Differences in DNA methylation, DNA structure and embryogenesis-related gene expression between embryogenic and non embryogenic lines of Pinus radiata D. don

Bravo, Soraya; Bertin, A.; Turner, A. Richard; Sepúlveda, Francisco; Jopia, Paz; Parra, M.; Castillo, Rosario; Hasbún, Rodrigo

doi:10.1007/s11240-017-1242-3

Cited by 24 publications

(24 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…WOX2 , the WUS homolog gene, has been proposed as an embryogenic potential marker for some Picea and Larix species, since it is not present in non-embryogenic lines (Palovaara and Hakman, 2008; Park et al, 2010). In contrast, WOX2 expression was detected in both embryogenic and non-embryogenic lines in P. radiata , although transcript levels were lower in the latter (Bravo et al, 2017). Maritime pine WOX gene family has been studied recently (Alvarez et al, 2018) and WOX2 expression has been shown to be highest at early embryogenic stages and decreased along the maturation process being lower in mature somatic embryos which agree with our WOX2 expression profile sampled at 0, 7, and 12 weeks in SK lines.…”

Section: Discussionmentioning

confidence: 89%

“…Although in some conifer species somatic embryos maturation can be achieved without ABA, those derived from ABA-supplemented medium displayed a coordinated growth, better morphology and the concomitant accumulation of lipids and storage proteins, while these features were lacking in embryos developed in the absence of ABA (Klimaszewska et al, 2016). Comparative studies on zygotic and somatic embryos have been performed in several conifer species regarding gene expression (Lara-Chavez et al, 2012; Morel et al, 2014b; Bravo et al, 2017), hormonal content (von Aderkas et al, 2001) and storage proteins(Tereso et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

et al. 2019

View full text Add to dashboard Cite

Maritime pine ( Pinus pinaster Aiton) is a coniferous native of the Mediterranean basin. Because of its adaptability to a wide range of environmental conditions, the species have become a model for studies in coniferous forest management and functional genomics. Somatic embryogenesis (SE) has been so far, the preferred biotechnological strategy for maritime pine breeding programs initiated at the middle-end of the 20th century. To overcome the limitations of the induction and maturation phases in maritime pine SE, we analyzed the possible maternal influence on the embryogenic capability of megagametophytes from controlled crosses, as well as the effect of the temperature and water availability during SE process on the production of plants. A strong maternal effect on the embryogenic potential of maritime pine megagametophytes was observed in our experiments using half-sib and full-sib progenies, while paternal effect was almost undetectable. Besides, it seems possible to improve somatic embryo production of maritime pine megagametophytes by adjusting optimal temperature throughout the process: 28°C during induction and proliferation, and 23°C during the maturation phase. Using induction and proliferation media with reduced water availability (6 g/L Gelrite) can also increase embryo production. Since other limitation of maritime pine SE is culture decline of embryogenic masses (EMs), that reduces embryo yield and germination, we assessed the profile of ABA and IAA and the expression of two embryogenesis-related genes ( LEC1 and WOX2 ) during maturation of EMs of two morphotypes that differed in their maturation capability. Spiky morphotype (SK), with high maturation capability, had a steady increase in both hormones along the 12 weeks of the maturation, whereas ABA content in smooth morphotype picked at the 4th week and dropped. EMs with this morphotype also had a higher IAA content at the beginning of the maturation. A decrease of LEC1 and WOX2 gene expression over the course of embryo development was found to be characteristic of the SK with high maturation capability.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

et al. 2019

View full text Add to dashboard Cite

show abstract

“…DNA methylation is important for somatic embryo development (Nic-Can et al, 2013; Yakovlev et al, 2016). In general, higher global DNA methylation has been found in non-embryogenic cultures of Pinus radiata (Bravo et al, 2017), P. nigra (Noceda et al, 2009), Rosa x hybrid (Xu et al, 2004), and Eleutherococcus senticosus (Chakrabarty et al, 2003), while low global DNA methylation has been found in embryogenic cultures of several plants. In Quercus alba DNA is demethylation during the induction of SE (Corredoira et al, 2017), as well as during the generation of pro-embryogenic mass, but it gradually increases as the embryo is developing (LoSchiavo et al, 1989).…”

