Introduction: To assess the type 2 diabetes mellitus prevalence and risk factors associated, among patients, at Franceville Amissa Bongo regional Hospital Gabon, A prospective and cross-sectional study was carried out between June and September 2023. Materials and Methods: Blood glucose was measured using blood drawn from each participant, according to American Diabetes Association standards. As elsewhere, correlations between type 2 diabetes mellitus prevalence and the variables obtained, were carried out using R software version 3.6.1. Odds ratios were calculated within a 95% interval, and results were considered significant when the p-value ≤ 0, 05. Results: Blood samples from 328 people, with a mean age of 48.24 ± 13.8 years, were collected for this study. The type 2 diabetes mellitus prevalence was 13.72% (95% CI: [0.10- 0.18]), (n=45). After univariate analysis, adjustment of variables by binary multivariate logistic regression, indicated female gender (Ajusted OR = 0.53; 95% CI [1.52; 39.41] p = 0.014*), cohabiting (Ajusted OR = 92.73 ; 95% CI [2.9; 29.05] p = 0.010*), inactive (Ajusted OR = 9.17; 95% CI: [1.11; 75.05] p = 0.040*), overweight/obese (Ajusted OR = 0. 017; 95% CI: [0.002; 0.18] p = 0.010*), a family history of type 2 diabetes mellitus (Ajusted OR = 50.13; 95% CI [8.87; 283.50] p = 0.000*) and high triglyceride levels (Ajusted OR = 0.03; 95% CI [0.003; 0.36] p = 0.005*), as type 2 diabetes mellitus potential risk factors, in the present study. Conclusion: The type 2 diabetes mellitus prevalence in the study population was high. Prevention and control of this disease through early detection are essential.