Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition in<i>Plantago</i>spp.

Phan, Jana L.; Tucker, Matthew R.; Khor, Shi F.; Shirley, Neil J.; Lahnstein, Jelle; Beahan, Cherie T.; Bacic, Antony; Burton, Rachel A.

doi:10.1093/jxb/erw424

Cited by 43 publications

(101 citation statements)

References 62 publications

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“…Flax (Linum usitatissimum) and chia (Salvia hispanica) seeds were purchased from Woolworths (Frewville, South Australia). Plantago ovata and Plantago cunninghamii seeds were obtained and bulked from sources listed in Phan et al [30]. P. ovata varieties were grown in field trials conducted in 2017 and 2018 in Kununurra, Western Australia.…”

Section: Methodsmentioning

confidence: 99%

A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

et al. 2020

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Background: Myxospermy is a process by which the external surfaces of seeds of many plant species produce mucilage-a polysaccharide-rich gel with numerous fundamental research and industrial applications. Due to its functional properties the mucilage can be difficult to remove from the seed and established methods for mucilage extraction are often incomplete, time-consuming and unnecessarily wasteful of precious seed stocks. Results: Here we tested the efficacy of several established protocols for seed mucilage extraction and then downsized and adapted the most effective elements into a rapid, small-scale extraction and analysis pipeline. Within 4 h, three chemically-and functionally-distinct mucilage fractions were obtained from myxospermous seeds. These fractions were used to study natural variation and demonstrate structure-function links, to screen for known mucilage quality markers in a field trial, and to identify research and industry-relevant lines from a large mutant population. Conclusion: The use of this pipeline allows rapid analysis of mucilage characteristics from diverse myxospermous germplasm which can contribute to fundamental research into mucilage production and properties, quality testing for industrial manufacturing, and progressing breeding efforts in myxospermous crops.

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Section: Methodsmentioning

confidence: 99%

A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

et al. 2020

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“…2.1 Materials P. ovata seeds were provided by Dr Matthew Tucker (University of Adelaide, Australia). The plant growth condition was based on a report of Phan et al (2016). Briefly, the seeds are sterilized with 50% ethanol and then washed with water before they are germinated on agar plates at 22 °C/18 °C.…”

Section: Methodsmentioning

confidence: 99%

Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology

Yakubov

Gilbert

et al. 2019

Carbohydrate Polymers

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“…During seed germination, many catabolic reactions occur in endosperm cells to provide energy and substrates for seed germination, and a large number of enzymes involved in metabolism are synthesized or degraded, and PCD occurs in aleurone layer cells [12]. The glycosyltransferase family 61 protein (GT61) has been confirmed to be involved in the synthesis of xylan, one of the main components of the cell wall [37]. In the putative model (Figure 7), GT61 was down-regulated by salt stress, which could result in decreasing the cell wall formation along with the PCD pathway in aleurone layer cells.…”

Section: Energy and Substrates Supplied By Endosperm Under Salt Stressmentioning

confidence: 99%

Integrative Transcriptomic and Proteomic Analyses of Molecular Mechanism Responding to Salt Stress during Seed Germination in Hulless Barley

Lai

Zhang

Wang

et al. 2020

IJMS

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Hulless barley (Hordeum vulgare L. var. nudum) is one of the most important crops in the Qinghai-Tibet Plateau. Soil salinity seriously affects its cultivation. To investigate the mechanism of salt stress response during seed germination, two contrasting hulless barley genotypes were selected to first investigate the molecular mechanism of seed salinity response during the germination stage using RNA-sequencing and isobaric tags for relative and absolute quantitation technologies. Compared to the salt-sensitive landrace lk621, the salt-tolerant one lk573 germinated normally under salt stress. The changes in hormone contents also differed between lk621 and lk573. In lk573, 1597 differentially expressed genes (DEGs) and 171 differentially expressed proteins (DEPs) were specifically detected at 4 h after salt stress, and correspondingly, 2748 and 328 specifically detected at 16 h. Most specific DEGs in lk573 were involved in response to oxidative stress, biosynthetic process, protein localization, and vesicle-mediated transport, and most specific DEPs were assigned to an oxidation-reduction process, carbohydrate metabolic process, and protein phosphorylation. There were 96 genes specifically differentially expressed at both transcriptomic and proteomic levels in lk573. These results revealed the molecular mechanism of salt tolerance and provided candidate genes for further study and salt-tolerant improvement in hulless barley.

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Differences in glycosyltransferase family 61 accompany variation in seed coat mucilage composition inPlantagospp.

Abstract: HighlightSeed coat mucilage composition and heteroxylan structure varies between different Plantago species and is accompanied by differences in glycosyltransferase family 61 (GT61) copy number and transcript abundance.

Cited by 43 publications

References 62 publications

A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

A small-scale fractionation pipeline for rapid analysis of seed mucilage characteristics

Multi-scale assembly of hydrogels formed by highly branched arabinoxylans from Plantago ovata seed mucilage studied by USANS/SANS and rheology

Integrative Transcriptomic and Proteomic Analyses of Molecular Mechanism Responding to Salt Stress during Seed Germination in Hulless Barley

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