Differences between retinal and choroidal microvascular endothelial cells (MVECs) under normal and hypoxic conditions

Brylla, Elke; Tscheudschilsuren, Gerelsul; Santos, Anna Navarette; Nieber, K; Spanel‐Borowski, Katharina; Aust, Gabriela

doi:10.1016/s0014-4835(03)00219-7

Cited by 24 publications

(20 citation statements)

References 42 publications

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“…Approximately 9% of genes were differentially expressed by unstimulated ECs, according to the significance analysis of microarrays (SAM) with false discovery rate equal to 5%. Differential expression of genes by retinal and choroidal EC has also been reported for bovine eyes studied by differential display RT-PCR, although the majority of transcripts were not identified [32]. In our study [31], gene ontology identified a relatively greater percentage of transcripts associated with the immune response in the group of genes expressed at higher levels in retinal EC.…”

Section: Ocular Vascular Endothelial Hetero-geneity: Evidence From Insupporting

confidence: 58%

“…In an earlier study using bovine eyes, Brylla et al [32] showed that transcript encoding vascular endothelial growth factor (VEGF) receptor, VEGF-R1, was relatively high in retinal EC, while expression of VEGFR-2 transcript was relatively low, in comparison to levels in choroidal EC. Gene expression for the Tie-2 ligand, angiopoetin (Ang)-2, was also higher in retinal EC, although Ang-1 was not differentially expressed.…”

Section: Ocular Vascular Endothelial Hetero-geneity: Evidence From Inmentioning

confidence: 99%

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Ocular Vascular Endothelial Heterogeneity

Bhattacharjee¹,

Smith²

2009

VDP

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Endothelial cells form the lining of the vasculature. Despite the continuity of this layer throughout the body, endothelial cells exhibit remarkable heterogeneity in structure, molecular composition and activity: between sites; and in response to different exposures. One important consequence of endothelial diversity is the localized nature of many vascular disorders. To date a limited number of studies have attempted to define unique phenotypic features of the different intraocular endothelial subpopulations, which include the endothelial cells of vascular beds in the iris, the choroid and the retina. Differences that distinguish endothelial cells in the circulations of the choroid and the retina, in particular, are believed to be major etiological factors controlling the specific involvement of the two tissues in some of the most common blinding diseases. Age-related macular degeneration involves choroid, and diabetic retinopathy and posterior uveitis are primarily diseases of the retina. Development of effective targeted therapies for these ocular disorders will require a detailed understanding of the heterogeneity of ocular endothelia. Our review summarizes the existing literature relating to diversity of the ocular endothelial cells. We highlight structural, metabolic and functional characteristics that distinguish intraoocular endothelial subtypes from each other and from extraocular endothelial cells, and we consider the implications of these differences for the design of novel biological therapeutics for eye diseases.

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Section: Ocular Vascular Endothelial Hetero-geneity: Evidence From Insupporting

confidence: 58%

Section: Ocular Vascular Endothelial Hetero-geneity: Evidence From Inmentioning

confidence: 99%

Ocular Vascular Endothelial Heterogeneity

Bhattacharjee¹,

Smith²

2009

VDP

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“…Production of ET-1 by LECs has been demonstrated to increase in response to TNFα stimulation in vitro [23]. This secretory response represents a useful parameter for investigating the endocrine status of isolated LECs in culture systems.…”

Section: Discussionmentioning

confidence: 95%

Effects of Storage and Passage of Bovine Luteal Endothelial Cells on Endothelin-1 and Prostaglandin F2.ALPHA. Production

Acosta

Yoshioka

Komiyama

et al. 2007

Journal of Reproduction and Development

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Abstract. To establish a storage system for isolated bovine luteal endothelial cells (LECs), we investigated the basal and tumor necrosis factor (TNF) α-stimulated production of endothelin-1 (ET-1) and prostaglandin (PG) F2α in unfrozen and frozen-thawed LECs until passage 10. LECs were obtained from developing corpora lutea (CL; days 5-7 of the estrous cycle) using enzymatic digestion and magnetic beads coated with lectin BS-1. The LECs were frozen at -80 C or further cultured and/ or passaged until passage 10 in DMEM/Ham's F-12 supplemented with 10% calf serum. The hormonal productions of unfrozen and frozen/thawed LECs were compared through passages 2-10. When both the unfrozen and frozen/thawed cells reached confluence, the culture medium was replaced with fresh medium containing 0.1% bovine serum albumin (BSA), and the cells were incubated with TNFα (50 ng/ml) for 12 h. The basal productions of ET-1 and PGF2α by the unfrozen and frozen/thawed LECs were similar at passage 2. The basal production of PGF2α by LECs was not altered by passage and storage at -80 C, whereas the basal production of ET-1 decreased from passage 2 and 3 to passage 4 in the unfrozen LECs and from passage 2 to passage 3 in the frozen/thawed LECs. However, production of ET-1 by the unfrozen and frozen/thawed LECs was similar between passages 4-10 and passages 3-10, respectively. Exposure of LECs to TNFα increased (P<0.05) ET-1 and PGF2α production by the unfrozen and frozen-thawed LECs in all passages examined. Thus, LECs obtained from developing CLs and stored until passage 10 can be used for study of the physiology of LECs in vitro. Key words: Bovine, Corpus luteum, Cytokines, Endothelin-1, Endothelium, Prostaglandins (J. Reprod. Dev. 53: [473][474][475][476][477][478][479][480] 2007) he corpus luteum (CL) is a complex organ that mainly consists of endothelial cells (ECs), steroidogenic cells, fibroblasts, and immune cells [1,2]. After ovulation, thecal microvessels invade the granulosal cell layer, and ECs rapidly proliferate in the early CL constituting more than 50% of the total cells in the mid-cycle CL [3][4][5]. The dense vascular network established at mid-cycle is essential for adequate CL function. It also enables an intricate cross-talk between steroidogenic and luteal endothelial cells (LECs) [6]. The products of LECs are directly associated with the capacity of

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“…The CRECs line was originally derived from a rhesus macaque fetus. 26 Although the origin of the cell line could not be assigned to choroid or retina, results from a published report 27,28 showed that the expressions of two VEGF receptors, fms-like tyrosine kinase (Flt-1) and kinase insert domain receptor (KDR) 27 were the same as in bovine choroidal endothelial cells but different from those expressed in bovine retinal microvascular endothelial cells, 28 suggesting that CRECs have characteristics more similar to those of choroid endothelial cells than of retinal vascular endothelial cells. CRECs have been confirmed as a useful cell line for studying the mechanisms of AMD pathogenesis.…”

mentioning

confidence: 99%