et al, , ). The Portuguese population included 17 isolates from Azores Archipelago that were isolated from either V .…”

“…Isabella) grapes were harvested at their optimal ripeness stages from several vineyards in Colonia Caroya (vintages of March 2015 and 2017), located at 31°02′00″S/64°05′36″O and 491 m above sea level, in the province of Córdoba, Argentina. Separate spontaneous fermentations of a pool of destemmed and partially crushed Malbec and Isabella grapes were performed in a local winery (Raymond Eder, Conti, & Rosa, ). One hundred ten C .…”

“…One hundred ten C . zemplinina strains (Table ) were isolated from various initial sampling times (i.e., 0, 24, and 48 hr; Raymond Eder et al, , ). The isolates were identified by polymerase chain reaction (PCR)–restriction fragment length polymorphism and/or DNA sequencing of their 5.8S‐ITS (Internal Transcribed Spacer) rDNA regions as indicated (Raymond Eder et al, , ).…”

“… bReferences: (1) Raymond Eder et al (); (2) Raymond Eder et al (); (3) Drumonde‐Neves et al (); (4) Drumonde‐Neves et al ().…”

TheSSU1checkup, a rapid tool for detecting chromosomal rearrangements of theSaccharomyces cerevisiaechromosome XVI. An ecological and technological study on wine yeast

“…Strains were genotyped using fifteen polymorphic microsatellite loci (C3, C4, C5, C6, C8, C9, C11, SCAAT1, SCAAT2, SCAAT3, SCAAT5, SCAAT6, SCYOR267C, YKL172, YPL009) developed for estimating the genetic relationships among S. cerevisiae strains (Legras et al, 2007). Most of the strains were previously genotyped in our lab (Börlin et al , 2016; Raymond Eder, Conti and Rosa, 2018; Emilien Peltier et al , 2018;(Börlin, 2015) and the additional 82 strains were genotyped in this work using identical experimental conditions. Briefly, two multiplex PCRs were carried out in a final volume of 12.5 μL containing 6.25 μL of the Qiagen Multiplex PCR master mix (Qiagen, France), 1 μL of DNA template, and 1.94 μL of each mix, using the conditions previously reported (Peltier et al, 2018b).…”