Differences in antioxidant potential of chalcones in human serum: In vitro study

Janković, Tamara; Turkovic, Nemanja; Kotur‐Stevuljević, Jelena; Vujić, Zorica; Ivković, Branka

doi:10.1016/j.cbi.2020.109084

Cited by 9 publications

(4 citation statements)

References 27 publications

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“…Serum samples were pooled and aliquoted, then frozen at −80 °C and used for redox status analytics no more than 2 weeks after the collection. 19…”

Section: Methodsmentioning

confidence: 99%

“…Serum samples were pooled and aliquoted, then frozen at −80 °C and used for redox status analytics no more than 2 weeks after the collection. 19 The test samples were prepared by diluting each emulsion (S, S Q , S DHQ , and S DHQβC ) to 1% in PG, and dissolving antioxidants (Q and DHQ) to 0.005% in PG. Serum volume was 450 μL and the added volume of each previously prepared test sample was 50 μL (1:10 ratio), ie, the exogenous addition did not exceed 10% of the total sample volume, which is of the utmost importance to samples' bio-matrix quality provision.…”

Section: Methodsmentioning

confidence: 99%

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Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment

Martić

Kotur‐Stevuljević

Malenović

et al. 2022

Natural Product Communications

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Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extracellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The physicochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emulsion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.

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“…Serum samples were pooled and aliquoted, then frozen at −80 °C and used for redox status analytics no more than 2 weeks after the collection. 19…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment

Martić

Kotur‐Stevuljević

Malenović

et al. 2022

Natural Product Communications

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“…The pharmacological profile of chalcones ( Salehi et al, 2021 ) is wide and interesting and they are potentially useful for the development of therapeutic agents against cancer ( Ouyang et al, 2021 ), infectious diseases ( Mahapatra et al, 2015 ; Duran et al, 2021 ; Pozzetti et al, 2022 ), obesity, neurological/neurodegenerative disorders ( Cho et al, 2011 ; Thapa et al, 2021 ), inflammatory-related syndromes ( Mahapatra et al, 2017 ), allergic illnesses ( Yamamoto et al, 2004 ; Iwamura et al, 2010 ) etc. In addition, antioxidant effects of chalcones have been demonstrated ( Janković et al, 2020 ) conferring to them the status of privileged scaffolds. In fact, chalcone-based molecules such as metochalcone and sofalcone are used in clinical practice as choleretic drugs and anti-ulcer agents, respectively, also establishing gastroprotection in patients suffering from Helicobacter pylori ( Higuchi et al, 2010 ; Gomes et al, 2017c ).…”

Section: Introductionmentioning

confidence: 99%

The green chemistry of chalcones: Valuable sources of privileged core structures for drug discovery

Marotta

Rossi²,

Ibba³

et al. 2022

Front. Chem.

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The sustainable use of resources is essential in all production areas, including pharmaceuticals. However, the aspect of sustainability needs to be taken into consideration not only in the production phase, but during the whole medicinal chemistry drug discovery trajectory. The continuous progress in the fields of green chemistry and the use of artificial intelligence are contributing to the speed and effectiveness of a more sustainable drug discovery pipeline. In this light, here we review the most recent sustainable and green synthetic approaches used for the preparation and derivatization of chalcones, an important class of privileged structures and building blocks used for the preparation of new biologically active compounds with a broad spectrum of potential therapeutic applications. The literature here reported has been retrieved from the SciFinder database using the term “chalcone” as a keyword and filtering the results applying the concept: “green chemistry”, and from the Reaxys database using the keywords “chalcone” and “green”. For both databases the time-frame was 2017–2022. References were manually selected based on relevance.

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“…Therefore, antioxidant therapies have been considered viable treatment options for male infertility since they are able to restore the proper balance between free radicals and antioxidants in the reproductive cells [ 30 ]. Some chalcones have been reported to have antioxidant properties [ 11 , 15 , 31 , 32 , 33 ]. This activity is highly influenced by the structure of the two aromatic rings in the backbone [ 34 ] and by the number and position of the hydroxy group, methoxy group, and other groups in the A and B rings [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Different Strategies to Attenuate the Toxic Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells

et al. 2022

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Zinc oxide nanoparticles (ZnO NPs) are one of the most used nanoparticles due to their unique physicochemical and biological properties. There is, however, a growing concern about their negative impact on male reproductive health. Therefore, in the present study, two different strategies were used to evaluate the recovery ability of spermatogonia cells from the first stage of spermatogenesis (GC-1 spg cell line) after being exposed to a cytotoxic concentration of ZnO NPs (20 µg/mL) for two different short time periods, 6 and 12 h. The first strategy was to let the GC-1 cells recover after ZnO NPs exposure in a ZnO NPs-free medium for 4 days. At this phase, cell viability assays were performed to evaluate whether this period was long enough to allow for cell recovery. Exposure to ZnO NPs for 6 h and 12 h induced a decrease in viability of 25% and 41%, respectively. However, the recovery period allowed for an increase in cell viability from 16% to 25% to values as high as 91% and 84%. These results strongly suggest that GC-1 cells recover, but not completely, given that the cell viability does not reach 100%. Additionally, the impact of a synthetic chalcone (E)-3-(2,6-dichlorophenyl)-1-(2-hydroxyphenyl)prop-2-en-1-one (1) to counteract the reproductive toxicity of ZnO NPs was investigated. Different concentrations of chalcone 1 (0–12.5 µM) were used before and during exposure of GC-1 cells to ZnO NPs to mitigate the damage induced by NPs. The protective ability of this compound was evaluated through viability assays, levels of DNA damage, and cytoskeleton dynamics (evaluating the acetylated α-tubulin and β-actin protein levels). The results indicated that the tested concentrations of chalcone 1 can attenuate the genotoxicity induced by ZnO NPs for shorter exposure periods (6 h). Chalcone 1 supplementation also increased cell viability and stabilized the microtubules. However, the antioxidant potential of this compound remains to be elucidated. In conclusion, this work addressed the main cytotoxic effects of ZnO NPs on a spermatogonia cell line and analyzed two different strategies to mitigate this damage, which represent a significant contribution to the field of male fertility.

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Differences in antioxidant potential of chalcones in human serum: In vitro study

Cited by 9 publications

References 27 publications

Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment

Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment

The green chemistry of chalcones: Valuable sources of privileged core structures for drug discovery

Different Strategies to Attenuate the Toxic Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells

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