Differences between the Leaf Mycobiome of Coffea arabica and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content

Sousa, Leandro Pio de; Filho, Oliveiro Guerreiro; Mondego, Jorge Maurı́cio Costa

doi:10.3390/microorganisms9112296

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…Coffee is one of the agricultural export goods that generates significant foreign exchange to producing countries in Africa, Latin America, and Asia, supporting the livelihoods of approximately 125 million farmers (de Sousa et al, 2021). In Chiapas, high production coffee trees adapted to local climatic conditions have been observed, with the potential to increase coffee production per unit area.…”

Section: Introductionmentioning

confidence: 99%

“…In this regard, Lu et al (2022) reported 648 fungi species in coffee plants, 46 % of which are pathogens and 22 % are endophytes. In Coffea species, Trichoderma, Mycosphaerella, Cladosporium, and Xylaria are mainly reported as endophyte genera; and Fusarium, Alternaria, and Phoma as pathogens (de Sousa et al, 2021). In in vitro culture, these microorganisms cause contamination.…”

Section: Introductionmentioning

confidence: 99%

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FIELD PHYTOPROTECTION OF Coffea arabica MOTHER PLANTS, DISINFECTION AND CALLOGENESIS INDUCTION

Dávila

Ruiz-Sesma

Lecona-Guzmán

et al. 2023

agro

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In Chiapas, Mexico, there are high-yielding coffee plants adapted to the local climatic conditions. The presence of microorganisms in the explants makes developing protocols for cloning these genotypes in vitro difficult. The objective of this work was to evaluate how field and in vitro microorganism management affected asepsis and the induction of callogenesis in leaf explants of agronomically important coffee plants. Drought-tolerant genotypes were obtained from aseptic explants and cell cultures using a participatory study, agronomic, and in vitro techniques. Five accessions (ITTGj 1-5) were selected from a 21-year-old Borbon cultivar. The present study was conducted over two flowering cycles, evaluating the effect of field management on explant asepsis on the first and the callogenic response caused by the culture medium, the transport solution, and the genotype, on the second. Sixty days before explant collection, biweekly applications of calcium polysulfide (10 % v v-1), copper oxychloride (2 g L-1), and Zingiber officinale extract (20 g L-1) reduced fungal contamination by 100 % and bacterial contamination by 90 %. Field explant transportation in 200 mg L-1 citric acid and ascorbic acid solution reduced oxidation in explants and in vitro cultures. Proembryogenic friable corns were formed with the combination of 4.4 µM BAP (6-benzylaminopurine) and 7.25 µM 2,4-D (2,4-dichlorophenoxyacetic acid) at 45 days of culture, with genotype four showing the best response. Field management was effective for the aseptic establishment of Coffea arabica cultures in vitro, and the combination of BAP and 2,4-D used was an appropriate growth regulator for the induction of callogenesis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

FIELD PHYTOPROTECTION OF Coffea arabica MOTHER PLANTS, DISINFECTION AND CALLOGENESIS INDUCTION

Dávila

Ruiz-Sesma

Lecona-Guzmán

et al. 2023

agro

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Cryptococcus neoformans: plant–microbe interactions and ecology

Hallas-Møller,

Burow,

Henrissat

et al. 2024

Trends in Microbiology

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Leaf surface microbiota transplantation confers resistance to coffee leaf rust in susceptible Coffea arabica

de Sousa,

Mondego

2024

FEMS Microbiology Ecology

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Coffee leaf rust, caused by the fungus Hemileia vastatrix, became a major concern for coffee-producing countries. Additionally, there is an increase in the resistance of certain races of the fungus to fungicides and breeding cultivars, making producers to use alternative control methods. In this work, we transplanted the leaf surface microbiota of rust-resistant coffee species (Coffea racemosa and Coffea stenophylla) to Coffea arabica and tested whether the new microbiota would be able to minimize the damage caused by H. vastatrix. It was seen that the transplant was successful in controlling rust, especially from C. stenophylla, but the protection depended on the concentration of the microbiota. Certain fungi such as Acrocalymma, Bipolaris, Didymella, Nigrospora, Setophaeosphaeria, Simplicillium, Stagonospora, Torula and bacteria such as Chryseobacterium, Sphingobium and especially Enterobacter and have their populations increased and may be related to the antagonism seen against H. vastatrix. Interestingly, relative population of bacteria from genera Pantoea, Methylobacterium and Sphingomonas decreased after transplant, suggesting a positive interaction between them and H. vastatrix development. Our findings may help to better understand the role of the microbiota in coffee leaf rust as well as help to optimize the development of biocontrol agents.

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Differences between the Leaf Mycobiome of Coffea arabica and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content

Cited by 4 publications

References 36 publications

FIELD PHYTOPROTECTION OF Coffea arabica MOTHER PLANTS, DISINFECTION AND CALLOGENESIS INDUCTION

FIELD PHYTOPROTECTION OF Coffea arabica MOTHER PLANTS, DISINFECTION AND CALLOGENESIS INDUCTION

Cryptococcus neoformans: plant–microbe interactions and ecology

Leaf surface microbiota transplantation confers resistance to coffee leaf rust in susceptible Coffea arabica

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