Background: Single eyelid is a unique characteristic in East and SouthEast Asian. Narrow palpebral fissure with compensatory elevation of eyebrows is common in this group. This study aims to demonstrate the correlation between superior visual field defect and MRD1 without lifting of the overhanging skin (MRD1w) in single eyelid population. Methods: Fifty healthy single eyelid volunteers were included. Outcome measurement included visual acuity, MRD1w, MRD2 and superior 64-point screening test visual field. MRD1w measurement and visual field testing with suppression of frontalis muscle were also performed. Results: A total of 38 visual field results from the right eye of 38 subjects were analyzed. Twelve fields were excluded due to high false negative error results. There were 22 female and 16 male subjects. The mean MRD1w was 2.25 +/-0.99 mm and the superior visual field at the 90-degree vertical meridian was 45.34o +/-4.88o. There is a positive correlation of MRD1w and superior 90-degree vertical meridian visual field (r = 0.64, p < 0.0001). The mean superior visual field with frontalis suppression was significantly lower when compared to subject natural brow position (40.17o +/-7.84o, p < 0.0001). Conclusions: Superior visual field defect in single eyelid population is correlated to MRD1w value. Compensatory brow elevation might alleviate symptoms but surgical correction for functional purpose should also be considered.