Section: Epigeneticsmentioning

confidence: 99%

Signaling Overview of Plant Somatic Embryogenesis

et al. 2019

View full text Add to dashboard Cite

Somatic embryogenesis (SE) is a means by which plants can regenerate bipolar structures from a somatic cell. During the process of cell differentiation, the explant responds to endogenous stimuli, which trigger the induction of a signaling response and, consequently, modify the gene program of the cell. SE is probably the most studied plant regeneration model, but to date it is the least understood due to the unclear mechanisms that occur at a cellular level. In this review, the authors seek to emphasize the importance of signaling on plant SE, highlighting the interactions between the different plant growth regulators (PGR), mainly auxins, cytokinins (CKs), ethylene and abscisic acid (ABA), during the induction of SE. The role of signaling is examined from the start of cell differentiation through the early steps on the embryogenic pathway, as well as its relation to a plant’s tolerance of different types of stress. Furthermore, the role of genes encoded to transcription factors (TFs) during the embryogenic process such as the LEAFY COTYLEDON (LEC), WUSCHEL (WUS), BABY BOOM (BBM) and CLAVATA (CLV) genes, Arabinogalactan-proteins (AGPs), APETALA 2 (AP2) and epigenetic factors is discussed.

show abstract

“…A number of studies have already compared embryogenic and non-embryogenic tissues of several coniferous genera ( Araucaria, Larix, Picea , and Pinus spp.) in terms of targeted gene expression (including miRNA), DNA methylation, and transcriptomic or proteomic profiling (reviewed in Miguel et al, 2016; Bravo et al, 2017; Klubicová et al, 2017). Putative marker genes of embryogenic state have been highlighted, including some that are reportedly involved in embryogenic induction in higher plants (Elhiti et al, 2013; Mahdavi-Darvari et al, 2015), particularly totipotency and commitment to somatic embryogenesis, e.g., WUS / WOX2, LEC1 / CHAP3A and BBM / SAP2C (Miguel et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Pseudotsuga menziesii [Mirb.])

Gautier

Label²,

Eliášová

et al. 2019

Front. Plant Sci.

View full text Add to dashboard Cite

Somatic embryogenesis techniques have been developed for most coniferous species, but only using very juvenile material. To extend the techniques’ scope, better integrated understanding of the key biological, physiological and molecular characteristics of embryogenic state is required. Therefore, embryonal masses (EMs) and non-embryogenic calli (NECs) have been compared during proliferation at multiple levels. EMs and NECs originating from a single somatic embryo (isogenic lines) of each of three unrelated genotypes were used in the analyses, which included comparison of the lines’ anatomy by transmission light microscopy, transcriptomes by RNAseq Illumina sequencing, proteomes by free-gel analysis, contents of endogenous phytohormones (indole-3-acetic acid, cytokinins and ABA) by LC-MS analysis, and soluble sugar contents by HPLC. EMs were characterized by upregulation (relative to levels in NECs) of transcripts, proteins, transcription factors and active cytokinins associated with cell differentiation accompanied by histological, carbohydrate content and genetic markers of cell division. In contrast, NECs were characterized by upregulation (relative to levels in EMs) of transcripts, proteins and products associated with responses to stimuli (ABA, degradation forms of cytokinins, phenols), oxidative stress (reactive oxygen species) and carbohydrate storage (starch). Sub-Network Enrichment Analyses that highlighted functions and interactions of transcripts and proteins that significantly differed between EMs and NECs corroborated these findings. The study shows the utility of a novel approach involving integrated multi-scale transcriptomic, proteomic, biochemical, histological and anatomical analyses to obtain insights into molecular events associated with embryogenesis and more specifically to the embryogenic state of cell in Douglas-fir.

show abstract

Differences in DNA methylation, DNA structure and embryogenesis-related gene expression between embryogenic and non embryogenic lines of Pinus radiata D. don

Cited by 24 publications

References 53 publications

New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

New Approaches to Optimize Somatic Embryogenesis in Maritime Pine

Signaling Overview of Plant Somatic Embryogenesis

Cytological, Biochemical and Molecular Events of the Embryogenic State in Douglas-fir (Pseudotsuga menziesii [Mirb.])

Contact Info

Product

Resources

About