Differences in Estimates of Cisplatin-Induced Cell Kill in Vitro Between Colorimetric and Cell Count/Colony-Assays

Henriksson, Eva; Kjellén, Elisabeth; Wahlberg, Peter; Wennerberg, Johan; Kjellström, Johan

doi:10.1290/0604022

Cited by 20 publications

(21 citation statements)

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“…Apoptosis is a process that constitutes a natural barrier for tumor development, and its induction in neoplastic cells is one of cancer chemopreventive and chemotherapeutic strategies [17]. We investigated the proapoptotic activity of EPFP towards MCF-7 cells by means of flow cytometry.…”

Section: Resultsmentioning

confidence: 99%

Differentiated impact of procyanidins from evening primrose on human breast cancer cells

et al. 2014

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This study examines some of the biological activities of an evening primrose flavanol preparation (EPFP) against non-invasive human breast cancer cells (MCF-7). The results are compared with those obtained for highly invasive human breast cancer cells (MDAMB-231). The results show, for the first time, that EPFP reduces MCF-7 cell number, IC50 = 75 µM gallic acid equivalents/GAE for 72 h incubation, and reduces migration to 52% of the control value at 100 µM L−1 GAE. EPFP caused favorable changes in Bcl-2/Bax mRNA ratio, which rendered MCF-7 cells more sensitive to apoptosis: the number of apoptotic cells increased 2.2-fold vs. control at 100 µM GAE. Furthermore, 100 µ M L−1 GAE EPFP caused a 1.8-fold reduction in the activity of metalloproteinase-9 (MMP-9) secreted to the culture medium by MCF-7 cells. Moreover, EPFP suppressed the expression of selected genes of matrix metalloproteinases (MMPs), a proliferation marker (Ki67), and vascular endothelial growth factor (VEGF). In conclusion, the results of this study suggest that EPFP may exhibit proapoptotic, antiproliferative, antimigratory, and antimetastatic potential towards both selected human breast cancer cell lines, which is more pronounced in the case of the highly invasive MDA-MB-231 cells.

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Section: Resultsmentioning

confidence: 99%

Differentiated impact of procyanidins from evening primrose on human breast cancer cells

et al. 2014

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“…Furthermore, some drug treatments influence mitochondrial oxidative potential. The colony-forming assay, on the other hand, measures a drug's ability to induce clonogenic cell death [16,17]. With the use of this assay, continuous exposure to trastuzumab for 7 and 10 days was found to have produced only a marginal decrease in colony formation after treatment of MDA-MB-468 cells with the apparent IC 20 determined using the MTT assay, suggesting that treatment with trastuzumab would not be therapeutically effective as expected for HER-2-negative cells.…”

Section: Discussionmentioning

confidence: 99%

Changes in 2-fluoro-2-deoxy-d-glucose incorporation, hexokinase activity and lactate production by breast cancer cells responding to treatment with the anti-HER-2 antibody trastuzumab

Cheyne

Trembleau

Mclaughlin

et al. 2011

Nuclear Medicine and Biology

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“…Crystal violet staining (CVS) was performed according to Henriksson et al [2006] with slight modifi cations. Briefl y, the cells were harvested, suspended in the growth medium mentioned above and seeded on 96-well plates.…”

Section: Crystal Violet Stainingmentioning

confidence: 99%

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

Szewczyk¹,

Lewandowska²,

Owczarek³

et al. 2014

Pol. J. Food Nutr. Sci.

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Key words: evening primrose, matrix metalloproteinases, cancer Evening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid --linolenic acid (GLA) -but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the infl uence of a polyphenol extract from defatted seeds of evening primrose on human colorectal adenocarcinoma Caco-2 cell proliferation and matrix metalloproteinases (MMPs) synthesis and activity. To assess the effect of evening primrose extract on Caco-2 cell proliferation, crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluated by RT-PCR and gelatin zymography.The results revealed that the examined polyphenol extract had little infl uence on Caco-2 proliferation, but effectively in a time-and dose-dependent manner inhibited MMP-1, MMP-7, MMP-9 and MMP-14 mRNA synthesis induced by TNF- and TPA. Additionally, zymographic analysis revealed that after 24 h, the polyphenol extract at a concentration of 50 mol/L GAE caused a 10-fold reduction in MMP-9 synthesis. Moreover, this extract might be a potent inhibitor of MMP activity. The results showed that polyphenol extract from evening primrose inhibited PBMC-derived MMP-2 and MMP-9 enzymatic activity in dose-dependent manner. The obtained results indicate that the polyphenol extract from evening primrose seeds could be an inhibitor of proteases involved in tumor progression and metastasis.

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Differences in Estimates of Cisplatin-Induced Cell Kill in Vitro Between Colorimetric and Cell Count/Colony-Assays

Cited by 20 publications

References 0 publications

Differentiated impact of procyanidins from evening primrose on human breast cancer cells

Differentiated impact of procyanidins from evening primrose on human breast cancer cells

Changes in 2-fluoro-2-deoxy-d-glucose incorporation, hexokinase activity and lactate production by breast cancer cells responding to treatment with the anti-HER-2 antibody trastuzumab

Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors

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