Differences in Grain Ultrastructure, Phytochemical and Proteomic Profiles between the Two Contrasting Grain Cd-Accumulation Barley Genotypes

Sun, Hongyan; Cao, Fengming; Wang, Nanbo; Zhang, Mian; Ahmed, Imrul Mosaddek; Zhang, Guoping; Wu, Feibo

doi:10.1371/journal.pone.0079158

Cited by 19 publications

(9 citation statements)

References 42 publications

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“…The total nitrogen content in grain was quantified according to the Kjeldahl method by using FOSS Kjeltec TM 2300 (Foss Analytical AB, Sweden; Kjeldahl, 1883 ). GPC was calculated by using the following formula: GPC = Nitrogen content × 5.83 × 100% ( Sun et al, 2013 ). Statistical analysis of the differences in aerial part traits between cultivars was performed by using Student’s t -test.…”

Section: Methodsmentioning

confidence: 99%

Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content

et al. 2016

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Grain protein contents (GPCs) of barley seeds are significantly different between feed and malting barley cultivars. However, there is still no insight into the proteomic analysis of seed proteins between feed and malting barley cultivars. Also, the genetic control of barley GPC is still unclear. GPCs were measured between mature grains of Yangsimai 3 and Naso Nijo. A proteome profiling of differentially expressed protein was established by using a combination of 2-DE and tandem mass spectrometry. In total, 502 reproducible protein spots in barley seed proteome were detected with a pH range of 4–7 and 6–11, among these 41 protein spots (8.17%) were detected differentially expressed between Yangsimai 3 and Naso Nijo. Thirty-four protein spots corresponding to 23 different proteins were identified, which were grouped into eight categories, including stress, protein degradation and post-translational modification, development, cell, signaling, glycolysis, starch metabolism, and other functions. Among the identified proteins, enolase (spot 274) and small subunit of ADP-glucose pyrophosphorylase (spot 271) are exclusively expressed in barley Yangsimai 3, which may be involved in regulating seed protein expression. In addition, malting quality is characterized by an accumulation of serpin protein, Alpha-amylase/trypsin inhibitor CMb and Alpha-amylase inhibitor BDAI-1. Most noticeably, globulin, an important storage protein in barley seed, undergoes post-translational processing in both cultivars, and also displays different expression patterns.

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Section: Methodsmentioning

confidence: 99%

Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content

et al. 2016

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“…The total nitrogen (N) content was then quantified by using FOSS KjeltecTM 2300 (Foss Analytical AB). Grain protein content was calculated with the following formula: GPC = N content × 5.83 × 100% (Sun et al., 2013). The content of β‐glucan was measured using Megazyme assay kits (Megazyme International), according to the manufacturer's instruction.…”

Section: Methodsmentioning

confidence: 99%

Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

et al. 2021

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Hullessbarley (Hordeum vulgare L.) is a barley cultivar, serving as one of the major food crops worldwide. The main nutrients in hulless barley are starch, protein, and βglucan, the contents of which vary considerably among different cultivars. However, the potential molecular mechanisms that underlie these variations in nutrient are still insufficiently clear. In this study, the nutrient content of two hulless barley cultivars ('Zangqing 2000' and 'C2') was analyzed, and quantitative tandem mass tag-LC-MS/MS proteomics and bioinformatics analysis were used to verify the differentially expressed proteins (DEPs) between the two cultivars. Parallel reaction monitoring (PRM) analysis was used to verify the expression change of candidate DEPs. The content of starch and amylose in C2 was lower than that in Zangqing 2000, and βglucan, protein content, and amylopectin showed a higher concentration in C2 hulless barley cultivar. A total of 326 DEPs were identified, with 108 induced proteins and 218 reduced proteins in C2 compared with Zangqing 2000. Further functional and pathways analysis of DEPs revealed that C2 hulless barley cultivar has relatively more biological activities, and DEPs in C2 hulless barley cultivar were mainly associated with the biological processes of multiple important metabolic pathways. Moreover, PRM analysis showed the accuracy level of quantitative proteomic data. Overall, this was the first study that undertook the proteomic analysis in Zangqing 2000 and C2 and the results revealed the molecular mechanisms of seed nutrient biosynthesis and accumulation, which provide key insights about the development of hulless barley quality.

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“…The nitrogen content and protein composition of whole grain samples were quantified according to the Kjeldahl method using a FOSS Kjeltec TM 2300 analyzer unit (Foss Analytical AB, Sweden) [19]. Protein content (grain protein content, albumin content, globulin content, hordein content and gluten content) was calculated using the following formula: Protein content = Nitrogen content×5.83×100% [20]. Statistical analysis of the differences in aerial part traits between cultivars was performed using Student’s t -tests.…”

Section: Methodsmentioning

confidence: 99%

Regulation of nitrogen availability results in changes in grain protein content and grain storage subproteomes in barley (Hordeum vulgare L.)

Guo

Lin

et al. 2019

PLoS ONE

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Barley grain protein content (GPC) is an important quality factor that determines grain end-use value. The synthesis and accumulation of grain protein is highly dependent on the availability of nitrogen fertilizer, and it is important to understand the underlying control mechanisms of this. In the current study, the GPC and protein composition of mature grain seeds from Yangsimai 3 and Naso Nijo barley cultivars were analyzed. Grain storage subproteomes (albumin, glubulin, hordein and glutelin) were compared in the cultivars grown in both low and high nitrogen level conditions. The GPC of mature grain was significantly higher in Yangsimai 3 than Naso Nijo following nitrogen treatment. Albumin, hordein and glutelin content were increased in Yangsimai, while only hordein content was increased in Naso Nijo. Large-scale analysis of the grain storage subproteome revealed 152 differentially expressed protein spots on 2-DE gels with a pH range of 3–10. Among these, 42 and 66 protein spots were successfully identified by tandem mass spectrometry in Yangsimai 3 and Naso Nijo grown in low and high nitrogen conditions. The identified proteins were further grouped into thirteen categories according to their biological functions. This detailed analysis of grain subproteomes provides information on how barley GPC may be controlled by nitrogen supply.

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Differences in Grain Ultrastructure, Phytochemical and Proteomic Profiles between the Two Contrasting Grain Cd-Accumulation Barley Genotypes

Cited by 19 publications

References 42 publications

Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content

Comparative Proteomic Analysis of Two Barley Cultivars (Hordeum vulgare L.) with Contrasting Grain Protein Content

Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

Regulation of nitrogen availability results in changes in grain protein content and grain storage subproteomes in barley (Hordeum vulgare L.)

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