Differences in composition and fluidity of intestinal microvillus membrane vesicles prepared by different methods

Bjorkman, David J.; Brigham, Earl J.

doi:10.1016/0006-291x(90)92110-l

Cited by 12 publications

(3 citation statements)

References 33 publications

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“…In this study the "canalicular" fraction was found to be less fluid than the "sinusoidal" fraction, yet the difference was smaller than that reported with different methods of LPM subfractionation (45, 47). It is worth mentioning, however, that fluidity and biochemical measurements can be largely influenced by the method involved in membrane subfractionation (48).…”

Section: Discussionmentioning

confidence: 99%

Improvement of estradiol 17β-D-glucuronide cholestasis by intravenous administration of dimethylethanolamine in the rat

Alvaro¹,

Angélico²,

Cantàfora³

et al. 1991

Hepatology

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The intravenous administration of dimethylethanolamine in the rat promotes a selective enrichment of liver membranes with polyunsaturated phosphatidylcholines. The effect of dimethylethanolamine pretreatment on cholestasis induced by estradiol 17 beta-D-glucuronide, a potent cholestatic agent, was assessed in this study. Dimethylethanolamine, dissolved in sodium-taurocholate was infused intravenously (0.3 mg/kg/min) for 15 hr. One group of control rats (estradiol 17 beta-D-glucuronide controls) received the bile salt only. An estradiol 17 beta-D-glucuronide bolus was then injected intravenously (10.4 mg/kg) into dimethylethanolamine-pretreated and estradiol 17 beta-D-control rats, and its effect on bile flow and biliary lipid secretion was compared for 3 hr. The estradiol 17 beta-D-glucuronide inhibitory effect on bile flow and biliary lipid secretion was significantly antagonized by dimethylethanolamine pretreatment. The maximum inhibition of bile flow was found 30 min after estradiol 17 beta-D-glucuronide administration, when it decreased from 3.5 +/- 0.4 microliters/min/100 gm (basal) to 0.9 +/- 0.3 microliters/min/100 gm in estradiol 17 beta-D-glucuronide controls, whereas in dimethylethanolamine-pretreated rats this decreased only from 3.2 +/- 0.4 (basal) to 2.3 +/- 0.4 microliters/min/100 gm. Bile flow and the biliary secretion of cholesterol, phosphatidylcholine and bile salts were significantly higher in the dimethylethanolamine-pretreated rats than in estradiol 17 beta-D-glucuronide controls (p less than 0.02) during the cholestatic phase. The inhibitory effect of estradiol 17 beta-D-glucuronide on bile flow was associated with a marked decrease of membrane fluidity (p less than 0.001) assessed by 1,6-diphenyl-1,3,5-hexatriene fluorescence anisotropy and with a cholesterol enrichment of microsomes, sinusoidal and canalicular liver plasma membranes and inhibition of sinusoidal Na+,K(+)-ATPase activity (p less than 0.05). These membrane alterations persisted 180 min after estradiol 17 beta-D-glucuronide administration despite complete normalization of bile flow. Dimethylethanolamine pretreatment significantly counteracted the reduction of membrane fluidity (p less than 0.001), the cholesterol enrichment and the inhibition of Na+,K(+)-ATPase (p less than 0.05) promoted by estradiol 17 beta-D-glucuronide administration in all membrane subfractions 30 and 180 min after administration. In addition, dimethylethanolamine-pretreated rats had more polyunsaturated fatty acids in membrane phosphatidylcholine with respect to the control groups. Dilatation of canaliculi and loss of microvilli were evident in estradiol 17 beta-D-glucuronide controls 180 min after estradiol 17 beta-D-glucuronide administration.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Discussionmentioning

confidence: 99%

Improvement of estradiol 17β-D-glucuronide cholestasis by intravenous administration of dimethylethanolamine in the rat

Alvaro¹,

Angélico²,

Cantàfora³

et al. 1991

Hepatology

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“…BBM prepared from rodent intestine using Ca 2+ or Mg 2+ precipitation were found to differ in their lipid composition, 9 size and shape, intramembrane particle disruption, and protein composition 10 . Phospholipid composition and the static and dynamic components of fluidity were also different 11 …”

Section: Introductionmentioning

confidence: 99%

Simple method of preparation of rat colonocyte apical membranes using polyethylene glycol precipitation

Ramamoorthy

Balasubramanian

2003

J of Gastro and Hepatol

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“…BBMV prepared from rodent intestine by Ca 2ϩ and Mg 2ϩ precipitation differ in their lipid composition (7), size and shape, intramembrane particle distribution, and protein composition (8). Phospholipid composition as well as static and dynamic components of fluidity were also found to differ (9). Our earlier work has shown that BBMV prepared by Ca 2ϩ or Mg 2ϩ precipitation from rat intestine differ in their purity and lipid composition, whereas BBMV prepared from monkey intestine using these cations are similar (7).…”

mentioning

confidence: 97%

A Novel Method of Preparation of Small Intestinal Brush Border Membrane Vesicles by Polyethylene Glycol Precipitation

Ramamoorthy

Balasubramanian

2001

Analytical Biochemistry

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Differences in composition and fluidity of intestinal microvillus membrane vesicles prepared by different methods

Cited by 12 publications

References 33 publications

Improvement of estradiol 17β-D-glucuronide cholestasis by intravenous administration of dimethylethanolamine in the rat

Improvement of estradiol 17β-D-glucuronide cholestasis by intravenous administration of dimethylethanolamine in the rat

Simple method of preparation of rat colonocyte apical membranes using polyethylene glycol precipitation

A Novel Method of Preparation of Small Intestinal Brush Border Membrane Vesicles by Polyethylene Glycol Precipitation

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